Real-time quantitative PCR method for detecting components of meat product

Abstract

The invention discloses a real-time quantitative PCR method for detecting components of meat product. The real-time quantitative PCR method comprises the following steps: extracting genomic DNA of a meat product sample and a standard sample; designing PCR amplification primers required by the genomic DNA of the meat product sample and the standard sample according to the polymorphism difference ofmitochondrial NADH oxidoreductase 1, correspondingly preparing a PCR reaction system for PCR amplification, and reading the Ct value during the amplification process; judging the reliability of PCR amplification according to the contrast between the value Ct of the NADH oxidoreductase 1 in PCR amplification products and the Ct value of 18S rRNA; and judging whether the meat product sample contains any component to be detected according to the difference delta Ct of the Ct value of the NADH oxidoreductase 1 of the genomic DNA amplification products of the meat product sample and the standard sample. The real-time quantitative PCR method can be used for simultaneously detecting one or more of the components with different species sources in the meat product, and has accurate and reliable detection results.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a real-time quantitative PCR method for detecting components of meat products. Background technique [0002] For the meat industry, adulteration occurs from time to time, mainly by adding some low-priced meat (such as bamboo rat meat, duck, goose, etc.) to replace some high-priced meat (such as beef, mutton, etc.) Finished product sales. The commonly used meat product identification methods include protein identification (including polyacrylamide gel electrophoresis, enzyme-linked immunosorbent assay, liquid chromatography, etc.) and DNA identification (including polymerase chain reaction PCR, RFLP labeling, etc.), among which , DNA identification has the advantage of lower requirements for the type and purity of the material to be identified. However, there is also the problem of inaccurate identification results in the existing DNA identification methods. Contents of...

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Problems solved by technology

However, there is still the problem of inaccurate identification results in the existing DNA identification methods

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