Industrial fermentation method of mannanase
A technology of mannanase and fermentation method, applied in the direction of microorganism-based method, glycosylase, biochemical equipment and method, etc., can solve the problems of outdated fermentation process, high cost, low benefit, etc., and achieve favorable absorption , maintain osmotic pressure, promote metabolism and produce enzymes
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Embodiment 1
[0044] Example 1. An industrial fermentation method of mannanase, characterized in that the method comprises the following steps.
[0045] Step 1: Primary fermenter fermentation
[0046] Put primary fermentation medium and polyether modified silicon defoamer in the primary fermentation tank, the volume ratio of fermentation medium and polyether modified silicon defoamer is 6000-8500:1; heat the fermentation medium , raise the temperature of the fermentation medium to 121-123°C, keep the tank pressure at 1.1-1.4MPa, and sterilize for 25-40min; after the sterilization, cool the fermentation medium in the primary fermentation tank, when the temperature drops to 29 At -32°C, transplant the fermented seed liquid into a first-stage fermenter with an inoculum volume of 5-10% by volume to expand the culture, and add trace element solution to the fermenter, the volume of the trace element solution and the fermentation medium The ratio is 1:20-25; the inoculum amount of the fermented ...
Embodiment 2
[0071] Example 2. An industrial fermentation method of mannanase, characterized in that the method comprises the following steps.
[0072] Step 1: Primary fermenter fermentation
[0073] Put primary fermentation medium and polyether modified silicon defoamer in the primary fermentation tank, the volume ratio of fermentation medium and polyether modified silicon defoamer is 6000-8500:1; heat the fermentation medium , raise the temperature of the fermentation medium to 121-123°C, keep the tank pressure at 1.1-1.4MPa, and sterilize for 25-40min; after the sterilization, cool the fermentation medium in the primary fermentation tank, when the temperature drops to 29 At -32°C, transplant the fermented seed liquid into a first-stage fermenter with an inoculum volume of 5-10% by volume to expand the culture, and add trace element solution to the fermenter, the volume of the trace element solution and the fermentation medium The ratio is 1:20-25; the inoculum amount of the fermented ...
Embodiment 3
[0100] Example 3. An industrial fermentation method of mannanase, characterized in that the method comprises the following steps.
[0101] Step 1: Primary fermenter fermentation
[0102] Put primary fermentation medium and polyether modified silicon defoamer in the primary fermentation tank, the volume ratio of fermentation medium and polyether modified silicon defoamer is 6000-8500:1; heat the fermentation medium , raise the temperature of the fermentation medium to 121-123°C, keep the tank pressure at 1.1-1.4MPa, and sterilize for 25-40min; after the sterilization, cool the fermentation medium in the primary fermentation tank, when the temperature drops to 29 At -32°C, transplant the fermented seed liquid into a first-stage fermenter with an inoculum volume of 5-10% by volume to expand the culture, and add trace element solution to the fermenter, the volume of the trace element solution and the fermentation medium The ratio is 1:20-25; the inoculum amount of the fermented ...
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