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Indel molecular marker and application of the gene grmzm2g098557 related to the number of rows of maize ears

A GRMZM2G098557, molecular marker technology, applied in the field of molecular genetics, can solve the problems of less work and slowness in key gene mining, and achieve the effect of accelerating the breeding process and high application value

Active Publication Date: 2021-04-27
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some progress has been made in gene mining of corn ear row number, it is still relatively slow overall, and most of the genes related to corn ear row number that have been cloned so far are from mutant studies, and the map-based cloning method is used to obtain, which is of great significance to breeding practice. Mining work on key genes with high utility value is still rare

Method used

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  • Indel molecular marker and application of the gene grmzm2g098557 related to the number of rows of maize ears
  • Indel molecular marker and application of the gene grmzm2g098557 related to the number of rows of maize ears

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. The acquisition of InDel molecular markers that are significantly related to the corn gene GRMZM2G098557 and the ear row number trait.

[0023] The method of obtaining is as follows:

[0024] 1) Collect 310 temperate, tropical and subtropical maize inbred lines from China, the United States, Mexico and other places, and construct the related populations used for mapping. This population is rich in genetic diversity.

[0025] 2) Field experiment design. In 2016, they were planted in three environments in Xishuangbanna Prefecture, Yunnan (the experimental base of the Corn Institute of Sichuan Agricultural University), Ya'an, Sichuan (the experimental base of the Corn Institute of Sichuan Agricultural University), and Hongya, Sichuan. The design scheme of the field experiment adopts a randomized block experimental design, with two replicates for each design. Each family material is planted in two rows, with a row length of 4 meters and a row spacing of 0.8 met...

Embodiment 2

[0035] Example 2. The application test of the InDel marker of the present invention on the rows of corn ears.

[0036] Select 16 family materials with different phenotypes in the linkage population, use their genomic DNA as a template, and use the flanking sequence of the InDel molecular marker site to design specific primers. The primer sequences are shown in SEQ ID NO.2 and 3, using KOD FX Neo high-fidelity polymerase (Toyobo (Shanghai) Biotechnology Co., Ltd.) for PCR amplification, the program is: 94 ° C pre-denaturation 2min; 98 ° C denaturation 10s; 59 ° C annealing 30s; 68 ° C extension 6s; a total of 35 cycles; Extend at 68°C for another 10 min. The specific target band was recovered and purified with a gel recovery kit (Omega Bio-Tek), and connected to the blunt-end cloning vector Peasy-Blunt Cloning Vector (Beijing Quanshijin Biotechnology Co., Ltd.) for sequencing. Utilize SnapGene2.3.2 software to compare the sequencing results (see figure 1 ). The results showe...

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Abstract

The present invention provides the InDel molecular marker and application of the corn ear row number related gene GRMZM2G098557, which is located in the corn genome Chr4: 199959398bp, and the allele is 5bp insertion / deletion. The InDel molecular marker is significantly related to the corn ear row number, and the allele is 5bp insertion / deletion, there are two homozygous genotypes of insertion / deletion in the tested inbred line, the sequence of the 5bp is shown in SEQ ID NO.1, and its flanking sequence is shown in SEQ ID NO.2 Show. The molecular marker of the invention can be used in maize assisted breeding, and has great significance for accelerating the high-yield breeding process of maize.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to molecular markers related to corn ear row number traits, in particular to the InDel molecular marker and application of the GRMZM2G098557 gene on chromosome 4 of corn. Background technique [0002] Corn is currently the largest food crop in my country, and it is also an important industrial raw material and energy crop. Continuously increasing corn production is an important guarantee to ensure my country's food security and rapid economic development. As the area of ​​cultivated land in our country continues to decrease, it is currently the most effective way to increase corn production by increasing the yield per unit area of ​​corn. The yield per unit area of ​​corn is mainly determined by the number of ear rows, number of grains per row and 100-kernel weight, so increasing the number of corn ear rows is one of the main ways to increase corn yield per unit area. [0003] Rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 马浪浪张晓祥沈亚欧管中荣李昭玲张严玲刘鹏刘敏谭小龙张银超
Owner SICHUAN AGRI UNIV