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Preparation of high density lipoprotein cholesterol detection kit with strong antijamming capability

A high-density lipoprotein and detection kit technology, applied in biological testing, measuring devices, material testing products, etc., can solve the problems of poor stability and weak anti-interference ability of the kit, and achieve enhanced anti-interference ability and increased response. The effect of improving speed and stability

Active Publication Date: 2018-07-06
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Similar products currently on the market are generally enzymatic detection kits, but such kits generally have problems such as poor stability and weak anti-interference ability

Method used

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  • Preparation of high density lipoprotein cholesterol detection kit with strong antijamming capability
  • Preparation of high density lipoprotein cholesterol detection kit with strong antijamming capability
  • Preparation of high density lipoprotein cholesterol detection kit with strong antijamming capability

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] The reagent R1 consists of:

[0014] Polyanionic cellulose 0.5mmol / L

[0015] Cholesterol oxidase 0.8KU / L

[0016] Peroxidase 1.5KU / L

[0017] TTAB 0.25mmol / L

[0018] PIPES buffer 50mmol / L

[0019] Tetradecyl Betaine 0.5g / L

[0020] Chloroform 5ml / L

[0021] Protamine Sulfate 5g / L

[0022] Reagent R2 consists of:

[0023] 4-antipyrine 25mmol / L

[0024] Cholesterol lipase 3KU / L

[0025] Tween-20 2ml / L

[0026] Mannitol 10g / L

[0027] Glycerol 5ml / L

[0028] GOODS buffer 75mmol / L.

example 2

[0030] Reagent R1 consists of:

[0031] Polyanionic cellulose 0.5mmol / L

[0032] Cholesterol oxidase 0.8KU / L

[0033] Peroxidase 1.5KU / L

[0034] TTAB 0.25mmol / L

[0035] PIPES buffer 50mmol / L

[0036] Tetradecyl Betaine 1.5g / L

[0037] Chloroform 2.5ml / L

[0038] Protamine Sulfate 5g / L

[0039] Reagent R2 consists of:

[0040] 4-antipyrine 25mmol / L

[0041] Cholesterol lipase 3KU / L

[0042] Tween-20 5ml / L

[0043] Mannitol 10g / L

[0044] Glycerol 2ml / L

[0045] GOODS buffer 75mmol / L.

example 3

[0047] Reagent R1 consists of:

[0048] Polyanionic cellulose 0.5mmol / L

[0049] Cholesterol oxidase 0.8KU / L

[0050] Peroxidase 1.5KU / L

[0051] TTAB 0.25mmol / L

[0052] PIPES buffer 50mmol / L

[0053] Tetradecyl Betaine 3.5g / L

[0054] Chloroform 3.5ml / L

[0055] Protamine Sulfate 7.5g / L

[0056] Reagent R2 consists of:

[0057] 4-antipyrine 25mmol / L

[0058] Cholesterol lipase 3KU / L

[0059] Tween-20 2-5ml / L

[0060] Mannitol 10g / L

[0061] Glycerol 5ml / L

[0062] GOODS buffer 75mmol / L.

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Abstract

The invention discloses preparation method of a high density lipoprotein cholesterol detection kit with strong antijamming capability and aims at providing the high density lipoprotein cholesterol detection kit which has strong stability and antijamming capability, convenience in storage and transportation and high accuracy and precision. The kit disclosed by the invention is a liquid type dual-reagent in-vitro detection kit, and the reagents mainly include a reagent R1 and a reagent R2. The high density lipoprotein cholesterol detection kit disclosed by the invention is based on a traditionalformula; matters of chloroform, protamine sulfate, tetradecyl betane, mannitol, glycerol and the like are guided into the reagents, so that the reagents are qualitatively changed and have stronger stability and antijamming capability.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro diagnostic reagents, in particular to a high-density lipoprotein cholesterol detection kit with strong anti-interference ability. Background technique [0002] Plasma lipoprotein is the existence and transport form of cholesterol in plasma, that is, non-polar lipids in plasma, including cholesterol, are combined with hydrophilic apolipoprotein, so that lipoprotein has strong water solubility and can be transported in blood. High-density lipoprotein, low-density lipoprotein, very low-density lipoprotein and chylogranules together constitute plasma lipoproteins, among which low-density lipoprotein is mainly responsible for transporting cholesterol to peripheral tissues, while high-density lipoprotein is responsible for clearing the arterial wall Cholesterol is transported to the liver. Frammingham's large-scale epidemiological survey in 1977 showed that the reduction of plasma high-densit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92G01N33/53
CPCG01N33/5306G01N33/92G01N2800/323G01N2800/324
Inventor 甘宜梧李建营单泉
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD