Anglerfish skin-sourced collagen peptide with liver damage repairing effect

Abstract

Anglerfish skin-sourced collagen peptide with a liver damage repairing effect uses anglerfish skin as a raw material, and is prepared through pretreatment, enzymolysis with alkaline protease and ultrafiltration, wherein the pretreatment comprises the following specific steps: firstly washing the fish skin, soaking in distilled water with the temperature of 2-8 DEG C for 10-30min, then soaking in a6-12-fold sodium hydroxide solution with the concentration of 0.08-0.12mol / L by volume of for 20-24h, then washing by using clear water till a neutral state, draining the water, swelling for 9-15h byusing glacial acetic acid with the concentration of 0.08-0.12mol / L, and pulverizing to obtain anglerfish skin powder; the enzymolysis comprises the following specific steps: adding distilled water and the alkaline protease into the anglerfish skin powder, performing enzymolysis, deactivating enzyme after completion of the enzymolysis, centrifuging and taking a supernatant; the ultrafiltration comprises the following specific steps: performing ultrafiltration by using ultrafiltration membranes with the molecular weight of 8-10KDa, 5-8KDa, 3-5KDa, 1-3KDa and 1KDa below sequentially, and takinga part under the ultrafiltration membrane with the molecular weight of 1KDa below, and removing salt through nanofiltration, and then performing freeze drying. The anglerfish skin-sourced collagen peptide is applied to treatment of the nonalcoholic fatty liver disease.

Description

technical field [0001] The invention relates to an Ankang cortex collagen peptide capable of repairing liver damage and its use, and belongs to the technical field of bioactive substance extraction. Background technique [0002] nonalcoholic fatty liver disease ( Nonalcoholic fatty liver disease, NAFLD ) is a pathological syndrome mainly characterized by hepatic steatosis and fat accumulation. The current treatment methods mainly adopt conservative treatments such as weight loss therapy, lipid-lowering therapy and angiotensin converting enzyme inhibitors, but there is still no specific effective method for conservative treatment , with poor results. In recent years, Chinese researchers have done a lot of research on the protective effect of biologically active substances extracted from marine animals on liver damage. [0003] A large amount of Ankang skin is discarded during the processing of Ankang products. Fish skin is rich in collagen and is an excellent raw material f...

AI Technical Summary

Problems solved by technology

[0002] nonalcoholic fatty liver disease ( Nonalcoholic fatty liver disease, NAFLD ) is a pathological syndrome mainly characterized by hepatic steatosis and fat accumulation. The current treatment methods mainly adopt conservative treatments such as weight loss therapy, lipid-lowering therapy and angiotensin converting enzyme inhibitors, but there is still no specific effective method for conservative treatment , poorly