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Pretreatment kit for salbutamol sample based on immunomagnetic beads

A technology of immunomagnetic beads and salbutamol, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of complicated purification and separation operations, low separation efficiency, etc., to reduce the interference of background substances, improve accuracy and precision, and improve the operation easy effect

Pending Publication Date: 2018-09-07
沭阳康源泰博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to make up for the deficiencies in the prior art, and to provide a salbutamol sample pretreatment kit for immunomagnetic beads, which is used for the enrichment and purification of salbutamol in the sample, so as to solve the complicated purification and separation operation of the salbutamol sample sample. Low separation efficiency and other technical problems

Method used

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  • Pretreatment kit for salbutamol sample based on immunomagnetic beads

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Effect test

Embodiment 1

[0032] 1. Preparation of anti-albuterol antibody immunomagnetic beads:

[0033] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the magnetic beads, repeat three times, each time with an interval of 3 minutes, remove the activation buffer, add 200 μL of activator EDC and NHS each, mix well, and incubate at 37°C for 2 hours , to activate the magnetic beads;

[0034] 2. Wash the magnetic beads with coupling buffer three times, with an interval of 3 minutes each time, add salbutamol antibody, mix well, and incubate at 37°C for 3 hours to prepare salbutamol antibody immunomagnetic beads;

[0035] 3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 3 minutes between each time. Incubate with blocking solution at 37°C for 2 hours, store in storage buffer at 4°C until use;

[0036] 2. The application of anti-salbutamol immunomagnetic beads to the enrichment of salbutamol in animal tissue samples:

[0037] 1. Grind ...

Embodiment 2

[0042] 1. Preparation of anti-albuterol antibody immunomagnetic beads:

[0043] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the magnetic beads, repeat three times with an interval of 5 minutes each time, remove the activation buffer, add 180 μL of activator EDC and NHS each, mix well, and incubate at 37°C for 2 hours , to activate the magnetic beads;

[0044] 2. Wash the magnetic beads with coupling buffer three times, with an interval of 3 minutes each time, add salbutamol antibody, mix well, and incubate at 37°C for 3 hours to prepare salbutamol antibody immunomagnetic beads;

[0045]3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 3 minutes between each time. Incubate with blocking solution at 37°C for 2 hours, store in storage buffer at 4°C until use;

[0046] 2. Application of anti-salbutamol immunomagnetic beads to the enrichment of salbutamol in feed samples:

[0047] 1. Weigh 2.0 g of crushe...

Embodiment 3

[0050] 1. Preparation of anti-albuterol antibody immunomagnetic beads:

[0051] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the magnetic beads, repeat three times, each time at an interval of 4 minutes, remove the activation buffer, add 250 μL of activator EDC and NHS each, mix well, and incubate at 37°C for 4 hours , to activate the magnetic beads;

[0052] 2. Wash the magnetic beads with coupling buffer three times, with an interval of 4 minutes each time, add salbutamol antibody, mix well, and incubate at 37°C for 4 hours to prepare salbutamol antibody immunomagnetic beads;

[0053] 3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 4 minutes between each time. Incubate with blocking solution at 37°C for 4 hours, store in storage buffer at 4°C until use;

[0054] 2. The application of anti-salbutamol immunomagnetic beads to the enrichment of salbutamol in samples:

[0055] 1. Grind the animal tissu...

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Abstract

The invention provides preparation and an application of a pretreatment kit for a salbutamol sample based on immunomagnetic beads. The kit mainly comprises a sample bin, an agent bin, a cleaning bin,an elution bin and the like. By virtue of enrichment of the immunomagnetic beads and adequate dispersion of the immunomagnetic beads in liquid, the bonding area is enlarged, the immunoreactions is relatively thorough, and the kit can be widely applied to the enrichment an separation of salbutamol in products such as animal tissues, aquatic products and meat products, so that the detection sensitivity can be improved, and the detection leaking phenomenon is avoided; and meanwhile, the use of a large number of organic solvent is reduced.

Description

technical field [0001] The invention relates to a salbutamol sample pretreatment kit based on immunomagnetic beads. Background technique [0002] Salbutamol is a synthetic β-adrenaline stimulant. It is used as clenbuterol in modern animal husbandry to accelerate animal growth and increase the lean meat rate of fat animals. However, excessive intake of salbutamol will cause extremely serious damage to the human body. It is prone to symptoms such as muscle tremors, headaches, nausea, and vomiting, especially for patients with diseases such as high blood pressure, heart disease, hyperthyroidism, and prostatic hyperplasia. Severe cases can lead to death. In 2002, my country banned the use of salbutamol in animal husbandry. [0003] Due to the complexity of biological components, low concentration of analytes, complex pretreatment process and other factors, the existing albuterol extraction process is not stable, resulting in low enrichment efficiency of extracted albuterol, whi...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/531
CPCG01N33/531G01N33/54326
Inventor 张波张学记郗日沫王鹏雷少军张二林雷达刘向阳孙骁帅
Owner 沭阳康源泰博生物科技有限公司
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