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A kind of method of grapefruit micro-bud grafting

A technology of fragrant pomelo and grafted seedlings, applied in the directions of grafting, horticultural methods, botanical equipment and methods, etc., to achieve the effects of improving the survival rate, improving the survival rate, and reducing the virus infection rate

Active Publication Date: 2022-04-19
HUNAN UNIV OF SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the application of this technology in the non-toxic seedling propagation of pomelo is still a blank

Method used

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  • A kind of method of grapefruit micro-bud grafting

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Primary medium: MS medium + 0.1 mg / L NAA and / or 0.1 mg / L BA, pH 5.6.

[0027] 2) Secondary medium: MS medium + 0.1 mg / L NAA and / or 0.1 mg / L BA + 0.6 mg / L GA, pH 5.6.

[0028] 3) Basic medium: MS medium + 6-BA 0.2mg / L + NAA 0.2mg / L + sucrose 28g / L + agar 6g / L, pH 5.6.

[0029] Taking the configuration of 1L primary medium as an example, the specific preparation process is as follows: weigh the parts of MS medium required for 1L medium to dissolve and mix in sequence; then weigh NAA0.1mg and / or BA0.1mg, respectively added to MS solution to dissolve in turn, constant volume, adjust the pH value to 5.6; finally subpackage, sterilize at high temperature 121℃, high pressure 0.1MPa for 15-20 minutes, cool the culture medium before use.

Embodiment 2

[0031] 1) Primary medium: MS medium + 0.3 mg / L NAA and / or 0.3 mg / L BA, pH 5.8.

[0032] 2) Secondary medium: MS medium + 0.3 mg / L NAA and / or 0.3 mg / L BA + 0.8 mg / L GA, pH 5.8.

[0033] 3) Basic medium: MS medium + 6-BA 0.3mg / L + NAA 0.3mg / L + sucrose 30g / L + agar 7g / L, pH 5.8.

[0034] Taking the configuration of 1L primary medium as an example, the specific preparation process is as follows: Weigh the parts of MS medium required for 1L medium to dissolve and mix in turn; then weigh NAA0.3mg and / or BA0.3mg, respectively added to MS solution to dissolve in turn, constant volume, adjust the pH value to 5.8; finally subpackage, sterilize at high temperature 121°C, high pressure 0.1MPa for 15-20 minutes, cool the culture medium before use.

Embodiment 3

[0036] 1) Primary medium: MS medium + 0.5 mg / L NAA and / or 0.5 mg / L BA, pH 6.0.

[0037] 2) Secondary medium: MS medium + 0.5 mg / L NAA and / or 0.5 mg / L BA + 1.0 mg / L GA, pH 6.0.

[0038] 3) Basic medium: MS medium + 6-BA 0.5 mg / L + NAA 0.5 mg / L + sucrose 32 g / L + agar 8 g / L, pH 6.0.

[0039] Taking the configuration of 1L primary medium as an example, the specific preparation process is as follows: Weigh the parts of MS medium required for 1L medium to dissolve and mix in sequence; then weigh NAA0.5mg and / or BA0.5mg, respectively added to MS solution to dissolve in turn, constant volume, adjust the pH value to 6.0; finally subpackage, sterilize at high temperature of 121°C and high pressure of 0.1MPa for 15-20 minutes, and use the medium after cooling.

[0040] Table 1

[0041]

[0042]It can be seen from Table 1 that the choice of medium components has little effect on tissue culture.

[0043] (2) Rapid propagation and detoxification of grapefruit stem tips

[0044] A m...

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Abstract

The present invention relates to the technical field of detoxification and reproduction of fruit trees, in particular to a method for grafting pomelo micro-buds, comprising the following steps: (1) selection and disinfection of explants, taking pomelo tubers to germinate indoors in spring or autumn, and budding Spray fungicides regularly after growing up, take the buds again two weeks later, put them in 5% sodium hypochlorite solution for 8-10 minutes after heat treatment at 35-40°C, and then put them under the dissecting mirror 8-40 times on the ultra-clean bench Carry out shoot tip stripping, select the shoot tip or nucellus tissue of the terminal bud as a spare; (2) primary culture, put the selected shoot tip or nucellus tissue into 5% sodium hypochlorite solution for 3‑5min, and then put it into the first generation Rapid propagation and growth in the culture medium; the present invention establishes a method for grapefruit microbud grafting through the sterilization of explants, the selection of culture medium and hormones in each stage of tissue culture, and is used for the industrial production of grapefruit tissue culture seedlings. Provide theoretical basis and technical support, and provide a new way for the breeding of new varieties.

Description

technical field [0001] The invention relates to the technical field of micro-bud grafting, in particular to a method for grapefruit micro-bud grafting. Background technique [0002] Pomelo fruit has high nutritional value and sweet taste. It is a very distinctive local characteristic agricultural product. Planting pomelo is of great significance to increase farmers' income and improve people's living standards. [0003] Huanglongbing is a representative disease of pomelo, which has caused the destruction of millions of pomelo orchards in my country. Virus-free treatment of fine varieties is currently the main measure to control these diseases. The stem tip microbud grafting detoxification technology is mainly a rapid propagation and detoxification technology developed by utilizing the principle that the plant shoot apex meristem has no virus and the cell division speed is fast. [0004] Since Murashige et al. proposed micro-grafting technology, it has attracted the attenti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G2/30A01G2/10A01G31/00A01G17/00
CPCA01G2/10A01G2/30A01G17/005A01G31/00A01H4/00A01H4/001A01H4/008
Inventor 张斌袁志辉何福林刘小文刘伟刘春霖
Owner HUNAN UNIV OF SCI & ENG
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