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Method and device for processing repetitive sequences of circulating tumor deoxyribonucleic acid (DNA)

A DNA repetition and sequence technology, applied in the field of genetic engineering, can solve the problem of low accuracy, achieve the effect of improving processing accuracy and reducing manual errors

Active Publication Date: 2018-09-28
GENE CRAB BIOTECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Embodiments of the present invention provide a method and device for processing repetitive sequences of circulating tumor DNA, so as to at least solve the technical problem of low accuracy in the processing method of sequencing data in the prior art to delete or mark repetitive sequences in sample sequencing

Method used

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  • Method and device for processing repetitive sequences of circulating tumor deoxyribonucleic acid (DNA)
  • Method and device for processing repetitive sequences of circulating tumor deoxyribonucleic acid (DNA)
  • Method and device for processing repetitive sequences of circulating tumor deoxyribonucleic acid (DNA)

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Embodiment 1

[0043] According to an embodiment of the present invention, an embodiment of a method for processing circulating tumor DNA repeat sequences is provided. It should be noted that the steps shown in the flowcharts of the accompanying drawings can be implemented in a computer system such as a set of computer-executable instructions and, although a logical order is shown in the flowcharts, in some cases the steps shown or described may be performed in an order different from that shown or described herein.

[0044] figure 1 It is a flowchart of a method for processing circulating tumor DNA repeat sequences according to an embodiment of the present invention, such as figure 1 As shown, the method includes the following steps:

[0045] Step S102, obtaining the sequencing data and the reference genome sequence of the circulating tumor DNA to be detected, wherein the sequencing data is the data obtained by high-throughput sequencing of the circulating tumor DNA to be detected, and the...

Embodiment 2

[0185] According to an embodiment of the present invention, an embodiment of a device for processing circulating tumor DNA repeat sequences is provided.

[0186] Figure 4 It is a schematic diagram of a processing device for circulating tumor DNA repeat sequences according to an embodiment of the present invention, such as Figure 4 As shown, the device includes:

[0187] The obtaining module 42 is used to obtain the sequencing data and the reference genome sequence of the circulating tumor DNA to be detected, wherein the sequencing data is the data obtained by high-throughput sequencing of the circulating tumor DNA to be detected, and the sequencing data includes: multiple pairs of double-end sequences.

[0188] Specifically, the above-mentioned circulating tumor DNA to be detected can be the gene sequence extracted from the patient's blood, lymph fluid, interstitial fluid, cerebrospinal fluid and other body fluids. In the embodiment of the present invention, ctDNA extracted...

Embodiment 3

[0198] According to an embodiment of the present invention, an embodiment of a storage medium is provided. The storage medium includes a stored program, wherein when the program is running, the device where the storage medium is located is controlled to execute the above-mentioned method for processing circulating tumor DNA repeat sequences.

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Abstract

The invention discloses a method and device for processing repetitive sequences of circulating tumor deoxyribonucleic acid (DNA). The method comprises the following steps of: obtaining sequencing dataof to-be-detected circulating tumor DNA and a reference genomic sequence, wherein the sequencing data are obtained by carrying out high-throughput sequencing on the to-be-detected circulating tumor DNA and comprise a plurality of pairs of double-ended sequences; comparing the sequencing data with the reference genomic sequence to obtain a first comparing result, wherein the first comparing resultat least comprises genomic positions, base sequences and corresponding base mass value sequences of the double-ended sequences; and obtaining at least one consensus sequence and a base mass value sequence corresponding to each consensus sequence on the basis of the first comparing result. By means of the method disclosed by the invention, the technical problem that the accuracy is low when repeated sequence deleting or labeling is carried out on sample sequencing by adopting a sequencing data processing method in the prior art is solved.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method and device for processing circulating tumor DNA repeat sequences. Background technique [0002] During the process of division and proliferation, tumor cells will undergo apoptosis, death, and necrosis, and will also actively release DNA fragments carrying tumor mutations into body fluids, that is, circulating tumor DNA (circulating tumor DNA, ctDNA for short), which mostly exists in In body fluids such as blood, synovial fluid and cerebrospinal fluid, especially in plasma free DNA (cell-free DNA, cfDNA). Through the sequencing of ctDNA, the genomic region where the base sequence changes (mutation) occurs on the DNA molecule of the tumor cell is detected, which can effectively reflect the patient's response to the treatment; after the drug response is detected, the tumor may become resistant to the drug treatment, ctDNA detection can also track the generation of drug-...

Claims

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Application Information

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IPC IPC(8): G06F19/22
CPCG16B30/00
Inventor 郭昊于佳宁韩天澄宋雪林小静
Owner GENE CRAB BIOTECH CO
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