A kind of supramolecular hydrogel and preparation method thereof
A technology of supramolecular hydrogel and anhydrous potassium carbonate is applied in the field of biomedical material preparation, which can solve the problems of insufficient mechanical strength of supramolecular hydrogel, and achieve the effects of strong molecular recognition ability, simple method and improved mechanical strength.
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Embodiment 1
[0029] A preparation method of supramolecular hydrogel, comprising the following steps:
[0030] (1) Preparation of 4-arm-PEG-BPY 2+
[0031] a. Dissolve 1 g of four-arm PEG (4-arm-PEG, Mn=20000 g / mol) and 5 g of anhydrous potassium carbonate in 30 mL of dichloromethane at room temperature, and then in an ice-water bath with 100 ~ Magnetic stirring at a speed of 200r / min for 40min;
[0032] b. Add 0.1 g of bromoacetyl bromide to the product obtained in step a, seal it, take out the ice pack, and stir magnetically at a speed of 100 to 200 r / min for 24 hours at room temperature;
[0033] c. Centrifuge the product obtained in step b at 3500r / min. After centrifugation for 10min, collect the supernatant, add excess anhydrous ether for precipitation, then filter, and freeze-dry the filter cake at -80°C to obtain 4-arm-PEG-Br;
[0034] d. Add 1 mol of 4-arm-PEG-Br and 1 mol of 4,4'-bipyridine to 30 mL of anhydrous acetonitrile at the same time, heat and reflux at 80°C for 24 hour...
Embodiment 2
[0042] A preparation method of supramolecular hydrogel, comprising the following steps:
[0043] (1) Preparation of 4-arm-PEG-BPY 2+
[0044] a. Dissolve 1 g of four-arm PEG (4-arm-PEG, Mn=20000 g / mol) and 7 g of anhydrous potassium carbonate in 30 mL of dichloromethane at room temperature, and then in an ice-water bath with 100 ~ Magnetic stirring at a speed of 200r / min for 40min;
[0045]b. Add 0.1 g of bromoacetyl bromide to the product obtained in step a, seal it, take out the ice pack, and stir magnetically at a speed of 100 to 200 r / min for 24 hours at room temperature;
[0046] c. Centrifuge the product obtained in step b at 3500r / min. After centrifugation for 10min, collect the supernatant, add excess anhydrous ether for precipitation, then filter, and freeze-dry the filter cake at -80°C to obtain 4-arm-PEG-Br;
[0047] d. Add 1 mol of 4-arm-PEG-Br and 1.1 mol of 4,4'-bipyridine to 30 mL of anhydrous acetonitrile at the same time, heat and reflux at 80°C for 24 hou...
Embodiment 3
[0055] A preparation method of supramolecular hydrogel, comprising the following steps:
[0056] (1) Preparation of 4-arm-PEG-BPY 2+
[0057] a. Dissolve 1 g of four-arm PEG (4-arm-PEG, Mn=20000 g / mol) and 10 g of anhydrous potassium carbonate in 30 mL of dichloromethane at room temperature, and then in an ice-water bath with 100 ~ Magnetic stirring at a speed of 200r / min for 40min;
[0058] b. Add 0.1 g of bromoacetyl bromide to the product obtained in step a, seal it, take out the ice pack, and stir magnetically at a speed of 100 to 200 r / min for 24 hours at room temperature;
[0059] c. Centrifuge the product obtained in step b at 3500r / min. After centrifugation for 10min, collect the supernatant, add excess anhydrous ether for precipitation, then filter, and freeze-dry the filter cake at -80°C to obtain 4-arm-PEG-Br;
[0060] d. Add 1 mol of 4-arm-PEG-Br and 1.1 mol of 4,4'-bipyridine to 30 mL of anhydrous acetonitrile at the same time, heat and reflux at 80°C for 24 h...
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