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Buckwheat stem blight resistance identification method

A technology for resistance identification and stem blight, applied in the agricultural field, can solve problems such as restricting the selection and breeding of buckwheat stem blight resistant varieties, and achieve the effects of low cost, high efficiency and accurate resistance.

Active Publication Date: 2018-09-04
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no identification methods of buckwheat stem blight resistance and disease-resistant buckwheat germplasm resources in China, which seriously restricts the breeding of buckwheat stem blight-resistant varieties. Therefore, the current research to establish buckwheat stem blight resistance Identification methods are imminent

Method used

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  • Buckwheat stem blight resistance identification method
  • Buckwheat stem blight resistance identification method
  • Buckwheat stem blight resistance identification method

Examples

Experimental program
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Effect test

Embodiment 1

[0040] This example relates to a specific evaluation example of buckwheat stem blight resistance.

[0041] In the field test, the variety Miqiao-1 with high resistance, the variety ZY12 with medium resistance, the variety SP-7 with moderate sensitivity, and the variety Heiku-5 with high sensitivity were used as test materials. Buckwheat seeds were provided by the Germplasm Resources Preservation Center of Yunnan Academy of Agricultural Sciences.

[0042] Stem blight pathogen strain and its source: the stem blight pathogen Fusarium equiseti strain was purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0043] Specific steps:

[0044] (1) Transplant the pathogenic bacteria of buckwheat stem blight to a PDA medium plate for activation and culture for 6 days, punch holes with a hole punch with a diameter of 10 mm and take a round fungus cake for subsequent use.

[0045] (2) When the buckwheat plant grows to about 50 days, cut off the main stem in the midd...

Embodiment 2

[0067] Using the same method as in Example 1, YZ1, YZ45, Siku No. 1, Yunqiao No. 3, Tianqiao-2, Mihui No. 1, and Mihui No. 2 were identified for stem blight resistance. After one experimenter numbered the healthy plants of 7 different varieties with letters, the plants with only numbers were handed over to another experimenter, and the experiment was carried out according to the steps of method 1. After the experiment is over, check the variety name against the serial number. The results showed that seven different buckwheat varieties showed significant differences in resistance to stem blight, among which YZ1, YZ45, Siku 1, Yunqiao 3, Tianqiao-2, Mihui 1, and Mihui 2 showed significant differences. The results were consistent with the results of actual performance in the field. The verification test of resistance identification confirmed again that the results of resistance identification were consistent with the known actual situation. Therefore, it was verified that this ...

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Abstract

The invention provides a buckwheat stem blight resistance identification method. The method includes the steps of: S1 truncating the main stem of a buckwheat plant, and putting a pathogen round bacterial cake of buckwheat stem blight on a truncated section to obtain an inoculated plant; S2 culturing the inoculated plant in a greenhouse, and observing and recording the lesion expansion situation ofthe plant stem at the end of culture; and S3 performing grading according to the lesion expansion situation, calculating the disease index according to the grade that the lesion expansion situation belongs to, and carrying out resistance evaluation. The buckwheat stem blight resistance identification method provided by the invention only needs direct inoculation of the bacterial cake of pathogento the snipped main stem cut, and has the characteristics of simple operation, rapid onset, low cost and high efficiency. The identification method provided by the invention can accurately evaluate the resistance of buckwheat.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for identifying resistance to stem blight of plants. Background technique [0002] Buckwheat stem blight is a serious fungal disease caused by Fusarium equiseti, which is common in buckwheat planting areas in my country, and the plant incidence rate can be as high as 30-50% when the disease occurs seriously. In the early stage of plant disease, sporadic brown lesions can be seen on the stem nodes. As the disease progresses, the brown lesions expand along the stem, gradually expanding to damage branches, leaves, and seeds. Harm the whole plant. During drought, leaves, branches, and main stems wither and droop, and the whole plant withers and dies, resulting in a serious decline in buckwheat production and serious economic losses for buckwheat production. At present, buckwheat stem blight has become one of the main diseases seriously affecting buckwheat p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/77
CPCC12Q1/18G01N2333/37
Inventor 卢文洁王莉花孙道旺王艳青尹桂芳李春花
Owner 云南省农业科学院生物技术与种质资源研究所