Preparation method of esculetin in microsorum fortune and application thereof
A technique of escinolide and star fern, which is applied in the application field of preparing anti-HBV medicines, and can solve the problems such as no reports of escinolide in anti-HBV aspect of escinolide.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0027] Preparation Example
[0028] S1: take the dried stems and leaves of the southern star fern, and crush them;
[0029] S2: Extract with 75% ethanol solution; extract 3 times, the temperature is controlled at 60 degrees.
[0030] S3: When the temperature does not exceed 80 degrees, distill under reduced pressure until the ethanol content of the extract is less than 3%;
[0031] S4: extracting the distilled extract with ethyl acetate;
[0032] S5: After the extraction components are concentrated and dried, they are separated by silica gel column chromatography. First, 2 times the volume of the silica gel is eluted with chloroform, and then 5 times the volume of the silica gel is eluted with a mixed eluent of chloroform / methanol at a ratio of 9:1, and the mixed eluent is collected. The elution component of the stripping agent;
[0033] S6: The eluted fraction of S5 was concentrated and dried, separated by MCl column chromatography, and eluted with 90% methanol solution; ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Granularity | aaaaa | aaaaa |
| Particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More