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Use of interferon alpha-1a in the preparation of medicines for treating cancer

A technology of interferon α and its use, which is applied in the field of medicine and biology, and can solve problems such as lack of formula

Active Publication Date: 2020-11-13
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the second-line medication mainly depends on the specific situation of the patient. Generally, gemcitabine alone or in combination with other chemotherapy drugs is considered, but there is still a lack of standardized second-line medication formulations

Method used

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  • Use of interferon alpha-1a in the preparation of medicines for treating cancer
  • Use of interferon alpha-1a in the preparation of medicines for treating cancer
  • Use of interferon alpha-1a in the preparation of medicines for treating cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Example 1: Effect of IFN-α-1a on Human Cervical Cancer

[0096] Materials and methods

[0097] 1. Cell Lines, Cell Culture and Reagents

[0098] The human cervical cancer cell line HeLa was obtained from the Institute of Basic Medical Sciences. HeLa cells were incubated at 37°C, 5% CO 2 were grown in DMEM high glucose medium (Gibco) supplemented with 10% FBS.

[0099] Rabbit anti-caspase 3 antibody, rabbit anti-Bim antibody, rabbit anti-Bcl-xL antibody, rabbit anti-cytochrome c antibody, mouse anti-β-actin antibody, and rabbit anti-GAPDH antibody were purchased from Santa Cruz Biotechnology. Rabbit anti-PARP1 and rabbit anti-cleaved PARP1 antibodies were purchased from Epitomics Inc. Mouse anti-caspase 8 antibody and rabbit anti-caspase 10 antibody were purchased from Bioworld Technology Inc. Rabbit anti-caspase 4, rabbit anti-cleaved-caspase-8 antibody, rabbit anti-cleaved-caspase-3 antibody were purchased from Proteintech Group Inc. Peroxidase-conjugated second...

Embodiment 2

[0136] Example 2: The effect of IFN-α-1a on laryngeal cancer

[0137] Materials and Methods

[0138] Human laryngeal carcinoma cell line Hep2 cells were obtained from the Institute of Basic Medical Sciences. Hep2 cells were incubated at 37°C, 5% CO 2 were grown in DMEM high glucose medium (Gibco) supplemented with 10% FBS.

[0139] The rest are the same as the materials and methods in Example 1.

[0140] result

[0141] 1. Verification of IFN-α-1a expression after transfection of pcDNA3.0-IFN-α-1a plasmid into Hep2 cells

[0142] Different doses of constructed pcDNA3.0-IFN-α-1a plasmids were transiently transfected into Hep2 cells, and after 48 hours of culture, Hep2 cells were collected and RNA was extracted. RT-RCR obtained the HA-tagged IFN-α-1a gene fragment, which was verified by agarose gel electrophoresis, and the experimental group obtained IFN-α-1a-HA with the correct molecular size (see Figure 6A). The experiment proved that the pcDNA3.0-IFN-α-1a plasmid was su...

Embodiment 3

[0155] Example 3: The effect of IFN-α-1a on pancreatic cancer

[0156] Materials and Methods

[0157] Pancreatic cancer MIA PaCa-2 cells were from the Institute of Basic Medical Sciences. MIA PaCa-2 cells were incubated at 37°C, 5% CO 2 were grown in DMEM high glucose medium (Gibco) supplemented with 10% FBS.

[0158] The rest are the same as the materials and methods in Example 1.

[0159] result

[0160] 1. IFN-α-1a has a significant pro-apoptotic effect on poorly differentiated pancreatic cancer cell line MIA PaCa-2 (Int. J. Cancer: 19, 128-135 (1977)). Analysis of flow cytometry results found that the experimental group added IFN-α-1a (such as Figure 12A (shown) co-cultured MIA PaCa-2 cells, the number of apoptotic cells was significantly more than that of MIA PaCa-2 cells without IFN-α-1a treatment in the control group, and with the increase of IFN-α-1a dose, The increase of early apoptosis and late apoptosis was more. However, the experimental group was transfect...

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Abstract

The application relates to application of interferon alpha-1a in preparing drugs for treating cancer, specifically to the application of the interferon alpha-1a in preparing drugs for treating cervical cancer, laryngeal carcinoma and pancreatic cancer. The interferon alpha-1a can obviously inhibit the proliferation of HeLa, HeG2, SW1990 and MIA-PaCa-2 cells and promote apoptosis of the cells. Theresult suggests that the interferon alpha-1a possibly has the characteristics and potential of resisting the cervical cancer, the laryngeal carcinoma and the pancreatic cancer and provides a new clueand theoretical basis for clinical treatment of cancer.

Description

technical field [0001] This application relates to the field of medical biology. More specifically, it relates to the use of interferon alpha-1a in the preparation of medicines for treating cervical cancer, laryngeal cancer or pancreatic cancer. Background technique [0002] Interferon (IFN) is a cytokine with a wide range of biological activities. It has been studied for the treatment of cancer because of its anti-virus, immune system regulation, and inhibition of tumor cell proliferation. According to the different types of cells produced by interferon, the receptors that interact with interferon, and the biological activity of interferon, interferon is mainly divided into three categories: interferon I, interferon II and interferon III, which have a structure similarities and functional differences. Interferons used clinically, such as interferon α and interferon β, mainly belong to class I interferons. Due to racial differences in disease types and genetic profiles, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/21A61P35/00
CPCA61K38/212
Inventor 刘力辛晓磊
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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