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Phloretin preparation method

A technology of phloretin and phloretin, which is applied in the field of preparation of phloretin, can solve the problems of high cost and complicated preparation process, and achieve the effect of low cost and simple operation

Active Publication Date: 2018-08-10
CHONGQING UNIV
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  • Claims
  • Application Information

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Problems solved by technology

The above method not only has high cost, but also has a complicated preparation process. Therefore, there is an urgent need for a simple and economical method for preparing phloretin

Method used

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Embodiment 1

[0028] Phloretin

[0029] (1) Boil the leaves of Forestia wushanensis in water for 15 minutes to obtain a boiled liquid with a concentration of phloridzin of 6 mg / mL, and perform HPLC analysis after diluting the boiled liquid 30 times, the results are as follows figure 1 shown by figure 1 It can be seen that the content of phlorizin in the extract is rich and the impurities are less;

[0030] (2) Make the spore concentration 6×10 by 1% inoculation amount 7 The Trichoderma viride spore suspension of CFU / mL was inoculated in the poached liquid obtained in step (1), then fermented at 28°C, and when fermented for 5 days, an appropriate amount of fermented liquid was diluted 30 times and then analyzed by high performance liquid chromatography , the result is as figure 2 shown by figure 2 It can be seen that after 5 days of fermentation, most of the phloridzin in the extract has been converted into phloretin; then when fermented for 7 days, an appropriate amount of fermentatio...

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Abstract

The invention relates to a phloretin preparation method, and belongs to the technical field of plant extraction. The method concretely comprises the following steps of (1) poach plant materials with rich phlorizin content with water to obtain water boiling liquid; (2) inoculating green trichoderma spore suspension into the water boiling liquid obtained in the step (1); performing fermentation; after the fermentation is completed, filtering the fermentation liquid to remove thalli; so as to obtain filter liquid; firstly refrigerateing the filter liquid; then, obtaining precipitates from refrigerated filter liquid; next, purifying the precipitates to prepare phloretin solids. The method has the advantages that the operation is simple and convenient; a great number of organic solvents are notneeded; the cost is low; the green and environment-friendly effects are achieved; in addition, the finally prepared phloretin has high yield and high purity; the method is suitable for large-scale production of the phloretin.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a preparation method of phloretin. Background technique [0002] Phloretin is a dihydrochalcone compound, which has anti-oxidation, anti-inflammatory, hypoglycemic, freckle-removing and smooth skin effects. It is mainly distributed in the peel and root bark of apples, etc. It has been used for beauty and skin care product. At present, the common preparation methods of phloridin include direct extraction and separation, chemical synthesis, and chemical and biological semi-preparation using phloridin. Among them, although the direct extraction and separation method has a simple route, the content of phloretin in the extraction material itself is low, and a large amount of fillers and chemical solvents are consumed in the separation process; the yield of the chemical synthesis method is not higher than 30%, and chemical synthesis requires a large amount of raw ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/26C12R1/885
CPCC12P7/26
Inventor 夏之宁向伟郑国灿尹雄威
Owner CHONGQING UNIV
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