Centrifugal micro-droplet particle chip

Abstract

The invention discloses a centrifugal micro-droplet particle chip. The chip comprises an upper piece and a lower piece bonded with the upper piece, a sample storage ring arranged in the middle of theouter surface of the upper piece or in the middle of the outer surface of the lower piece, M closed sample flow channels which are formed in a position between the upper piece and the lower piece andare outward distributed in a radial manner from the middle of the chip, and closed arc micro-droplet particle storage slot groups (5) which are arranged between the upper piece and the lower piece onthe left sides of the flow channels, wherein M is greater than 2; the closed arc micro-droplet particle storage slot groups comprise a plurality of arc micro-droplet particle storage slots which sharethe same center of a circle; M oil discharge flow channels which are outward distributed in a radial manner from the middle of the chip are formed in a position between the lower surface of the upperpiece and the upper surface of the lower piece; each oil discharge flow channel extends to the edge of the chip and is communicated with an oil discharge outlet at the edge of the chip. The chip hasthe characteristics of high sensitivity and high accuracy.

Description

technical field [0001] The present invention relates to the field of in vitro molecular diagnosis technology in the medical field, the field of life science research, and the field of new drug development technology, in particular to a high-resolution gene target recognition technology with excellent stability, reproducibility, and ultra-high sensitivity , Extreme precision and industrialized centrifugal micro-droplet particles are made into chips. Background technique [0002] At present, fluorescence quantitative PCR (Fluorescence Quantitative Polymerase Chain Reaction, qPCR) has developed into a key routine technology in the field of in vitro molecular diagnosis, which has greatly promoted the development of life science in the field of health care. However, fluorescence quantitative PCR quantification is only relative quantification, and its accuracy and reproducibility still cannot meet the development needs of the current field of in vitro molecular diagnostic systems ...

AI Technical Summary

Problems solved by technology

However, fluorescence quantitative PCR quantification is only relative quantification, and its accuracy and reproducibility still cannot meet the development needs of the current field of in vitro molecular diagnostic systems and biological genetics research.

In addition, due to the inhibitory effect of PCR amplification products on enzyme-catalyzed reactions, the current gene variation detection method of qPCR technology is often powerless for low-abundance tumor gene variation in somatic cells

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