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Detection method of succinylcholine chloride in peripheral blood

A technology of succinylcholine chloride and a detection method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficulty in qualitative detection of succinylcholine chloride components, and achieves high accuracy, strong binding effect, and good efficiency. Effect

Active Publication Date: 2018-10-16
ANHUI MEDICAL UNIV
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  • Abstract
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Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a method for detecting succinylcholine chloride in peripheral blood, which overcomes the problem that the detection of succinylcholine chloride in the human body is difficult to be qualitative. Changes, can accurately identify whether the serum contains succinylcholine chloride, this method has high accuracy and high efficiency, and provides a good means of identification for forensic testing

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  • Detection method of succinylcholine chloride in peripheral blood
  • Detection method of succinylcholine chloride in peripheral blood

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Embodiment

[0023] A method for detecting succinylcholine chloride in peripheral blood, the method comprising the following steps:

[0024] (1) Use buffer to configure albumin (HSA) stock solution (3×10 -5 mol / L), kept at constant temperature; dissolve standard succinylcholine chloride in deionized water to prepare 1.0×10 -3 mol / L succinylcholine chloride solution;

[0025] (2) Measure the CD spectrum of the prepared HSA stock solution and the albumin sample of the suspected poisoned person with a circular dichrograph and record them;

[0026] (3) Put HSA stock solution (3×10 -5 mol / L) diluted to 3.0×10 -6 mol / L, using a circular dichrograph to detect and measure the CD spectrum in the 200-300nm wavelength range, record; after the diluted HSA stock solution interacts with succinylcholine chloride, determine the CD in the 200-300nm wavelength range Atlas, record;

[0027] (4) Compare the changes of α-helix content before and after the interaction between HSA and succinylcholine chloride, using the...

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Abstract

The invention provides a detection method of succinylcholine chloride in peripheral blood, which relates to the technical field of medicine detection. The detection method is mainly to detect whetheralbumin of a peripheral blood sample contains succinylcholine chloride or not by virtue of a detection way combining a circular dichroism spectrometry and infrared spectrometry. By adopting the detection method, the problem that the succinylcholine chloride component in the human body is difficult to qualitatively detect can be solved; whether the serum contains the succinylcholine chloride or notcan be accurately determined by virtue of the characteristic variation of a spectrogram obtained by the detection method of the invention; and the detection method is high in accuracy, good in efficiency and capable of providing a good identification means for the detection of medical jurisprudence.

Description

Technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for detecting succinylcholine chloride in peripheral blood. Background technique [0002] Succinylcholine, also known as succinylcholine and scoline, is a commonly used depolarizing muscle relaxant in China. It is a skeletal muscle relaxant in clinical practice and was once listed as a B-grade organic by the country Very toxic. This drug has a fast action and a short duration, and its muscle relaxation effect is 1.8 times that of myostatin. After intravenous injection of this drug, succinylcholine chloride will interact with N in the body 2 The competitive combination of choline receptors blocks the transmission of nerve impulses, resulting in the failure of nerve signals to be transmitted to the muscles, and large areas of paralysis and relaxation of the muscles, which first cause transient muscle tremor, and muscle relaxation occurs within one minute. It usually st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 姜荣陈林陈志武解启文孟刚盛洁万阳
Owner ANHUI MEDICAL UNIV