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Method for laying all-male eggs by soft-shelled turtle

A soft-shelled turtle and fertilized egg technology, applied in the direction of animal feed, additional food elements, animal feed, etc., can solve the problem of low reproducibility, and achieve the effects of improving male hatching rate, improving vitality, and promoting secretion

Inactive Publication Date: 2018-08-21
HUNAN UNIV OF ARTS & SCI
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  • Description
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AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is a physical method to artificially induce eggs to hatch all male soft-shelled turtles. The reproducibility of this method is not high, and the proportion of all males is only about 60%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The method that soft-shelled turtle produces whole male egg, comprises the steps:

[0012] (1) Pretreatment of parents: 20 parts of Astragalus membranaceus, 10 parts of C. chinensis, and 8 parts of Cistanche were added to the feed fed every day during the ovulation period and 8 days before the ovulation period of the female parent with mature gonads. The amount of the lure powder prepared from 8 parts, 8 parts, girl fruit, 3 parts red ginseng, and 15 parts donkey-hide gelatin is 5% of the total weight of the feed every day; the father with mature gonads It is necessary to add 10 parts of katydid, 10 parts of sea buckthorn fruit, 10 parts of epimedium, 8 parts of Polygonatum extract, 12 parts of pineapple berry, and 10 parts of scallop powder every day within 5 days before mating. Powder, the addition amount of fortified powder every day is 8% of the total weight of the feed fed every day;

[0013] (2) Fertilized egg treatment: Put the female parent and male parent trea...

Embodiment 2

[0016] The method that soft-shelled turtle produces whole male egg, comprises the steps:

[0017] (1) Parental pretreatment: During the ovulation period and 5 days before the ovulation period of the mature female parent, 15 parts of Astragalus membranaceus, 10 parts of C. 10 parts of fenugreek, 5 parts of girl fruit, 5 parts of red ginseng, and 10 parts of donkey-hide gelatin. In the 5 days before the mating, it is necessary to add 5 parts of kaleidoscope, 10 parts of sea buckthorn fruit, 10 parts of epimedium, 8 parts of Polygonatum extract, 12 parts of pineapple berry and 10 parts of scallop powder every day. powder, the daily supplementary amount of fortified powder is 5% of the total weight of the feed fed every day;

[0018] (2) Put the female parent and male parent treated in step (1) together before ovulation, raise them in a water body with a pH value of 8.5-9.0, and feed them soaked with 5-25PPM human chorionic gonadotropin The content of human chorionic gonadotropi...

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Abstract

The invention discloses a method for laying all-male eggs by a soft-shelled turtle. The method includes pretreatment by traditional Chinese medicine, fertilized egg incubation temperature treatment, water body acidity and alkalinity treatment and the like. According to the method, the parents are pretreated, so that secretion of insulin and sex hormone of the soft-shelled turtle is facilitated, the sperm vitality of the soft-shelled turtle is improved, the expression level of a sex-determining factor Dmrtl of the soft-shelled turtle is improved, the probability of the male fertilized eggs laidby the soft-shelled turtle reaches up to 80% or more, and the incubation temperature of the fertilized eggs is conventionally controlled, so that male hatching rate can be increased by 93% or more.

Description

technical field [0001] The invention relates to the technical field of soft-shelled turtle breeding, in particular to a method for soft-shelled turtles to lay all male eggs. Background technique [0002] Male soft-shelled turtles are large, grow fast, have thick skirts, and less fat in their muscles. The unit price of males is 30% to 50% higher than that of females. Therefore, breeding all-male or Kaohsiung soft-shelled turtles is a difficult problem that must be solved in commercial soft-shelled turtle breeding. In order to overcome the technical difficulties in the study of sex determination in Chinese soft-shelled turtles, Professor Qian Guoying led nearly 10 team members to successfully build an efficient technical platform for the functional analysis of turtle-like genes for the first time, revealing the molecular basis of sex determination and gonad differentiation in Chinese soft-shelled turtles . Through microscopic observation of hundreds of millions of soft-shell...

Claims

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Application Information

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IPC IPC(8): A01K67/02A23K10/30A23K10/26A23K10/22A23K20/184A23K50/00
CPCA01K67/02A23K10/22A23K10/26A23K10/30A23K20/184A23K50/00
Inventor 杨品红张运生王文彬谢中国石彭灵罗丛强
Owner HUNAN UNIV OF ARTS & SCI
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