Self circulation organ chip dynamic culture device driven by cardiac muscle cell micro pump

Abstract

The invention provides a self circulation organ chip dynamic culture device driven by a cardiac muscle cell micro pump. The device comprises a lower layer chip, a middle film and an upper layer chip in sequential laminated arrangement from bottom to top, wherein the upper layer chip comprises a cardiac muscle cell micro pump module; the lower layer chip comprises a self circulation system module and an organ chip culture module; the self circulation system module is communicated with the organ chip culture module; the cardiac muscle cell micro pump module is used for simulating the blood pumping function of the heart; the self circulation system module is used for simulating the human body blood circulation loop; the organ chip culture module is used for simulating the human body organ function; the vertical vibration of the middle film is driven through the independent beating of the cardiac muscle cells growing in the cardiac muscle cell micro pump module; the self circulation flowing of culture liquid in the self circulation system module is realized; nutrient substances of the organ chip culture module are dynamically supplied. The self circulation organ chip dynamic culture device has the advantages that the external energy supply is not needed; the environment is more similar to the human body biomimetic environment; the size is reduced.

Description

technical field [0001] The invention relates to microfluidic chip technology, in particular to a self-circulating organ chip dynamic culture device driven by a cardiomyocyte micropump and a manufacturing method thereof. Background technique [0002] Organ chip is a bionic system that can simulate the main functions of human organs by using microfluidic technology to manufacture on microfluidic chips. It not only has the characteristics of miniaturization, integration, and low consumption of microfluidic technology, but also can construct cell pattern culture, tissue-tissue interface and organ-organ interaction, etc., thereby simulating the complex structure, microenvironment and environment of human organs. Physiological function. After rapid development in recent years, researchers have realized the construction of many human organs on microfluidic chips, such as liver on a chip, lung on a chip, intestine on a chip, kidney on a chip, blood vessel on a chip, heart on a chip...

AI Technical Summary

Problems solved by technology

The disadvantage of this cardiomyocyte micropump is that it has a complex structure and is driven by a micropump with a valve. The resistance is relatively large, which is not conducive to cardiomyocytes driving liquid flow.

In 2007, Tanaka et al. used cardiomyocytes coated in a degradable spherical structure to make a spherical cardiomyocyte pump. This shape of cardiomyocyte pump can make full use of the contractile force of cardiomyocytes to generate a larger pumping force, but they did not use this myosphere pump to achieve one-way flow of liquid, only to drive the liquid to vibrate

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