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Biological reaction chip and preparation method thereof

A biological reaction and chip technology, applied in the field of digital PCR biological detection, can solve the problems of complex process steps, high development cost, low sample loading rate, etc., and achieve the effects of simple process steps, improved reliability, and easy storage.

Active Publication Date: 2018-10-16
臻准生物工程山西有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a bioreaction chip and its preparation method, which are used to solve the problems of high development cost, complicated process steps, and low loading rate of the bioreaction chip in the prior art.

Method used

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  • Biological reaction chip and preparation method thereof
  • Biological reaction chip and preparation method thereof
  • Biological reaction chip and preparation method thereof

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preparation example Construction

[0054] Such asfigure 1 As shown, the present invention provides a kind of preparation method of bioreactor chip, comprising:

[0055] Step S1: providing a microcavity chip, the microcavity chip includes a substrate and a plurality of reaction units formed in the substrate.

[0056] Step S2: forming a hydrophilic coating on the platform surface of the substrate and the inner wall of the reaction unit.

[0057] Step S3: forming a hydrophobic coating on the surface of the substrate by means of wet chemical modification or transfer printing.

[0058] Step S4: obtaining a bioreaction chip in which the reaction liquids in each reaction unit do not interfere with each other.

Embodiment 1

[0060] Such as figure 2 As shown, the present embodiment provides a method for preparing a bioreaction chip, and the method for preparing a bioreaction chip includes:

[0061] Step S11 : providing a microcavity chip 3 .

[0062] Specifically, the step of preparing the microcavity chip 3 includes:

[0063] Step S111: if image 3 As shown, a substrate 1 is provided, and the material of the substrate 1 includes but not limited to silicon, silicon oxide, glass, plastic and silica gel, and is not limited to this embodiment.

[0064] Step S112: if Figure 4 As shown, micro-holes are formed on the substrate 1 by micro-processing means such as photolithography and etching, and each micro-hole is used as a reaction unit 2 . In this example, 1cm 2 30,000 (or more than 30,000) micropores are formed on the substrate 1 to obtain high-density reaction units to ensure the reliability of calculation results. In practical applications, the density of micropores can be adjusted according ...

Embodiment 2

[0076] Such as Figure 7 As shown, the present invention provides a method for preparing a bioreaction chip. The difference from Example 1 is that the reaction unit 2 is a through hole, and the hydrophobic coating 5 is formed by wet chemical modification. Specifically include the following steps:

[0077] Step S12: providing a microcavity chip 3.

[0078] Specifically, such as Figure 8 As shown, a substrate 1 is provided, and micropores are formed on the substrate 1 by micro-processing means such as photolithography and etching, and each micropore is used as a reaction unit 2 . In this embodiment, the reaction unit 2 is a through hole. Other structures are similar to those in Embodiment 1, and will not be repeated here.

[0079] Step S22: forming Al on the surface of the microcavity chip 3 by atomic layer deposition 2 o 3 coating.

[0080] Specifically, such as Figure 9 As shown, in this embodiment, Al is formed on the platform surface of the substrate 1 and the inne...

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Abstract

The invention provides a biological reaction chip and a preparation method of the biological reaction chip. The preparation method comprises the following steps: providing a microcavity chip, whereinthe microcavity chip comprises a substrate and a plurality of reaction units formed in the substrate; forming hydrophilic coatings on a platform face of the substrate and the inner side walls of the reaction units; and forming a hydrophobic coating on the surface of the substrate by adopting a wet-process chemical modification method or a transfer printing method, so that the biological reaction chip without interference of reaction liquid in the reaction units is obtained. For the biological reaction chip and the preparation method of the biological reaction chip, through the hydrophobic treatment for the platform face of the substrate and the hydrophilic treatment for the inner walls of the reaction units, the interference among the reaction units is eliminated; meanwhile, the processingsteps are simple, the sample loading rate reaches up to 95% or above, fluorescence signals can be smoothly detected, the reliability of the dPCR detection result can be improved, in addition, the micro-cavity type chip is convenient to store, and reexamination analysis can be carried out at any time.

Description

technical field [0001] The invention relates to the field of digital PCR biological detection, in particular to a biological reaction chip and a preparation method thereof. Background technique [0002] Polymerase chain reaction (Ploymerase Chain Reaction, PCR) is a molecular biology technique used to amplify specific DNA fragments. Since its inception in 1985, PCR technology has gone through three stages: qualitative (endpoint PCR), relative quantitative (real-time quantitative PCR, Real-time PCR, qPCR for short) and absolute quantitative (digital PCR, dPCR for short). [0003] As the second-generation PCR technology, qPCR technology has developed into the most commonly used quantitative analysis technology, and is widely used in clinical disease diagnosis, inspection and quarantine, food safety and other fields. The analysis results of qPCR are highly dependent on the cycle threshold (Cycle threshold, Ct value), and the Ct value is determined by the PCR efficiency. When ...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00
CPCC12Q1/6851C12Q2531/113
Inventor 郭枫
Owner 臻准生物工程山西有限公司
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