Method for catalytically producing bacterial cellulose by using resting cells of acetic acid bacteria

A technology of bacterial cellulose and resting cells, which is applied in the field of microorganism and fermentation engineering, can solve the problems of high price and low yield, and achieve the effects of simplified production process and equipment requirements, simple nutritional components, and simple operation

Pending Publication Date: 2018-08-03
NANJING UNIV OF TECH
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: Aiming at the problems of low yield and high price in the production of bacterial cellulose by the current fermentation method, a method for catalyzing the synthesis of bacterial cellulose by gluconacetobacter hansenii resting cells is provided to increase the production of bacterial cellulose and reduce the production cost of bacterial cellulose. method of production cost

Method used

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  • Method for catalytically producing bacterial cellulose by using resting cells of acetic acid bacteria
  • Method for catalytically producing bacterial cellulose by using resting cells of acetic acid bacteria
  • Method for catalytically producing bacterial cellulose by using resting cells of acetic acid bacteria

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: the glucose concentration in the reaction liquid is 10g / L.

[0024] (1) Strain activation: the gluconacetobacter hansenii preserved on the subculture medium was taken out, inoculated on a new subculture medium by streak transfer, and cultured at 30° C. for 5 days.

[0025] (2) Preparation of resting cells: Transplant and culture the activated strains in the same way to obtain sufficient cultures, use an inoculation loop to pick out the single colonies and lawns formed on the plate as much as possible, and place them in normal saline , use a vortex mixer to disperse as much as possible, then centrifuge at 10,000r / min and 4°C for 10min to collect the bacteria, wash, resuspend, and centrifuge twice, and then store in the refrigerator for later use.

[0026] (3) Preparation of the reaction solution: Weigh 10 g of glucose and 2.7 g of disodium hydrogen phosphate, simply dilute to 1 L with distilled water, adjust the pH to about 6.7, and sterilize at 115° C. for...

Embodiment 2

[0034] Embodiment 2: the glucose concentration in the reaction liquid is 15g / L.

[0035] (1) Strain activation: the gluconacetobacter hansenii preserved on the subculture medium was taken out, inoculated on a new subculture medium by streak transfer, and cultured at 30° C. for 5 days.

[0036] (2) Preparation of resting cells: Transplant and culture the activated strains in the same way to obtain sufficient cultures, use an inoculation loop to pick out the single colonies and lawns formed on the plate as much as possible, and place them in normal saline , use a vortex mixer to disperse as much as possible, then centrifuge at 10,000r / min and 4°C for 10min to collect the bacteria, wash, resuspend, and centrifuge twice, and then store in the refrigerator for later use.

[0037] (3) Preparation of the reaction solution: Weigh 15 g of glucose and 2.7 g of disodium hydrogen phosphate, simply dilute to 1 L with distilled water, adjust the pH to about 6.7, and sterilize at 115° C. for...

Embodiment 3

[0046] Embodiment 3: the glucose concentration in the reaction liquid is 20g / L.

[0047] (1) Strain activation: the gluconacetobacter hansenii preserved on the subculture medium was taken out, inoculated on a new subculture medium by streak transfer, and cultured at 30° C. for 5 days.

[0048] (2) Preparation of resting cells: Transplant and culture the activated strains in the same way to obtain sufficient cultures, use an inoculation loop to pick out the single colonies and lawns formed on the plate as much as possible, and place them in normal saline , use a vortex mixer to disperse as much as possible, then centrifuge at 10,000r / min and 4°C for 10min to collect the bacteria, wash, resuspend, and centrifuge twice, and then store in the refrigerator for later use.

[0049] (3) Preparation of reaction solution: Weigh 20 g of glucose and 2.7 g of disodium hydrogen phosphate, dilute to 1 L with distilled water, adjust the pH to about 6.7, and sterilize at 115° C. for 15 minutes...

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Abstract

The invention discloses a method for catalytically producing bacterial cellulose by using resting cells of acetic acid bacteria. The method comprises the following two steps: (1), preparing the resting cells: inoculating a subculture medium with the acetic acid bacteria stored in a slant form, activating, then transferring an activated culture to a flat plate to prepare a large number of single colonies or bacterial lawns, collecting the single colonies or the bacterial lawns, washing by using sterile physiological saline, resuspending and centrifuging twice to obtain the resting cells; (2), catalytically synthesizing the bacterial cellulose by using the resting cells of the acetic acid bacteria: performing static culture under a certain condition to obtain a bacterial cellulose membrane after completion of the culture. The method has the advantages of simple and controllable reaction process, high catalytic efficiency, environment friendliness, economy and the like, and by the method,an important industrial value and relatively high economic benefits are achieved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and fermentation engineering, and relates to a synthesis method of bacterial cellulose, in particular to a method for catalyzing the production of bacterial cellulose by using resting cells of acetic acid bacteria. Background technique [0002] At the end of the 19th century, British scientist Brown first discovered bacterial cellulose during experiments. Bacterial cellulose, like plant cellulose, is a macromolecule formed by connecting glucose through β-1,4-glycosidic bonds. Compared with plant cellulose, bacterial cellulose has many unique properties. First of all, bacterial cellulose has a high purity and hardly contains impurities such as hemicellulose, lignin and pectin; secondly, bacterial cellulose has a high elastic modulus, which is generally several times or even ten times that of plant cellulose , the high elastic modulus endows bacterial cellulose with high tensile strength; ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/20C12R1/02
CPCC12N1/20C12P19/04
Inventor 熊强王延斌宗丹陈海超
Owner NANJING UNIV OF TECH
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