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Fluorine-nitrogen doped carbon quantum dot as well as preparation method and application thereof

A carbon quantum dot, nitrogen doping technology, applied in the fields of nano-biomedical materials, synthesis technology and biomaterials, can solve the problems of narrow cell range, restricted wide application, etc., and achieves simple method, good biocompatibility, and cytotoxicity. low effect

Active Publication Date: 2018-09-07
NANJING WISEGEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cell transfection efficiency of the above-mentioned quantum dots is only equivalent to that of 25kDa PEI, and the range of applicable cells is narrow, thus restricting its wide application in clinical medicine

Method used

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  • Fluorine-nitrogen doped carbon quantum dot as well as preparation method and application thereof
  • Fluorine-nitrogen doped carbon quantum dot as well as preparation method and application thereof
  • Fluorine-nitrogen doped carbon quantum dot as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] With 2,3,5,6-tetrafluoroterephthalic acid as the fluorine source, then add 1.8kDa BPEI as the nitrogen source, dissolve in ethanol to obtain the precursor solution (2,3,5,6-tetrafluoro-p-phenylene Diformic acid concentration is 70mmol / L, 1.8kDa BPEI concentration is 35mmol / L), then the precursor solution is transferred to a polytetrafluoroethylene autoclave and reacted at 180°C for 6h, cooled to room temperature to obtain a suspension, Separation by dialysis and lyophilization to obtain fluorine and nitrogen doped carbon quantum dots (F-CDs).

[0045] figure 1 It is a TEM image of F-CDs. It can be seen that the size of fluorine and nitrogen doped carbon quantum dots is uniform, about 5nm, and the dispersion is uniform. figure 2 It is the XPS graph of F-CDs. It can be seen that there are 4 signals in the graph, which are 284.8eV (C 1s), 398.4eV (N 1s), 531.6eV (O 1s) and 686.8eV (F 1s).

[0046] The 19F NMR method is used to characterize and detect the above products....

Embodiment 2

[0051] With 2,3,5,6-tetrafluoroterephthalic acid as fluorine source, add 600Da BPEI as nitrogen source, dissolve in ethanol, obtain precursor solution (2,3,5,6-tetrafluoroterephthalic acid Concentration is 70mmol / L, 600Da BPEI concentration is 35mmol / L), and then the precursor solution is transferred to a polytetrafluoroethylene autoclave and reacted at 180°C for 6h, cooled to room temperature to obtain a suspension, and then dialyzed Separation and freeze-drying to obtain fluorine- and nitrogen-doped carbon quantum dots. Refer to Example 1 for gene transfection efficiency experiments and cytotoxicity experiments. The gene transfection vector prepared in this example has the best transfection efficiency (81.21%) of HEK 293T cells and the transfection efficiency (64.24%) of COS-7 cells, far higher than the transfection efficiency of 600Da BPEI in HEK 293T cells Transfection efficiency (1.35%) and COS-7 cell transfection efficiency (0.35%), also significantly higher than 25kDa ...

Embodiment 3

[0053] With 2,3,5,6-tetrafluoroterephthalic acid as fluorine source, then add 25kDa BPEI as nitrogen source, dissolve in ethanol, obtain precursor solution (2,3,5,6-tetrafluoroterephthalic acid The concentration is 70mmol / L, and the concentration of 25kDa BPEI is 35mmol / L), then the precursor solution is transferred to a polytetrafluoroethylene autoclave and reacted at 180°C for 6h, cooled to room temperature to obtain a suspension, and then dialyzed Separation and freeze-drying to obtain fluorine- and nitrogen-doped carbon quantum dots. Refer to Example 1 for gene transfection efficiency experiments and cytotoxicity experiments. The best transfection efficiency (85.11%) of HEK 293T cells and the transfection efficiency (68.29%) of COS-7 cells of the gene transfection vector prepared in this example are obviously higher than 25kDa PEI (HEK 293T~56.25%, COS-7~22.66%) and Lipo 2000 (HEK 293T~65.11%, COS-7~33.47%) efficiency, and the average fluorescence intensity of transfected...

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Abstract

The invention provides a fluorine-nitrogen doped carbon quantum dot prepared from a fluorine-containing aromatic compound and a cationic polymer. The fluorine-containing aromatic compound is linked tothe cationic polymer through a covalent bond; and the cationic polymer is linear polyethyleneimine or branched polyethyleneimine. The invention also provides a preparation method of the fluorine-nitrogen doped carbon quantum dot and application of the fluorine-nitrogen doped carbon quantum dot as a gene delivery vector. The preparation method provided by the invention adopts a hot solvent to prepare the fluorine-nitrogen doped carbon quantum dot with a one-pot method, is simple in reaction, low in preparation cost, available in raw material and high in yield, can reach the efficient transfection effect in the cell transfection process, is relatively small in toxicity produced to cells in the transfection process, can effectively and safely deliver gene molecules into the cells and is a gene transfection vector which has the advantages of high efficiency, low toxicity, low cost, simpleness and easiness in synthesis and the like.

Description

technical field [0001] The invention relates to the field of nano-biomedical materials, more particularly to the technical fields of synthesis technology and biological materials, in particular to a method for preparing fluorine- and nitrogen-doped carbon quantum dots from fluorine-containing aromatic compounds and cationic polymers, and the carbon quantum dots Applications in gene delivery. Background technique [0002] Gene therapy refers to the transient expression or function of exogenous gene fragments in the host cell, or the integration of exogenous gene fragments into the host cell chromosome to make it stably expressed, thereby repairing or supplementing the function of the host cell. Successful gene therapy is inseparable from efficient and safe gene vectors. According to needs, people have designed and prepared a variety of gene vectors, which can be mainly divided into viral vectors and non-viral vectors. At present, viral vectors can achieve high-efficiency tra...

Claims

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Application Information

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IPC IPC(8): C01B32/15C12N15/87
CPCC12N15/87C01B32/15
Inventor 董伟魏云珍董奕汐
Owner NANJING WISEGEN BIOTECH
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