A method for extracting chlorogenic acid from fresh leaves of Eucommia ulmoides by utilizing biotechnology

A technology of eucommia chlorogenic acid and fresh leaves of eucommia ulmoides, applied in chemical instruments and methods, preparation of organic compounds, separation/purification of carboxylic acid esters, etc., can solve the problems of low honeysuckle resources and high cost of chlorogenic acid, etc. Achieve the effects of improving structural strength and service life, shortening extraction time, and increasing extraction rate

Active Publication Date: 2021-03-02
ANHUI MEIJI FLAVORING FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few honeysuckle resources in the market and the price is high. The cost of extracting chlorogenic acid from honeysuckle is relatively high. Extracting chlorogenic acid from Eucommia can solve the above problems

Method used

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  • A method for extracting chlorogenic acid from fresh leaves of Eucommia ulmoides by utilizing biotechnology
  • A method for extracting chlorogenic acid from fresh leaves of Eucommia ulmoides by utilizing biotechnology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) select 20kg Eucommia ulmoides fresh leaf that plucks in May-August and beat through beater;

[0034] (2) After adding 80kg of pure water at 40°C and pH 6, use 20kHz frequency ultrasonic wave to assist extraction for 30min, and filter to obtain the crude extract of eucommia chlorogenic acid;

[0035] (3) Add 20 g of a stabilizer to the crude extract of Eucommia chlorogenic acid, and stir at 30° C. for 10 min;

[0036] (4) The crude extract of eucommia chlorogenic acid in step 3 was mixed with 5.5m 3 / h flow rate, flow through the purified resin, and then rinse the resin with absolute ethanol 0.5 times the volume of the purified resin bed, leaching out a small amount of chlorogenic acid remaining in the resin, and collecting the filtrate after the resin flows out;

[0037] (5) Concentrate the filtrate in step 4 under reduced pressure at -0.05-0.09MPa and 40°C until the solvent is completely recovered, then vacuum-dry at -0.05-0.09MPa and 60°C to obtain the finished c...

Embodiment 2

[0045] (1) select 20kg Eucommia ulmoides fresh leaf that plucks in May-August and beat through beater;

[0046] (2) After adding 80kg of pure water at 40°C and pH 6, use 20kHz frequency ultrasonic wave to assist extraction for 30min, and filter to obtain the crude extract of eucommia chlorogenic acid;

[0047] (3) Add 20 g of a stabilizer to the crude extract of Eucommia chlorogenic acid, and stir at 30° C. for 10 min;

[0048] (4) The crude extract of eucommia chlorogenic acid in step 3 was mixed with 5.5m 3 flow rate per hour, flow through the purified resin, and then rinse the resin with absolute ethanol 0.5 times the volume of the purified resin bed, leaching out a small amount of chlorogenic acid remaining in the resin, and collecting the filtrate after the resin flows out;

[0049] (5) Concentrate the filtrate in step 4 under reduced pressure at -0.05-0.09MPa and 40°C until the solvent is completely recovered, then vacuum-dry at -0.05-0.09MPa and 60°C to obtain the fini...

Embodiment 3

[0098] Based on Example 1, set the comparative example 1 that does not add chitosan acetic acid solution, the comparative example 2 that does not add modifier, the comparative example 3 that removes impurities by HPD826 resin, do not add epoxy soybean oil and hydrogenated castor The comparative example 4 of sesame oil.

[0099] Using Examples 1-2 and Comparative Examples 1-4 to extract chlorogenic acid in the fresh leaves of Eucommia ulmoides, the purity and extraction rate of chlorogenic acid are shown in Table 1.

[0100] The purity and extraction efficiency of table 1 chlorogenic acid

[0101]

[0102]

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Abstract

The invention discloses a method for extracting chlorogenic acid from fresh leaves of Eucommia ulmoides by using biotechnology, and relates to the technical field of plant extraction. The present invention uses epoxy resin and chitosan as raw materials to make a purified resin through cross-linking polymerization. A layer of chitosan adsorption film is formed inside the resin. Through the double-layer adsorption of resin and adsorption film, chlorogenic acid can be The impurities in the resin are completely absorbed in the resin without destroying the structure and physiological activity of chlorogenic acid; the stabilizer synthesized from polyglutamic acid and flavanol can enhance the resistance of chlorogenic acid to external high temperature and high oxygen, Protect chlorogenic acid from inactivation; the invention adopts ultrasonic-assisted extraction, which greatly shortens the extraction time, improves the extraction rate of chlorogenic acid, is simple and efficient in operation, and is suitable for industrial production.

Description

Technical field: [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting chlorogenic acid from fresh leaves of Eucommia ulmoides by using biotechnology. Background technique: [0002] Eucommia is a plant of the genus Eucommia in the Eucommia family, a deciduous tree. my country has a very wide area suitable for the growth of Eucommia, and the land that can be used to plant Eucommia is more than 10 million hectares. At present, the cultivation area of ​​Eucommia in China is about 350,000 hectares, accounting for more than 99% of the total planting area of ​​Eucommia in the world. It has unique resource advantages. It can produce 4 million tons of leaves. [0003] Chlorogenic acid belongs to phenylpropanoid compounds, which is a kind of organic acid containing carboxyl group and o-diphenol group, and its chemical name is 3-caffeoylquinic acid. Chlorogenic acid widely exists in higher dicotyledonous plants and ferns, ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C67/48C07C67/56C07C69/732B01J20/26C08G81/00
CPCB01J20/24B01J20/262C07C67/48C07C67/56C08G81/00C07C2601/14C07C69/732
Inventor 王启标
Owner ANHUI MEIJI FLAVORING FOOD
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