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Rapid detection method for nitrate and nitrite in fruits and vegetables and meat products

A technology for nitrite and nitrite, applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc., can solve problems such as interference, inability to eliminate measurement, difficulty in nitrate and nitrite detection, etc., and achieve high recovery rate , low cost and simple sample preparation

Inactive Publication Date: 2018-09-14
TIANJIN AGRICULTURE COLLEGE
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  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the difficulty of detecting nitrate and nitrite in samples with high salt content by using high-efficiency capillary electrophoresis, the present invention cannot eliminate Cl - to NO 2 - The problem of interference caused by the determination of the test provides a method for the rapid detection of nitrate and nitrite in fruits, vegetables and meat products

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  • Rapid detection method for nitrate and nitrite in fruits and vegetables and meat products
  • Rapid detection method for nitrate and nitrite in fruits and vegetables and meat products
  • Rapid detection method for nitrate and nitrite in fruits and vegetables and meat products

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Embodiment 1

[0024] Embodiment 1: A method for rapidly detecting nitrate and nitrite in fruits, vegetables and meat products, using high-efficiency capillary electrophoresis-direct ultraviolet method to simultaneously detect the content of nitrate and nitrite in the sample, the specific steps are as follows:

[0025] (1) Sample pretreatment: Fresh celery purchased from the market was used as the sample to be tested, and it was pulverized and homogenized by a blender for later use. Take 1 g of crushed and homogenized sample, add about 5 mL of ultrapure water, bathe in a constant temperature water bath at 50 °C for 15 min, take it out and cool to room temperature, dilute to 10 mL with ultrapure water, filter with filter paper, and the filtrate Filter with a 0.22 µm pore size filter and then go on the machine (the dilution factor of the sample can be adjusted appropriately).

[0026] (2) Detection conditions of high performance capillary electrophoresis-direct ultraviolet method: electrophore...

Embodiment 2

[0035] Embodiment 2: A method for rapidly detecting nitrate and nitrite in fruits, vegetables and meat products, using high-efficiency capillary electrophoresis-direct ultraviolet method to simultaneously detect the content of nitrate and nitrite in the sample, the specific steps are as follows:

[0036] Sample pretreatment: The homemade sausage was used as the sample to be tested, and it was pulverized and homogenized by a mixer for later use. Take 1 g of crushed and homogenized sample, add about 5 mL of ultrapure water, bathe in a constant temperature water bath at 50 °C for 15 min, take it out and cool to room temperature, dilute to 10 mL with ultrapure water, filter with filter paper, and the filtrate Filter with a 0.22 µm pore size filter and then go on the machine (the dilution factor of the sample can be adjusted appropriately).

[0037] High-efficiency capillary electrophoresis-direct ultraviolet method detection: separation voltage-4KV, separation buffer: final concen...

Embodiment 3

[0040] Embodiment 3: A kind of method for rapid detection of nitrate and nitrite in fruits and vegetables and meat products, adopts high-efficiency capillary electrophoresis-direct ultraviolet method to detect the content of nitrate and nitrite in the sample simultaneously, concrete steps are as follows:

[0041] Sample pretreatment: Harbin red sausage was used as the sample to be tested, and it was pulverized and homogenized by a mixer for later use. Take 1 g of crushed and homogenized sample, add about 5 mL of ultrapure water, bathe in a constant temperature water bath at 50 °C for 15 min, take it out and cool to room temperature, dilute to 10 mL with ultrapure water, filter with filter paper, and the filtrate Filter with a 0.22 µm pore size filter and then go on the machine (the dilution factor of the sample can be adjusted appropriately).

[0042] High-efficiency capillary electrophoresis-direct ultraviolet method detection: separation voltage-7KV, separation buffer: final...

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Abstract

Belonging to the technical field of food safety detection, in order to solve the detection difficulty of nitrate and nitrite in high-salt content samples at present and eliminate the interference of Cl<-> to NO2<-> determination, the invention provides a rapid detection method for nitrate and nitrite in fruits and vegetables and meat products. The method includes: detecting the content of nitrateand nitrite in a sample by high performance capillary electrophoresis-direct ultraviolet method simultaneously, conducting sample pretreatment, making a standard curve linear regression equation according to high performance capillary electrophoresis-direct ultraviolet method, acquiring the recovery of standard addition, calculating a lowest detection limit, and analyzing the data to obtain a detection result of the to-be-detected sample. The method has the characteristics of simple sample preparation, fast separation speed, strong selectivity, and small sample consumption, and at the same time eliminates the interference of Cl<-> in the determination process. The method is not limited to low-salt content samples, also can determine the common high salt content samples which easily cause food poisoning due to high content of nitrate and nitrite, like pickles, salted meat and the like, and has strong practical significance.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a method for rapidly detecting nitrate and nitrite in fruits, vegetables and meat products. Background technique [0002] Nitrate and nitrite are closely related to human life. They are often used in cured meat products, which have antiseptic and color development effects. Vegetables, as the main source of daily vitamins and cellulose for the human body, contain more nitrate, and nitric acid Salt will be reduced to nitrite under certain conditions. After a large amount of nitrite enters the human body, it will convert the oxygen-carrying myoglobin into metmyoglobin, causing hypoxia in the human body tissue, causing intestinal cyanosis, causing acute poisoning, and also combining with amines in the human body The generation of carcinogens such as nitrosamines brings good color and shelf life to food, but also brings harm to human health. According to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 马俪珍李秀明李双燕吴晨燕杨华梁丽雅
Owner TIANJIN AGRICULTURE COLLEGE
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