Drug-loaded liquid metal compound, and preparation method and application thereof

A liquid metal and composite technology, applied in the field of medicine, can solve problems such as the negative effects of nano-drugs, and achieve the effects of promoting drug release, good injectability, and simple preparation method

Inactive Publication Date: 2018-09-18
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the potential negative impact of nanome

Method used

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  • Drug-loaded liquid metal compound, and preparation method and application thereof
  • Drug-loaded liquid metal compound, and preparation method and application thereof
  • Drug-loaded liquid metal compound, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Toxic side effects and biocompatibility of liquid metals

[0025] 1. Cell experiments

[0026] The cell verification experiment was carried out in accordance with the national standard GB / T16886.5 of the People's Republic of China (Medical Device Biological Evaluation Part 5: In vitro Cytotoxicity Test) and the relevant provisions of the International Medical Device Biological Evaluation Standard ISO10993-5, using mouse fibroblast L929 And human breast cancer cell MCF-7 as the research object, the in vitro biosafety evaluation of liquid metal was carried out.

[0027] Take 1mL of liquid metal for two days of ultraviolet sterilization, add 200μL of liquid metal to a 5ml EP tube, oscillate to make the liquid metal adhere to the tube wall and cover the entire bottom of the tube, remove excess liquid metal, add RPMI1640 basic culture medium, and make the liquid metal and liquid The surface area to volume ratio is 3 cm 2 / mL at 37°C, 5% CO 2 After 24 hours in t...

Embodiment 2

[0031] Embodiment 2: Preparation of drug-loaded liquid metal complex

[0032] The preparation process of the drug-loaded liquid metal complex is as follows: figure 1 As shown, it specifically includes the following steps:

[0033] (1) Preparation of drug-loaded silica: Prepare silica by template method, weigh 0.417g CTAB and 4.69g dodecylamine and add to a mixed solution of 180mL pure water, 300mL absolute ethanol and 20mL isopropanol, then Add 0.07mL of ammonia water and 15mL of tetraethyl orthosilicate, stir at 300r / min for 15min, then stir for 2h after observing that the solution becomes turbid, wash with ethanol for 3 times, dry or freeze-dry, sinter at 550K for 4h to remove the template agent, and obtain two silicon oxide. Take 20 mg of silicon dioxide and add it to a doxorubicin solution with a concentration of 2 mg / ml, and centrifuge to collect the lower precipitate to obtain drug-loaded silicon dioxide.

[0034] (2) Add 1 mL of liquid metal to the EP tube, then add ...

Embodiment 3

[0036] Embodiment 3: Preparation of drug-loaded liquid metal complex

[0037] (1) The difference from Example 2 is that 60 mg of silicon dioxide is added to a paclitaxel solution with a concentration of 2 mg / ml, and the solvent is volatilized to obtain drug-loaded silicon dioxide;

[0038] (2) The difference from Example 2 is that the surfactant is sorbitan, and 100 μL of sorbitan is added to 1 ml of liquid metal;

[0039] (3) Add 1 mL of liquid metal pellets to 60 mg of drug-loaded silica, and mix for 30 seconds at room temperature using a vortex mixer to obtain the drug-loaded liquid metal complex.

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Abstract

The invention discloses a drug-loaded liquid metal compound, and a preparation method and an application thereof, and belongs to the technical field of medicines. The preparation method comprises thefollowing steps: mixing liquid metal with a surfactant, stopping mixing when the liquid metal becomes liquid metal pellets, and removing the excess surfactant; and mixing a medicinal preparation or adrug-loaded micro-nano preparation with the liquid metal pellets to obtain the drug-loaded liquid metal compound. The liquid metal is a liquid at normal temperature, and has good injectability, excellent biocompatibility, excellent CT imaging function and good magnetocaloric effect. The in-situ multi-point injection of the drug-loaded liquid metal compound is performed under the guidance of CT imaging to make the compound uniformly distributed in the tumor site, the tumor undergoes uniform thermal stimulus under the action of a certain size of alternating magnetic field, and the vortex heat generating effect of the liquid metal makes the temperature of the tumor kept at 43-48 DEG C in order to achieve magnetic hyperthermia of the tumor; and heat can stimulate the sensitivity of tumor cellsto the drug, promote drug release and achieve chemotherapy and magnetic hyperthermia combined treatment of tumors.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a drug-loaded liquid metal compound and its preparation method and application. Background technique [0002] Cancer is a major disease that threatens human health. The treatment of malignant tumors has always been a major problem in the medical field, and it is also a research hotspot. The treatment methods have gradually developed from surgical treatment to various treatment methods. Common methods of tumor treatment include surgical treatment, chemotherapy, radiotherapy, hyperthermia, etc. Surgical treatment is the most common treatment method, but the tumor has the characteristics of infiltrating growth and unlimited proliferation, it is difficult to completely remove it by surgery, and recurrence after surgery often occurs Condition. Chemotherapy and radiotherapy are often combined with surgery to improve the survival rate of cancer patients. However, chemothe...

Claims

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Application Information

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IPC IPC(8): A61K47/04A61K47/02A61K45/00A61K41/00A61P35/00A61K31/337A61K31/704A61K31/4745
CPCA61K31/337A61K31/4745A61K31/704A61K41/0052A61K45/00A61K47/02A61P35/00A61K2300/00
Inventor 赵凌云王丹谢文升
Owner TSINGHUA UNIV
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