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Application of system for detecting ORM1 (orosomucoid 1) gene expression quantity for diagnosing tuberculosis

A gene expression, tuberculosis technology, applied in the application field of tuberculosis diagnosis, can solve the problems of no ORM1, inability to effectively distinguish active tuberculosis and latent infection, and high false-negative tuberculosis diagnosis

Inactive Publication Date: 2018-09-07
中国人民解放军第三0九医院
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AI Technical Summary

Problems solved by technology

Imaging diagnosis is difficult to distinguish pulmonary tuberculosis from other lung diseases; false negatives in tuberculosis diagnosis are high; immunological diagnosis mainly includes antibody detection and cellular immune detection, and serological detection has low specificity. PPD skin test is currently the most commonly used tuberculosis infection IGRA is currently the best cellular immunological test, but it cannot effectively distinguish between active tuberculosis and latent infection
[0005] Serum mucin ORM1 (orosomucoid 1), also known as α-acid glycoprotein 1, is mainly secreted and expressed by liver cells, and is also synthesized by endothelial cells and tumor cells. It can be used as a marker for tumor diagnosis and efficacy evaluation, but currently there is no ORM1 for the diagnosis of tuberculosis logo coverage

Method used

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  • Application of system for detecting ORM1 (orosomucoid 1) gene expression quantity for diagnosing tuberculosis
  • Application of system for detecting ORM1 (orosomucoid 1) gene expression quantity for diagnosing tuberculosis
  • Application of system for detecting ORM1 (orosomucoid 1) gene expression quantity for diagnosing tuberculosis

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Embodiment 1

[0040] Example 1, the expression level of ORM1 gene can be used to distinguish tuberculosis patients from healthy people

[0041] 1. Research object

[0042] Tuberculosis group: 16 patients with active pulmonary tuberculosis. The inclusion criteria for this group were positive acid-fast staining of sputum smears, no diabetes, tumors and immune-related diseases, and no immunosuppressants were used.

[0043] Healthy control group: 16 healthy people of the same gender and age as the tuberculosis group. The inclusion criteria for this group were no fever, cough and other symptoms, and no active lesions on chest X-ray.

[0044] 2. Detection of ORM1 expression in peripheral blood

[0045] 1. Collect the peripheral blood of each subject in the tuberculosis group and the healthy control group.

[0046] 2. Isolation of peripheral blood mononuclear cells (PBMCs): Dilute 2ml of collected peripheral blood with basal medium 1640 medium, and then use Ficoll (GE Biosciences, USA) for densi...

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Abstract

The invention discloses an application of system for detecting an ORM1 (orosomucoid 1) gene expression quantity for diagnosing tuberculosis. The application discovers that the ORM1 gene expression quantity has an obvious difference in tuberculosis patients and healthy people; an analysis result of a subject job characteristic curve indicates that the ORM1 gene expression quantity is used for distinguishing that the tuberculosis patients and the healthy people have high sensitivity and specificity, which explains that the tuberculosis can be diagnosed through the detection of the ORM1 gene expression quantity of an object to be detected.

Description

technical field [0001] The invention relates to the application of a system for detecting the expression of ORM1 gene in the diagnosis of tuberculosis in the field of biotechnology. Background technique [0002] Tuberculosis is a worldwide infectious disease that seriously threatens human health. According to the World Health Organization (WHO), there were 10.4 million new tuberculosis patients and 1.67 million deaths in 2016. It is the most deadly infectious disease. [0003] There are many reasons for the prevalence of tuberculosis, among which the lack of specific and effective diagnostic techniques for active tuberculosis is crucial. Failure to diagnose early will lead to delayed treatment, increased treatment costs and mortality on the one hand; on the other hand, the source of infection cannot be effectively controlled, resulting in the spread of tuberculosis. Therefore, the development of specific and effective diagnostic reagents for active tuberculosis is of great ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158
Inventor 程小星杨秉芬翟斐
Owner 中国人民解放军第三0九医院
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