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Method for culturing Cordyceps sinensis by using silkworm larvae and Cordyceps militaris

A technology for cultivating Cordyceps and Cordyceps, which is applied in the field of Cordyceps cultivation, can solve the problems of affecting the breeding rate, prone to inoculation infection, and low yield, and achieve the effects of avoiding inoculation infection, low cost and high yield

Inactive Publication Date: 2018-10-12
王道富
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing Cordyceps cultivation, after the cultivation of live strains is completed, when cultivating Cordyceps, the strains need to be injected into silkworm chrysalis with a syringe, such as Chinese patent CN102440149A, which requires high operation and low yield, and the most important thing is that it is prone to inoculation infection. Phenomenon, which affects the sloppy cultivation in the later stage, and even terminates the cultivation process

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for cultivating Cordyceps with silkworm larvae and pupae, comprising the steps of:

[0021] (1) Separation and breeding of bacterial strains: from the commercially available natural Cordyceps sinensis, select a relatively robust Cordyceps strain, add a certain amount of glucose, acetic acid, potassium dihydrogen phosphate, peptone and magnesium sulfate mixture and soak for 7 days; The mixture of glucose, acetic acid, potassium dihydrogen phosphate, peptone and magnesium sulfate is mixed according to the mass ratio of 2:1:1:1:1, and the amount added is such that the Cordyceps strain can be completely submerged in it.

[0022] (2) Infect the host: select the 5th instar silkworm larvae and pupae with uniform skin color as the host, and at 23°C, in an incubator with 80% humidity, put the processed bacterial classification of step (1) together with the host and irradiated with 300nm wavelength ultraviolet light for 20 days. After seeing the host surface covered with...

Embodiment 2

[0029] A method for cultivating Cordyceps with silkworm larvae and pupae, comprising the steps of:

[0030] (1) Separation and breeding of bacterial strains: from the commercially available natural Cordyceps sinensis, select a relatively robust Cordyceps strain, add a certain amount of glucose, acetic acid, potassium dihydrogen phosphate, peptone and magnesium sulfate mixture and soak for 7 days; The mixture of glucose, acetic acid, potassium dihydrogen phosphate, peptone and magnesium sulfate is mixed according to the mass ratio of 2:1:1:1:1, and the amount added is such that the Cordyceps strain can be completely submerged in it.

[0031] (2) Infect the host: select the 5th instar silkworm larvae and pupae with uniform skin color as the host, at 20 DEG C, in an incubator with a humidity of 70%, put the processed bacterial classification of step (1) together with the host and irradiated with 200nm wavelength ultraviolet light for 15 days. After seeing the host surface covered...

Embodiment 3

[0038] A method for cultivating Cordyceps with silkworm larvae and pupae, comprising the steps of:

[0039] (1) Separation and breeding of bacterial strains: from the commercially available natural Cordyceps sinensis, select a relatively strong Cordyceps strain, add a certain amount of glucose, acetic acid, potassium dihydrogen phosphate, peptone and magnesium sulfate mixture and soak for 7 days; The mixture of glucose, acetic acid, potassium dihydrogen phosphate, peptone and magnesium sulfate is mixed according to the mass ratio of 2:1:1:1:1, and the amount added is such that the Cordyceps strain can be completely submerged in it.

[0040](2) Infect the host: select the 5th instar silkworm larvae and pupae with uniform skin color as the host, at 22 DEG C, in an incubator with a humidity of 75%, put the processed bacterial classification of step (1) together with the host and irradiated with 250nm wavelength ultraviolet light for 18 days. After seeing the host surface covered ...

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Abstract

The invention relates to the field of culturing of Cordyceps sinensis, specifically to a method for culturing the Cordyceps sinensis by using silkworm larvae and Cordyceps militaris. The method comprises the following steps: (1) separation and breeding of fungi; (2) infection of hosts; (3) preparation of a nutrient solution; (4) culturing and fermentation of silkworm cultured Cordyceps sinensis; and (5) soilless culturing and harvesting of Cordyceps sinensis. The method provided by the invention has the following advantages: the whole process lasts for 60 to 80 days; operation is simple and convenient; the fungi are not needed to be injected into silkworm pupas with a syringe; the situation of inoculation infection is avoided from occurrence; the culturing yield of the Cordyceps sinensis can reach 80% or more; and the Cordyceps sinensis cultured by using the silkworm larvae and the Cordyceps militaris has the advantages of high yield, low cost, zero pollution, and greatly-improved cordycepin content.

Description

technical field [0001] The invention relates to the field of cordyceps cultivation, in particular to a method for cultivating cordyceps with silkworm larvae and pupae. Background technique [0002] Cordyceps has been used as Tibetan folk medicine for thousands of years, and the whole plant is used as medicine. Together with ginseng and deer antler, it is known as the three high-level tonics, which have the effects of nourishing the lungs and kidneys, stopping bleeding and resolving phlegm. Cordyceps contains cordycepic acid, also known as D-mannitol, which is the main active ingredient of Cordyceps. According to literature reports, it contains 17 kinds of amino acids such as fatty acid, aspartic acid, and threonine, and various elements such as Al, Si, K, and Na. Element. [0003] Due to the good therapeutic and health effects of Cordyceps sinensis, the demand at home and abroad is increasing day by day. In addition, Cordyceps sinensis is only distributed in specific area...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01K67/033A01K67/04
CPCA01G18/00A01G18/20A01K67/033A01K67/04
Inventor 王道富
Owner 王道富
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