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A method and product thereof for preparing soybean 7s protein and soybean protein hydrolyzate

A technology of soybean protein and hydrolyzate, which is applied in the direction of protein food ingredients, protein food processing, plant protein processing, etc., can solve the problems that it is difficult to meet different needs, the functional characteristics of soybean protein isolate are not outstanding, and achieve emulsification and foaming properties Improved effect

Active Publication Date: 2021-04-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The functional properties of natural soybean protein isolate are not outstanding, making it difficult to meet the different needs of food systems for protein functional properties

Method used

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  • A method and product thereof for preparing soybean 7s protein and soybean protein hydrolyzate
  • A method and product thereof for preparing soybean 7s protein and soybean protein hydrolyzate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]The degreased bean powder was dispersed in deionized water (1:10), and stirred with stirring, and the alkali was adjusted pH 8.0, stirred at room temperature for 2 h, and the precipitate was removed at 6500 rpm. The supernatant regulates pH 4.5, still 30 min, At 3750 rpm, it was centrifuged to precipitate in deionized water, and the pH 7 was adjusted for 2-3 hours. The protein concentration after reconstallization was adjusted to 7%, and the protein solution was heated at 65 ° C for 10 min (at a solution center thermometer). The protein solution was cooled to 37 ° C, and 1% glutathione and soy lecithin were added, pH was adjusted to 2, adding gastric protease, hydrolysis for 3 hours, and adjusted pH to 7, and precipitate at 6500 rpm. The resulting supernatant was treated at 130 ° C for 4 to 8 s, and the enzyme sterilization was performed. After spray drying, it was 7S and hydrolyzed by soy protein. The spray drying process conditions are from 190 ° C in the air, the air temper...

Embodiment 2

[0035]The degreased bean powder was dispersed in deionized water (1:10), and stirred with stirring, and the alkali was adjusted pH 8.0, stirred at room temperature for 2 h, and the precipitate was removed at 6500 rpm. The supernatant regulates pH 4.5, still 30 min, At 3750 rpm, it was centrifuged to precipitate in deionized water, and the pH 7 was adjusted for 2-3 hours. The protein concentration after reconstallization was adjusted to 7%, and the protein solution was heated at 65 ° C for 10 min (at a solution center thermometer). The protein solution was cooled to 37 ° C, and 1% glutathione, pH was adjusted to 2, adding gastric protease 1%, hydrolysis for 3 hours, and adjusted pH to 7, and precipitated at 6500 rpm. The resulting supernatant was treated at 130 ° C for 4 to 8 s, and the enzyme sterilization was performed. After spray drying, it was 7S and hydrolyzed by soy protein. The spray drying process conditions are from 190 ° C in the air, the air temperature is 80 ° C, and the...

Embodiment 3

[0038]The degreased bean powder was dispersed in deionized water (1:10), and stirred with stirring, and the alkali was adjusted pH 8.0, stirred at room temperature for 2 h, and the precipitate was removed at 6500 rpm. The supernatant regulates pH 4.5, still 30 min, At 3750 rpm, it was centrifuged to precipitate in deionized water, and the pH 7 was adjusted for 2-3 hours. The protein concentration after reconstallization was adjusted to 7%, and the protein solution was heated at 65 ° C for 10 min (at a solution center thermometer). The protein solution was cooled to 37 ° C, 1% soy lecithin, pH adjusted to 2, adding gastric protease, hydrolysis for 3 hours, and adjusted pH to 7, and precipitate at 6500 rpm. The resulting supernatant was stem bacteria, which was 7S and hydrolyzed by soy protein after spray drying. The spray drying process conditions are from 190 ° C in the air, the air temperature is 80 ° C, and the liquid flow rate is 18 rpm.

[0039]7S and hydrolyzate in the soy protein...

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Abstract

The invention discloses a method for preparing soybean 7S protein and soybean protein hydrolyzate and products thereof. It includes separation, heating, enzymatic hydrolysis, enzyme inactivation and drying. The heating method of the present invention and the method of adding glutathione and soybean lecithin before enzymolysis work together to open the disulfide bond and hydrophobic bond of the protein structure, so that the enzymolysis can be carried out more fully, which is beneficial to the unfolding of the 11s protein , which is conducive to the selective hydrolysis of 11s protein by pepsin. The method for producing 7s protein and its hydrolyzate provided by the present invention increases the yield of soybean protein by about 15 percentage points compared with the traditional technology, and the degree of protein hydrolysis reaches more than 20%. , The solubility, emulsification and foaming properties of the product have been greatly improved, the foaming property is above 190%, the foaming stability reaches 93.9%, and the yield reaches above 55%.

Description

Technical field[0001]The present invention belongs to the field of soy protein extraction, and in particular, the present invention relates to a method of preparing soybean 7S protein and soy protein hydrolyzate and its products.Background technique[0002]Natural soy separation protein functionality is not highlighted, making it difficult to meet the different needs of the food system on protein functionality. To this end, it is necessary to use some ways to improve the functional characteristics of soy separation proteins, enabling them to be applied to a wide variety of food systems.Inventive content[0003]The purpose of this section is to summarize some of the preferred embodiments of the embodiments of the present invention and some preferred embodiments. Some simplifications or omitted in this section and the present invention may be simplified or omitted to avoid blurring the purpose of this section, the description, and the present invention, and this simplifies or omitted cann...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/16A23J3/34
CPCA23J3/16A23J3/346
Inventor 陈洁梁贵江何志勇曾茂茂秦昉高大明张爽陶冠军王林祥
Owner JIANGNAN UNIV