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Complete biological artificial intervertebral disk and preparation method thereof

An intervertebral disc, complete technology, used in tissue regeneration, spinal implants, medical science and other directions, can solve problems such as not involving intervertebral discs, and achieve the effect of improved compressive strength and high mechanical properties

Pending Publication Date: 2018-09-07
南京邦鼎生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these studies continue to promote the development of tissue engineering regeneration of intervertebral discs, none of them involve the tissue engineering regeneration of complete intervertebral discs.

Method used

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  • Complete biological artificial intervertebral disk and preparation method thereof
  • Complete biological artificial intervertebral disk and preparation method thereof
  • Complete biological artificial intervertebral disk and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: Tissue engineering bioscaffold of regenerable intervertebral disc

[0044] A tissue engineered biological scaffold for regenerating intervertebral disc, its structure is as follows figure 1 As shown, it includes a nucleus pulposus support 1, annulus fibrosus support 2, an endplate support 3 and a peripheral elastic support ring 4; the nucleus pulposus support 1 is located at the center of the entire biological support system, and the annulus fibrosus support 2 is arranged around the nucleus pulposus support 1 in the circumferential direction. The plate support 3 is arranged axially along the fibrous annulus support 2 and covers both ends of the fibrous annulus support 2 , and the peripheral elastic support ring 4 circumferentially covers the fibrous annulus support 2 and the endplate support 3 .

[0045] The nucleus pulposus scaffold 1 is chitosan polyethylene glycol cross-linked hydrogel.

[0046] The fiber ring support 2 is a multi-layer structure compo...

Embodiment 2

[0060] Embodiment 2: Tissue engineering bioscaffold of regenerable intervertebral disc

[0061] A tissue engineered biological scaffold for regenerating intervertebral disc, its structure is as follows figure 1 As shown, it includes a nucleus pulposus support 1, annulus fibrosus support 2, an endplate support 3 and a peripheral elastic support ring 4; the nucleus pulposus support 1 is located at the center of the entire biological support system, and the annulus fibrosus support 2 is arranged around the nucleus pulposus support 1 in the circumferential direction. The plate support 3 is arranged axially along the fibrous annulus support 2 and covers both ends of the fibrous annulus support 2 , and the peripheral elastic support ring 4 circumferentially covers the fibrous annulus support 2 and the endplate support 3 .

[0062] The nucleus pulposus scaffold 1 is sodium alginate hydrogel.

[0063] The fiber ring support 2 is a multi-layer structure composed of PBSA elastic porous...

Embodiment 3

[0077] Example 3: Tissue Engineering Biological Scaffold Inoculated with Seed Cells

[0078]Inoculate mesenchymal stem cells (MSC) in the hydrogel (nucleus pulposus support), fibrous annulus support and porous endplate support prepared in steps 1, 3 and 4 of Example 2, at a seeding density of about 6-10 × 10 6 After inoculation, different growth factors were added to induce differentiation, and then each component unit of the loaded cells was assembled to obtain a bioscaffold that could regenerate the whole intervertebral disc.

[0079] The resulting bioscaffold inoculated with seed cells can either be cultured in vitro to regenerate the entire intervertebral disc and then transplanted into the body, or it can be transplanted into the body first to complete the regeneration in a real environment.

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Abstract

The invention provides a tissue engineering biological stent system for regenerating complete intervertebral disks. The system comprises a nucleus pulposus stent, an annulus fibrosus stent, end platestents and a peripheral elastic support ring; the nucleus pulposus stent is located at the center of the whole biological stent system, the annulus fibrosus stent is circumferentially arranged aroundthe nucleus nucleus stent, the end plate stents are arranged in the axial direction of the annulus fibrosus stent and cover the two ends of the annulus fibrosus stent, and the peripheral elastic support ring circumferentially wraps the annulus fibrosus stent and the end plate stents; the nucleus pulposus stent is made of biodegradable hydrogel; the annulus fibrosus stent is of a multi-layer structure formed by coaxially winding biodegradable elastic membranes; the end plate stents are made of a biodegradable porous material; the peripheral elastic support ring is a biodegradable non-porous elastic ring. The invention further provides a complete biological artificial intervertebral disk regenerated based on the biological stent system and a preparation method thereof. The biological artificial intervertebral disk has a complete mammalian intervertebral disk structure and has biomechanical properties close to those of a natural intervertebral disk.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a tissue engineering method and a structural system for total intervertebral disc regeneration. Background technique [0002] The main cause of low back pain comes from degeneration of intervertebral discs. Clinically, it is necessary to surgically remove the ruptured annulus of intervertebral discs to prevent pain from compression of peripheral nerves. Disc fusion using metal grafts is the preferred method. But it reduces the flexibility of the spine and accelerates the degeneration of adjacent intervertebral discs. [0003] Tissue engineering has been used in the research of intervertebral disc regeneration in recent years, but most tissue engineering methods aim at a part of the intervertebral disc, such as the annulus fibrosus (AF) or nucleus pulposus (NP), or a combination of the two. In terms of research on AF, Wan In Biomaterials 2008, 29, 643-652, Y. et al. used dec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/38A61L27/20A61L27/18A61F2/44
CPCA61L27/18A61L27/20A61L27/3834A61L27/3856A61L27/50A61F2/442A61F2002/4445A61L2430/38C08L5/04C08L67/04
Inventor 万玉青
Owner 南京邦鼎生物科技有限公司
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