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Gene detection device

A technology for gene detection and extraction container, applied in the field of medical devices, can solve the problems of cumbersome steps, inconvenient to carry instruments, unable to observe the detection results, etc., and achieve the effects of simple operation, high reliability and convenient use

Pending Publication Date: 2018-11-02
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the disadvantages of the prior art that it is inconvenient to carry instruments, and the samples need to be extracted, amplified, and detected step by step, the steps are cumbersome and the detection results cannot be observed, and a method for quickly extracting target nucleic acids, Portable device with integrated amplification and detection

Method used

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  • Gene detection device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0045] This embodiment takes the adulteration detection of mutton as an example for illustration:

[0046] 1 Experimental materials

[0047] Cylinder; 1.5ml centrifuge tube; rubber stopper; syringe; lysate (137mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 2mM KH2PO4, 10mM Tris-HCl and 1mM EDTA); RPA reagent; SYBR Green I or EVA Green fluorescent dye; Small UV flashlight; metal bath or water bath

[0048] Such as figure 1 As shown, the portable device includes a long cylinder 3 and a centrifuge tube 5 , and the long cylinder 3 and the centrifuge tube 5 are sealed and connected by a rubber stopper 4 . The top of the cylinder is sealed with another rubber stopper 2, which can be removed, put the sample in the cylinder, seal it with the rubber stopper, and then use the syringe 1 to draw 200 μl of the prepared lysate, and inject it into the cylinder through the needle The sample was lysed internally, left at room temperature for 1 min, and 2 μL of the supernatant was sucked and inserted int...

example 2

[0049] Example 2: Sample Extraction System

[0050] Put the sample in the cylinder, cover it with a rubber stopper, and use a syringe to draw 200 μL of lysate into the cylinder. This lysate can destroy the cell wall or cell membrane and expose the gene sample to the solution. It can be used after standing for one minute. The supernatant was used as the template for amplification, and was continuously injected into a 1.5ml centrifuge tube containing the reaction solution.

example 3

[0051] Example 3: Nucleic Acid Amplification

[0052] The characteristic of nucleic acid constant temperature amplification technology is that the whole process of amplification reaction is carried out at a single temperature without special amplification equipment, unlike PCR reaction, which needs to experience dozens of cycles of temperature changes. This characteristic of the constant temperature amplification technology makes it support the isothermal amplification platform used in the portable device of the present invention, and the temperature required for the amplification is realized by the heating ring. For example, RPA reaction, fluorescent dye, reaction buffer, etc. are added to the reaction centrifuge tube in advance, and the DNA template extracted from the cylinder is amplified at 37-42°C for 20 minutes in the test tube through a syringe. As time goes by, the fluorescent dye produces a higher fluorescent signal due to the combination with the double-stranded DNA,...

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Abstract

The invention relates to the medical equipment field, in particular to a gene detection device integrating extraction, amplification and visual detection. The device comprises a syringe, a DNA extraction container and a centrifugal tube. The top opening of the DNA extraction container is covered with a first sealing plug, and the bottom end of the DNA extraction container is connected to the centrifugal tube through a second sealing plug. The method provided by the invention is suitable for determining all target nucleic acid sequences, and can complete extraction amplification and result detection under an airtight condition, at the end of detection, the sealed device can be all discarded in a safe place without opening, thus greatly lowering the possibility of contamination, and whetherspecifically amplified target nucleic acid exists can be quickly and reliably determined. At the same time, the DNA extraction step can be completed without centrifuge and other large-scale instruments. The whole device is low in cost and very convenient to use, and does not need heavy auxiliary equipment.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to a gene detection device integrating extraction, amplification and visual detection. Background technique [0002] In recent years, as people pay more and more attention to health and the environment, pathogen detection, prenatal diagnosis, and food safety testing that are closely related to life and health have become more and more important. At present, nucleic acid is extracted by traditional nucleic acid extraction methods, and molecular detection by PCR amplification method is very sensitive, specific and informative. Unfortunately, currently available nucleic acid extraction and nucleic acid detection methods are not suitable or of limited utility for use at the sampling site because they require delicate, bulky and expensive instruments, specialized laboratory materials, or rely on Multiple operations using intervention. Most samples for molecular testing are shipped to cen...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00C12Q1/6876
CPCC12N15/1003C12Q1/6876
Inventor 陈爱亮李婷婷张桂兰王之莹
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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