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Application of naringin to preparation of medicine for treating and delaying intervertebral disc degeneration

A technology of naringin and intervertebral disc, which is applied in the application field of naringin in the preparation of drugs for treating and delaying intervertebral disc degeneration, can solve the problems of unclear treatment effect of intervertebral disc degeneration diseases, etc., and achieves obvious antioxidant effect and dosage of medication. Low, anti-inflammatory mitigating effect

Inactive Publication Date: 2018-07-13
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But whether it has a therapeutic effect on degenerative disc disease is unclear

Method used

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  • Application of naringin to preparation of medicine for treating and delaying intervertebral disc degeneration
  • Application of naringin to preparation of medicine for treating and delaying intervertebral disc degeneration
  • Application of naringin to preparation of medicine for treating and delaying intervertebral disc degeneration

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Experimental program
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Effect test

Embodiment Construction

[0019] 1. Experimental steps:

[0020] (1) Modeling method: Take rats for anesthesia with 2% pentobarbital sodium (40mg / kg), disinfect with iodine, take the tail vertebrae of rats and puncture them with a 27g puncture needle, puncture the full-thickness annulus fibrosus, and pull out after indwelling for 60s. Then, the rat tail intervertebral disc degeneration model was obtained. After the operation, 20mg / ml naringin (diluted in normal saline) was injected intraperitoneally at 80mg / kg / d.

[0021] (2) Primary culture: Ten rats (5 males and 5 males, 100-150 g) were sacrificed by excessive sodium pentobarbital. After the intervertebral disc and annulus fibrosus of L1-L6 were removed under sterile conditions, the nucleus pulposus was collected using a dissecting microscope. Treat with 0.1% collagenase 2mg / ml and hyaluronidase 2U / ml for 4 hours at 37°C. The digested nucleus pulposus tissue was incubated in DMEM / F12 medium containing 15% FBS and 1% double antibody at 37°C and 5% ...

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Abstract

The invention relates to application of naringin to reduction of nucleus pulposus cell apoptosis and delay of intervertebral disc degeneration and particularly relates to application of the naringin to preparation of a medicine for treating and delaying the intervertebral disc degeneration. Compared with western medicines with larger side effect, the naringin as a fruit extract is a traditional-Chinese-medicine component and has the effects that the intervertebral disc degeneration can be delayed in a milder manner, and the toxicity of medicines can be reduced.

Description

technical field [0001] The invention relates to the application of naringin, in particular to the application of naringin in the preparation of medicines for treating and delaying intervertebral disc degeneration. Background technique [0002] Low back pain is a common disease in orthopedics, and it is more likely to occur in people aged 40 to 80. Severe low back pain can make patients incapable of work and impose a heavy burden on society and families. More than 40% of low back pain is caused by disc degeneration. But so far, there is no clinically available drug for the treatment of intervertebral disc degeneration. [0003] Intervertebral disc degeneration can be caused by a variety of factors, such as abnormal stress, local nutritional deficiency, and congenital genetic abnormalities, among which oxidative stress is one of the main pathogenic mechanisms of intervertebral disc degeneration, and excessive oxidative stress can cause nucleus pulposus cell apoptosis. Death...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P19/00A61P19/08
CPCA61K31/7048
Inventor 张小磊章增杰林甲亮王晓斌
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE