A kind of adventitious bud regeneration method of Fujian mountain cherry

A technology of cherry blossom and mountain cherry, applied in the field of adventitious bud regeneration of Fujian mountain cherry, can solve the problems of difficult adventitious bud regeneration, high induction survival rate, shortening the time of plant regeneration, etc., so as to shorten the plant regeneration cycle, facilitate differentiation and induction The effect of high rate and survival rate

Active Publication Date: 2020-03-24
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The object of the present invention is to provide a method for the regeneration of adventitious buds of Fukien cherry blossoms. The mature embryo cotyledons of Fukien cherry blossoms are used as explants to induce adventitious buds to perform plant regeneration. The induced survival rate is high and the plant regeneration is greatly shortened. It solves the problem of difficult regeneration of adventitious buds of Fujian mountain cherry, and the operation steps are simple and easy, which can be used for the establishment of genetic transformation system, which is helpful for the genetic improvement of Fujian mountain cherry

Method used

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  • A kind of adventitious bud regeneration method of Fujian mountain cherry
  • A kind of adventitious bud regeneration method of Fujian mountain cherry
  • A kind of adventitious bud regeneration method of Fujian mountain cherry

Examples

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Embodiment 1

[0044]Embodiment 1 A kind of method that utilizes the cotyledon of mature embryo of Fujian mountain cherry to carry out adventitious bud regeneration, the cultivation steps are as follows:

[0045] 1) Collection of materials: Around May, the mature fruits of Fujian mountain cherry blossoms are selected.

[0046] 2) Disinfection of explants: After cleaning the ripe fruits of Fujian mountain cherry blossoms with a soft brush dipped in detergent, rinse them under running water for 1 hour, disinfect them with 70% alcohol for 30 seconds on an ultra-clean bench, and then use 20% alcohol to disinfect them. Disinfect with sodium hypochlorite solution for 30 minutes, add 0.5% Tween-20 solution in the solution, rinse with sterile water 3 times at last, cut the seed coat of the fruit after disinfection with a scalpel in the ultra-clean workbench, take out two cotyledons, spare.

[0047] 3) Adventitious bud differentiation induction: Cut the cotyledons of mature embryos into pieces and i...

Embodiment 2

[0061] In the cultivation method of this embodiment, except that the disinfection method in step 2) is different from that of Example 1, the rest of the steps are the same as in Example 1.

[0062] The explant disinfection method of this embodiment is as follows: after cleaning the mature fruits of Fujian mountain cherry blossoms with a soft brush dipped in detergent, rinse them under running water for 2 hours, and disinfect them with 70% alcohol for 60 seconds on a super-clean bench , 0.1% raw mercury solution for disinfection for 15 minutes, finally rinsed with sterile water for 5 times, and set aside, the explant contamination rate of the present embodiment was 0.

Embodiment 3

[0064] In the cultivation method of this embodiment, except that the adventitious bud differentiation induction medium in step 3) is different from that of Example 1, the rest of the steps are the same as that of Example 1.

[0065] The adventitious bud differentiation induction medium in this example uses WPM medium as the basic medium, and contains 2 mg / L thiadizuron and 1 mg / L indolebutyric acid, the pH value is 5.8, and the induction rate is about 65%.

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Abstract

The invention discloses a regenerating method for adventitious buds of Fujian cerasus campanulata. The method comprises the following steps: cotyledons of mature germs of the Fujian cerasus campanulata are used as explants, the adventitious buds are generated through induction, elongation cultivation is performed on the adventitious buds, rooting cultivation is performed to obtain rooting seedlings, seedling exercising is performed, and domestication is performed to obtain seedlings of the Fujian cerasus campanulata. The regenerating method for the Fujian cerasus campanulata disclosed by the invention is established, the induction rate of the adventitious buds is up to 70%, and the survival rate is 95%; the operation steps are simple and easy to implement, the difficult problem of regeneration of the adventitious buds of the Fujian cerasus campanulata is solved, and an induction survival rate is high; regeneration time of plants is greatly shortened, and a regeneration cycle of the Fujian cerasus campanulata is shortened to 60-90 days; and the method disclosed by the invention can be used for establishment of a system of genetic transformation, facilitates genetic improvement of the Fujian cerasus campanulata, has great significance for tissue culture rapid propagation, genetic engineering breeding and research of the Fujian cerasus campanulata, and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of plant regeneration, and in particular relates to a method for regeneration of adventitious buds of Fujian mountain cherry. Background technique [0002] Fujian Mountain Cherry Blossom (Cerasus campanulata) is a deciduous tree of the genus Cerasus in the Rosaceae (Rosaceae). , Oval-elliptic or obovate-elliptic, leaves leathery, length 4-7 cm, width 2-3 cm. [0003] Fujian mountain cherry blossoms in late winter and early spring every year. It blooms first and then leaves. The calyx tube is bell-shaped, and the petals are obovate and oblong. The flowers are drooping, shaped like corymbs. The full flowering period is from late January to early March. After flowering, the fruit is oval or oval drupe, and the fruit matures from April to June. [0004] Fujian mountain cherry has the advantages of early flowering, strong stress resistance and wide adaptability. It is a rare wild species of cherry in my country and is n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 关媛李水根朱建军李记开李秀芬
Owner SHANGHAI ACAD OF AGRI SCI
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