Application of ophiopogon polysaccharide extract in preparation of medicines with anti-inflammatory effect

A technology of Ophiopogon japonicus polysaccharides and extracts, applied in the field of medical use of Ophiopogon japonicus polysaccharide extracts

Inactive Publication Date: 2018-09-21
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, there is no bibliographical report that the polysaccharide extract of Radix Ophiopogon japonicus in the range of molecular weight 3000-6500Da has anti-inflammatory application

Method used

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  • Application of ophiopogon polysaccharide extract in preparation of medicines with anti-inflammatory effect
  • Application of ophiopogon polysaccharide extract in preparation of medicines with anti-inflammatory effect
  • Application of ophiopogon polysaccharide extract in preparation of medicines with anti-inflammatory effect

Examples

Experimental program
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Effect test

Embodiment 1

[0018] The preparation of embodiment 1 Ophiopogon japonicus polysaccharide extract (peak molecular weight 5000-6500Da)

[0019] 1) Extraction of Ophiopogon japonicus total polysaccharides:

[0020] Take dried Ophiopogon japonicus tuber, reflux three times with 5-6 times the weight of 70%-95% ethanol, discard it; extract the dregs with water for 2-3 times. Concentrate the water extract to 0.5-1g / ml under reduced pressure, add 75%-95% ethanol until the alcohol content reaches 60%-80%, let it stand overnight, pour off the supernatant, repeat the alcohol precipitation once for precipitation, and freeze-dry , to obtain light yellow Ophiopogon japonicus total polysaccharide powder;

[0021] 2) Purification of Ophiopogon japonicus polysaccharide:

[0022] Take the total polysaccharides of Ophiopogon japonicus, add water to dissolve, and centrifuge to remove the precipitate; add 1-2 times the volume of 70%-95% ethanol to the supernatant to precipitate, let it stand overnight, and re...

Embodiment 2

[0023] The preparation of embodiment 2 Ophiopogon japonicus polysaccharide extract (peak molecular weight 3000-5000Da)

[0024] 1) Extraction of Ophiopogon japonicus total polysaccharides:

[0025] Take dried Ophiopogon japonicus tuber, reflux three times with 5-6 times the weight of 70%-95% ethanol, discard it; extract the dregs with water for 2-3 times. Concentrate the water extract to 0.5-1g / ml under reduced pressure, add 75%-95% ethanol until the alcohol content reaches 60%-80%, let it stand overnight, pour off the supernatant, repeat the alcohol precipitation once for precipitation, and freeze-dry , to obtain light yellow Ophiopogon japonicus total polysaccharide powder;

[0026] 2) Purification of Ophiopogon japonicus polysaccharide:

[0027] Take the total polysaccharides of Ophiopogon japonicus, add water to dissolve, and centrifuge to remove the precipitate; add 1-2 times the volume of 70%-95% ethanol to the supernatant to precipitate, let it stand overnight, and re...

Embodiment 3

[0028] The preparation of the tablet that embodiment 3 contains Ophiopogon japonicus polysaccharide extract

[0029] Prescription (1000 tablets):

[0030]

[0031] Add microcrystalline cellulose, micropowder silica gel, stearic acid, and lactose to the polysaccharide extract of Ophiopogon japonicus according to the prescription amount, mix them evenly, make granules, add magnesium stearate, mix well, and compress into tablets, 1000 tablets (each tablet contains Ophiopogon japonicus polysaccharide extract 0.1g). The recommended oral dosage for adults is 1-4 tablets / time, 2-3 times a day.

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Abstract

The invention discloses application of an ophiopogon polysaccharide extract (having a peak tip molecular weight within a range of 3,000-6,500Da) in preparation of medicines or health foods with an anti-inflammatory effect. A an abdominal obesity mouse model is used for investigating an experiment of inhibiting the secretion of inflammatory factors and the mRNA expression level of tissue inflammatory factor in serum of mice by using the ophiopogon polysaccharide extract, so that the anti-inflammatory effect of the ophiopogon polysaccharide extract is evaluated; the embodiments and animal results thereof prove that the ophiopogon polysaccharide extract has the remarkable anti-inflammatory effect; therefore, the ophiopogon polysaccharide extract can be used for preparing the medicines or health foods which have the anti-inflammatory effect.

Description

technical field [0001] The invention relates to the field of medicine and health care products, in particular to the medical use of an extract of Ophiopogon japonicus polysaccharides. Background technique [0002] Inflammatory diseases or diseases involving inflammatory mechanisms cover multiple system organs of the human body, including the respiratory system, cardiovascular system, nervous system, digestive system, and urinary system. Some chronic inflammations are also likely to induce the formation of malignant tumors. Strengthening the development of drugs to treat inflammatory diseases is of great significance, which is conducive to expanding the selection of clinical treatment options. [0003] Cytokines are an important class of inflammatory mediators, which can be divided into two categories according to their influence and effect on inflammatory response, and inflammatory factors that promote inflammatory response, such as tumor necrosis factor-α (TNF-α) and interl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P29/00A23L33/125A23L33/105
CPCA61K31/715A23L33/105A23L33/125A23V2002/00A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61P29/00A23V2200/30A23V2250/1886A23V2250/21A23V2250/51084A23V2250/612A23V2300/14
Inventor 王源冯怡
Owner SHANGHAI UNIV OF T C M
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