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Antioxidant activity of alcohol extract of endophytic fungal mycelium of hosta ventricosa and application of alcohol extract of endophytic fungal mycelium

A technology of endophytic fungi and purple flower hosta, applied in the field of microorganisms, can solve problems such as unreported research

Inactive Publication Date: 2018-10-02
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the "endosymbiosis theory" that the host plant and its endogenous microorganisms live together for a long time to produce the same or similar secondary metabolites synthesis pathway, the applicant isolated and screened endophytic fungi with strong antioxidant activity from Hosta japonica. Research not reported

Method used

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  • Antioxidant activity of alcohol extract of endophytic fungal mycelium of hosta ventricosa and application of alcohol extract of endophytic fungal mycelium
  • Antioxidant activity of alcohol extract of endophytic fungal mycelium of hosta ventricosa and application of alcohol extract of endophytic fungal mycelium
  • Antioxidant activity of alcohol extract of endophytic fungal mycelium of hosta ventricosa and application of alcohol extract of endophytic fungal mycelium

Examples

Experimental program
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Effect test

example 1

[0023] Example 1: Typical isolation of endophytic fungi HoV1, HoV4, and HoV8 from Hosta japonica

[0024] 1. Collection of plant samples: Fresh and healthy rhizomes of Hosta japonica were collected from Chengdu Campus of Sichuan Agricultural University, rinsed under running water for 12 h, rinsed with 6% sodium hypochlorite for 25 s, blotted the surface liquid with sterile filter paper, rinsed with sterile water for 3 times, absorbed Dry surface liquid for surface disinfection. After surface disinfection, check the cleanliness of the ultra-clean workbench, check the rinsing solution, and screen the sterile tissue blocks with the plant tissue imprinting method.

[0025] 2. Isolation and purification of endophytic fungi: Cut the above-mentioned sterile tissue into small pieces with a diameter of about 0.5 cm on a sterile workbench, place them in PDA medium, and culture them upside down in a constant temperature fungal incubator at 25 °C. When the hyphae of endophytic fungi grow...

Embodiment 2

[0034] Example 2: Antioxidant activity test of endophytic fungi HoV1, HoV4, HoV8 mycelia of Hosta violet

[0035] 1. Resuscitation and activation of strains: Inoculate the fungi stored in the 4°C refrigerator into PDA medium, place them in a 25°C fungal constant temperature incubator and cultivate them for 7 days, activate them, pick mycelia around the activated colonies and inoculate them into In PDA medium, cultured in a fungal incubator at 25°C for 7 days to restore the normal growth of the fungus.

[0036]2. Preparation of fermentation products of endophytic fungi HoV1, HoV4, and HoV8 of Hosta violet: Inoculate the strains on PDA plates and culture at 25 °C for 7 days. On the workbench, use a hole puncher to punch a 6 mm diameter bacterial cake along the edge of the colony, and inoculate it into a 250 mL Erlenmeyer flask containing 100 mL of LPDB medium; incubate at 25 °C, 170 r / min for 15 days, until the mycelium After the liquid surface of the culture liquid was complet...

Embodiment 3

[0053] Embodiment 3: preparation test:

[0054] The product contains 5% alcohol extract of HoV1, HoV4, HoV8 mycelium, 2% sodium soap is added as emulsifier, and 1% fragrance is added to prepare antioxidant cream.

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Abstract

The invention relates to endophytic fungi (HoV1, HoV4, and HoV8) of hosta ventricosa and an isolation and culture method of the endophytic fungi and an antioxidant effect of an alcohol extract of theendophytic fungi. The endophytic fungi are isolated from hosta ventricosa, and are relatively high in total antioxidant activity and relatively strong in scavenging capability of DPPH free radical andABTS+ free radical.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the preparation and application of alcohol extracts of three kinds of endophytic fungi of Hosta japonica. Background technique [0002] Hosta ventricosa is a perennial herbaceous plant of the genus Hosta in the family Liliacea. It is mainly distributed in the temperate and subtropical regions of Asia at an altitude of 500-2400 m. It is native to China, Korea and Japan. The whole herb can be used as medicine, and it is a commonly used medicinal material in traditional Mongolian medicine. Among them, the hosta flower is mainly used for boils, snake bites, etc., the hosta leaf is mainly used for sore throat, urination discomfort, burns, etc., and the hosta root eliminates swelling and pain, and removes toxins, etc. . A variety of cardiovascular diseases in humans are related to oxygen free radicals. However, synthetic antioxidants are toxic to a certain extent. I...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12N1/14A61K36/896A61K36/06A61P39/06A61K8/9794A61K8/9728A61Q19/08C12R1/645
CPCA61K36/06A61K36/896A61Q19/08A61K8/9728A61K8/9794A61P39/06C12N1/02C12N1/14
Inventor 梁晓霞吴强陈楠胡云锴贺常亮殷中琼尹立子李丽霞邹元峰宋旭
Owner SICHUAN AGRI UNIV
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