Microbial preparation for ecological safety water systems and preparation method thereof

A microbial preparation and ecologically safe technology, applied in the field of water treatment, can solve the problems of unfavorable promotion and application, narrow application range, and many compound strains, so as to avoid the loss of bacteria, improve the treatment effect, and facilitate the use and transportation.

Active Publication Date: 2018-08-21
普乐格(厦门)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nitrifying bacteria in this technology are only suitable for low-temperature aquaculture environments such as seawater aquaculture, and the application range is narrow. At the same time, they need to cooperate with corresponding bioreactors. There are many compound strains and cumbersome steps, which is not conducive to the promotion and application in production.

Method used

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  • Microbial preparation for ecological safety water systems and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A kind of microbial preparation provided by the present embodiment, it is prepared according to the following steps:

[0046] (1) Seed production:

[0047] Transfer the Rhodopseudomonas palustris ACCC10649 purchased from the China Agricultural Microorganism Culture Collection and Management Center into the Erlenmeyer flask of the photosynthetic bacteria medium, cultivate it under light at 30°C for 72 hours, and take it out after the medium is turbid .

[0048] The powdered Pichia farinose ACCC20025 purchased from the China Agricultural Microorganism Culture Collection Management Center was transferred into the wort agar I medium, cultured at 28°C, 200 rpm for 48 hours, and taken out after the medium became turbid.

[0049] Transfer Bacillus amyloliquefaciens ACCC06476 purchased from the China Agricultural Microorganism Culture Collection Management Center into nutrient gravy agar medium, shake and culture at 210 rpm at 35°C for 48 hours, and take out the medium after i...

Embodiment 2

[0057] A kind of microbial preparation provided by the present embodiment, it is prepared according to the following steps:

[0058] (1) Seed Production: Prepared according to the method of Example 1.

[0059] (2) Composite strain preparation: according to the following percentage by weight, the strains obtained in step (1) are mixed to obtain composite strains: Rhodopseudomonas palustris 12%, Pichia powdery 28%, Bacillus amyloliquefaciens 22% %, Lactobacillus plantarum 28%, Rhodospirillum exothioni 10%. The composite strains were added to the culture medium at a ratio of 1:100, and cultured in a sealed environment at 30°C for 7 days, and the fermentation broth was obtained after the pH was less than 4.20. Wherein, the culture solution includes: 10 parts by weight of molasses, 15 parts of brown sugar and 90 parts by weight of water.

[0060] (3) Carrier preparation: Concentrate the fermented bacterial liquid obtained in step (2) to a volume of 1 / 4 to obtain concentrated bact...

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Abstract

Relating to the technical field of water treatment, the invention provides a microbial preparation for ecological safety water systems and a preparation method thereof. The microbial preparation comprises: a composite strain composed of 10-20% of rhodopseudomonas palustris, 20-30% of pichia farinose, 20-30% of bacillus amyloliquefaciens, 0-15% of rhodococcus erythropolis, 20-30% of lactobacillus plantarum, and 5-15% of ectothiorhodospira shaposhnikovii; and a carrier for loading the composite strain. The preparation method includes: adding the composite strain into a culture solution, and conducting fermentation culture to obtain a fermentation broth; concentrating the fermentation broth, adding porous starch, conducting stirring adsorption, then adding dextrin and lithium tourmaline micropowder, laying an activated carbon fiber cloth therein to undergo adsorption for 2-3h, and then performing drying. In the microbial preparation, all bacterial strains play a synergistic role to effectively restore the water so as to form a beneficial micro-ecological environment, and the bacterial strains are immobilized on the carrier to effectively improve the microbial purification and restoration effect.

Description

technical field [0001] The invention relates to the technical field of water treatment, and in particular to a microbial preparation for an ecologically safe water system and a preparation method thereof. Background technique [0002] The pollution of water environment in our country is becoming more and more serious. The discharge of untreated aquaculture wastewater and industrial and domestic sewage pollutes the water body, causing serious eutrophication, blue-green algae outbreaks, and a large number of spoilage microorganisms. The ecological environment of aquaculture water bodies or landscape water bodies is severely damaged Therefore, how to restore the water body and build an ecologically safe water system is of great significance. [0003] In the prior art, water restoration mainly includes physical methods, chemical methods and biological methods. Both physical methods and chemical methods have problems such as large amount of engineering, high cost, and incomplete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12N1/16C12R1/38C12R1/84C12R1/07C12R1/01C12R1/25C02F103/20
CPCC02F3/34C02F3/347C02F3/348C02F2103/007C02F2103/20C12N1/16C12N1/20
Inventor 苏清海
Owner 普乐格(厦门)生物科技有限公司
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