Method for simultaneously preparing high-purity carnosol and carnosic acid from rosemary

A technology of carnosol and carnosic acid is applied in the separation field of SephadexLH-20 gel column chromatography, which can solve the problems of waste, loss, resource waste and the like, and achieve the effect of simple and easy operation.
CN108276271AActive Publication Date: 2018-07-13BEIJING UNION UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
BEIJING UNION UNIVERSITY
Publication Date
2018-07-13

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Abstract

The invention relates to a method for simultaneously preparing high-purity carnosol and carnosic acid from rosemary. The method comprises the steps of extracting the flowers and leaves of the rosemarywith an ethanol aqueous solution to obtain a rosemary extract, and dissolving the extract in water saturated ethyl acetate; separating the obtained solution with positive phase silica gel, carrying out gradient elution according to a ratio of chloroform to methanol to acetic acid being equal to (100-95) to (0-5) to (0-0.001), merging the same components, and carrying out vacuum concentration to obtain crude products of the carnosol and the carnosic acid; then, separately carrying out Sephadex LH-20 gel column chromatography purification on the crude products, eluting a mobile phase with chloroform-methanol or petroleum ether-chloroform-methanol, merging the same components, and drying by distillation under the reduced pressure to obtain the carnosol and the carnosic acid which have the purities of 98% or above. The method can be used for simultaneously preparing the high-purity carnosol and carnosic acid from the rosemary. A gel column can be repeatedly used, and column chromatographyis short in time and high in efficiency. The method is simple, easy to operate and high in yield, thus being suitable for large-scale production.
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Description

technical field

[0001] The invention relates to a method for simultaneously preparing high-purity natural antioxidant active ingredients carnosol and carnosic acid from rosemary, in particular to a method that has a significant separation effect on the separation of a large amount of carnosol and carnosic acid Sephadex LH-20 gel column chromatography separation method. Background technique

[0002] Rosemary is a natural antioxidant, and carnosol and carnosic acid are its main antioxidant components. With the rapid development of the functional food industry, the demand for edible antioxidant products is increasing; And along with the successful introduction of rosemary to my country and its large-scale cultivation, finding a simple and cheap method for preparing high-purity antioxidant active monomers from rosemary or its extract will have good use value and Practical significance.

[0003] Many patents have reported the extraction method of antioxidant active substances in ...

Claims

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