Wheat for the improvement and prevention of gluten intolerance and wheat dependence-exercise-induced allergic reactions
A technology of wheat and wheat flour, applied in the fields of application, plant genetic improvement, botany equipment and methods, etc., which can solve the problems of research complexity and difficulty
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Embodiment 1
[0059] Embodiment 1: the development of new wheat variety
[0060] A new wheat variety with high cold tolerance and ideal growth habit was developed by artificial crossing Keumkang and Olgeuru to obtain the offspring wheat plant F1. F1 haploids were bred at CIMMYT in Mexico and F1 production was tested in Iksan for four years. The new wheat variety was named "Ofree" ( figure 1 ).
[0061] figure 1 A pedigree diagram illustrating the steps involved in developing the new wheat variety Offree.
Embodiment 2
[0062] Example 2: Genotype identification of Offree
Embodiment 2-1
[0063] Example 2-1: Confirmation of deletion of a gene located at the Glu-B3 locus
[0064] The genetic characterization of Offree developed by the procedure described in Example 1 is based on the genes located at the Glu-1 locus as components of the high molecular weight glutenin subunits (HMW-GSs) in gluten and those located at the Glu-3 locus. Expression levels of genes that are components of the low molecular weight glutenin subunits (LMW-GSs) in gluten were determined.
[0065] Briefly, genomic DNA was extracted from young leaves of Keumkang, Olgeuru and Ofree using the Genomic DNA Extraction Kit for Plants (SolGent Co., Ltd., Korea). Subsequently, polymerization was performed using the genomic DNA extracted earlier as a template and primers capable of detecting genes located at the Glu-A1, Glu-B1, Glu-D1, Glu-A3, Glu-B3, or Glu-D3 loci (listed below) Enzyme chain reaction (PCR) to determine which genes located in the Glu-1 and Glu-3 loci are included in Ofree's geneti...
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