Yak bone protein peptide capable of relieving fatigue and resisting oxidation and preparation method thereof

A technology for relieving fatigue and anti-oxidation, which is applied to the preparation method of peptides, chemical instruments and methods, peptide/protein components, etc., which can solve the problems of few and unseen patent applications for anti-fatigue functional protein peptides, and achieve good flavor , good antioxidant function, good functional properties

Active Publication Date: 2018-07-31
国肽生物工程(常德)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many patent applications on the anti-oxidation, ACE inhibition and hypoglycemic functions of protein peptides in domestic patents, but few patent applications on protein peptides with anti-fatigue functions
In particular, there are few reports on protein peptides that have both anti-fatigue and anti-oxidation functions
There are no patent documents and related research reports on the preparation of protein peptides with anti-fatigue and anti-oxidation functions using yak bone as raw material

Method used

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  • Yak bone protein peptide capable of relieving fatigue and resisting oxidation and preparation method thereof
  • Yak bone protein peptide capable of relieving fatigue and resisting oxidation and preparation method thereof
  • Yak bone protein peptide capable of relieving fatigue and resisting oxidation and preparation method thereof

Examples

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Effect test

Embodiment 1

[0050] This embodiment relates to a preparation method of yak bone protein peptide with anti-fatigue and anti-oxidation functions, comprising the following steps:

[0051] 1) Choose 100 grams of frozen fresh yak bones, wash them with clean water that meets the sanitary standards for drinking water, and use a beef bone crusher to beat the bones into bone particles after cleaning the bones, add water twice the total amount of yak bones at 121 After processing for 5 hours under the condition of ℃, the bovine bone cooking liquid without bone slag was obtained by centrifugal filtration, cooled to 20 ℃, and degreased through a liquid separation tank to obtain bovine bone protein liquid.

[0052] 2) Adjust the temperature of the yak bone protein solution obtained in 1) to 65°C, use an ultrasonic generator to process it with ultrasonic waves (frequency 80kH) for 15 minutes, and then pre-homogenize it once with a 20MPa homogenizer, and then heat it under a pressure of 160Mpa. Ultra-hig...

Embodiment 2

[0059] This embodiment relates to a preparation method of yak bone protein peptide with anti-fatigue and anti-oxidation functions, comprising the following steps:

[0060] 1) Choose 500 grams of frozen fresh yak bones, wash them with clean water that meets the sanitary standards for drinking water, and use a beef bone grinder to beat the bones into bone particles after cleaning the bones. After processing for 3 hours under the condition of ℃, the bovine bone cooking liquid without bone slag was obtained by centrifugal filtration, cooled to 30 ℃, and degreased through a liquid separation tank to obtain bovine bone protein liquid.

[0061] 2) Adjust the temperature of the yak bone protein liquid obtained in 1) to 70°C, use an ultrasonic generator to process it with ultrasonic waves (frequency 70kH) for 20 minutes, and then pre-homogenize it once through a 20MPa homogenizer, and then heat it under a pressure of 220Mpa Ultra-high pressure micro-jet treatment was carried out twice ...

Embodiment 3

[0067] This embodiment relates to a preparation method of yak bone protein peptide with anti-fatigue and anti-oxidation functions, comprising the following steps:

[0068] 1) Choose 1000 grams of frozen fresh yak bones, wash them with clean water that meets the drinking water hygiene standards, and use a beef bone grinder to beat the bones into bone particles after cleaning the bones, add 3.0 times the total amount of bovine bone particles in water at 125 ℃ for 3 hours, then centrifuged to obtain the bovine bone slag-removed high-temperature cooking liquid, cooled to 30 ℃, and degreased through a liquid separation tank to obtain bovine bone protein liquid;

[0069] 2) Adjust the temperature of the bovine bone protein liquid obtained in 1) to 75°C, use an ultrasonic generator to process it with ultrasonic waves (60kH frequency) for 25 minutes, and then pre-homogenize it once with a 20MPa homogenizer, and then heat it under a pressure of 160Mpa Ultra-high pressure micro-jet trea...

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Abstract

The invention relates to a preparation method of a yak bone protein peptide capable of relieving fatigue and resisting oxidation and the protein peptide prepared therefrom. The preparation method is characterized in that yak bone protein liquid is pretreated by high-frequency ultrasonic wave, ultra-high pressure micro-jet and the like, and then subjected to enzymolysis step by step using various complex proteases without adding any acid or alkali, and finally separated by membrane separation, gel separation and reverse phase HPLC separation techniques. The preparation method of the yak bone protein peptide is simple. No acid or alkali is added for pH adjustment in the whole processing process, so that a product maintains good functional characteristics, and industrial production is easilyachieved.

Description

technical field [0001] The invention relates to the field of functional protein processing, in particular to a protein peptide with functions of relieving fatigue and anti-oxidation produced from yak bone and a production method of the protein peptide. Background technique [0002] Fatigue is a normal physiological phenomenon that must occur when the body's mental or physical strength reaches a certain stage. [0003] It is a protective response to prevent life-threatening excessive failure of the body. Anti-fatigue peptides can not only provide amino acids and energy substances necessary for the human body, but also maintain the activity of body enzymes and the stability of the internal environment, enhance antioxidant capacity, prevent neurotransmitter imbalance and many other functional properties. Anti-fatigue peptides mainly through enhancing Muscle strength, improve the body's exercise endurance, so as to quickly eliminate or delay the occurrence of fatigue, and help ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K7/06C07K1/36C07K1/34C07K1/20C07K1/16A23L33/18A61K38/08A61P39/06A61P39/00
CPCA23V2002/00A61K38/00A23L33/18A61P39/00A61P39/06C07K7/06C12P21/06A23V2250/55
Inventor 张恒罗永康刘怀高
Owner 国肽生物工程(常德)有限公司
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