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Tissue culture seedling growing method for Centranthera grandiflora Benth

A tissue culture, wild bean technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of scarcity of wild resources of red-rooted wild bean, low natural reproduction ability of seeds, and inability to meet the needs of drug use, etc. Availability, faster germination, and shorter growth cycle

Inactive Publication Date: 2018-07-10
康美药业(昆明)种质资源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Red-rooted wild broad bean is a precious local traditional Chinese medicine. It is mainly distributed in Vietnam, Honghe Prefecture and Wenshan Prefecture in Yunnan near the Vietnamese border. At present, there are few wild resources of red-rooted wild broad bean. People excavate it too much, and there are seeds that reproduce naturally. Low capacity, difficult to survive after transplanting, etc., lead to scarcity of wild resources of red root wild broad bean
[0003] The whole plant of red root wild broad bean has medicinal value, which is recorded in "Yunnan Simao Chinese Herbal Medicine Selection" and "Xinhua Materia Medica, Yunnan Honghe Chinese Herbal Medicine Handbook"; due to the small size of the seeds, the seeds have low reproductive ability and slow reproduction speed through traditional methods , under natural conditions, the emergence rate of direct seeding is about 20%-30%, and it can only grow to a height of 2-3 cm in the first year. The growth cycle is 3-5 years, and the growth cycle is long, which cannot meet the needs of medication.

Method used

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  • Tissue culture seedling growing method for Centranthera grandiflora Benth
  • Tissue culture seedling growing method for Centranthera grandiflora Benth
  • Tissue culture seedling growing method for Centranthera grandiflora Benth

Examples

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Effect test

Embodiment 1

[0076] A method for raising seedlings by tissue culture of red-rooted broad bean, comprising:

[0077] Explant selection step: select a healthy wild red-rooted broad bean plant, let it bloom and bear fruit, after the fruit pods mature, pick and preserve the fruit pods to obtain spare fruit pods; the plant age of the red-rooted wild broad bean plant is 3 years; Select mature and intact fruit pods from mid-to-late September to early October. Since the seed shells of the fruit pods are thin and easy to be cracked or crushed, they should be carefully collected and kept.

[0078] Fruit pod disinfection steps: Take out the spare fruit pods, peel off the outermost shell of the spare fruit pods, soak the spare fruit pods with the outermost shell peeled off in detergent water for 5 minutes, and then rinse them with tap water for 3 minutes. Obtain the first clean fruit pod; place the first clean fruit pod in a beaker, soak it with 75% alcohol for 1 min, rinse it twice with sterile water...

Embodiment 2

[0087] A method for raising seedlings by tissue culture of red-rooted broad bean, comprising:

[0088] Explant selection steps: select a healthy wild red-rooted broad bean plant, let it bloom and bear fruit, after the fruit pods mature, pick and store the fruit pods to obtain spare fruit pods; the plant age of the red-rooted wild broad bean plant is 3-4 Years; select mature and intact fruit pods from mid-to-late September to early October. Since the seed shells of the pods are thin and easy to be cracked or crushed, they should be carefully collected and kept.

[0089] Fruit pod disinfection steps: Take out the spare fruit pods, peel off the outermost shell of the spare fruit pods, soak the spare fruit pods with the outermost shell peeled off in detergent water for 10 minutes, and then rinse them with tap water for 5 minutes. Obtain the first clean fruit pod; place the first clean fruit pod in a beaker, soak it with 75% alcohol for 1 min, rinse it with sterile water for the fi...

Embodiment 3

[0098] A method for raising seedlings by tissue culture of red-rooted broad bean, comprising:

[0099] Explant selection steps: select a healthy wild red-rooted broad bean plant, let it bloom and bear fruit, after the fruit pods mature, pick and store the fruit pods to obtain spare fruit pods; the plant age of the red-rooted wild broad bean plant is 3-4 Years; select mature and intact fruit pods from mid-to-late September to early October. Since the seed shells of the pods are thin and easy to be cracked or crushed, they should be carefully collected and kept.

[0100] Fruit pod disinfection steps: Take out the spare fruit pods, peel off the outermost shell of the spare fruit pods, soak the spare fruit pods with the outermost shell peeled off in detergent water for 10 minutes, and then rinse them with tap water for 5 minutes. Obtain the first clean fruit pod; place the first clean fruit pod in a beaker, soak it with 75% alcohol for 1 min, rinse it with sterile water for the fi...

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Abstract

The invention discloses a tissue culture seedling growing method for Centranthera grandiflora Benth. The method comprises the following steps: explant selection; fruit pod disinfection; seed disinfection; seed sowing; subculture; rooting culturing; seedling hardening and domestication; and transplanting. The tissue culture seedling growing method for Centranthera grandiflora Benth allows high-quality seedlings to be rapidly produced by using seeds of the Centranthera grandiflora Benth, and has the characteristics of high germination rate, short growth period, realization of batch production ofthe Centranthera grandiflora Benth, satisfying of production demands, and alleviation of the current situation of scarcity of resources.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for raising seedlings of red-rooted wild faba bean through tissue culture. Background technique [0002] Red-rooted broad bean, in the plant kingdom, angiosperms, dicotyledons, scrophulariaceae, and flax, is the whole plant of red-rooted wild broad bean centranthera cochinchinensis(lour.)merr.[digitalis cochinchinensislour.], also known as large Flower flax grass, Huaxue Dan, Xiaohongyao, wild broad beans, etc.; warm in nature, sour in taste, slightly pungent, used for dispelling blood stasis and hemostasis, reducing swelling and relieving pain; mainly treating hemoptysis, hemoptysis, hematemesis, women's amenorrhea, internal injury bleeding, Rheumatic arthralgia in middle-aged and elderly people, muscle and bone pain, cerebral congestion, cerebral thrombosis, alcoholic liver disease, etc. Red-rooted wild broad bean is a precious local traditional Chinese medicin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许冬瑾严新顾晓波杜永魏
Owner 康美药业(昆明)种质资源有限公司
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