A kind of marine fungus derived from Difidocus gorgonian and its application in the preparation of antibacterial drugs

A technology of marine fungi and compounds, which is applied to the preparation of antibacterial drugs, fungi, and sugar derivatives, and can solve problems such as rare structures

Active Publication Date: 2022-01-21
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Diphenyl ether glycoside compounds are still structurally rare compounds so far, only 8 such compounds have been reported, and only one of them is derived from sponge fungi in the ocean, and no joints derived from corals and coral fungi have been seen. Phenyl ether glycosides have been reported

Method used

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  • A kind of marine fungus derived from Difidocus gorgonian and its application in the preparation of antibacterial drugs
  • A kind of marine fungus derived from Difidocus gorgonian and its application in the preparation of antibacterial drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Cultivation of the marine fungus Phoma sp.

[0016] The medium used for the culture of the fungus Phoma sp. contains 1.0% glucose (percentage by weight, the same below), 0.1% yeast extract, 0.2% peptone, 1.0% agar, 3.0% coarse sea salt, and the rest is water. Tube slant, fungal strains were grown at 28°C for 5 days.

[0017] (2) Fermentation of marine fungus Phoma sp.

[0018] The fermentation medium used in the fermentation culture of the fungus Phoma sp. is that each 500mL Erlenmeyer flask contains 80g of rice, 2g of coarse sea salt, and 120mL of water. Use 50 500mL Erlenmeyer flasks for fermentation.

[0019] (3) Separation and extraction of formula (I) and formula (II) compounds of the present invention

[0020] Take the fermented product obtained in step (2), extract it with ethyl acetate for 3 times, concentrate the extract under reduced pressure to obtain a crude extract, first perform normal phase silica gel column chromatography separation, stationary ph...

Embodiment 2

[0024] (1) Cultivation of the marine fungus Phoma sp.

[0025] The culture medium contains glucose 0.10%-10% (weight percentage, the same below), yeast extract 0.01%-4.0%, peptone 0.01%-4.0%, agar 0.10%-6.0%, coarse sea salt 0.05%-10%, and the rest It is water; the cultivation temperature is 5-45°C; the cultivation time is 3-10 days.

[0026] (2) Fermentation of marine fungus Phoma sp.

[0027] The fermentation medium contains 50-150g of rice, 0.05-10g of coarse sea salt, and 50-150mL of water in each 500mL Erlenmeyer flask; the fermentation temperature is 5-45°C; the fermented product is obtained after 20-50 days of fermentation.

[0028] (3) Separation and extraction of formula (I) and formula (II) compounds of the present invention

[0029] Get the fermented product of step (2) gained, extract 2-6 times with ethyl acetate, obtain ethyl acetate extract, extract is concentrated under reduced pressure to obtain crude extract, first carry out normal phase silica gel column ch...

Embodiment 3

[0032] The marine fungus Phoma sp. is cultured in the strain medium, and then the above-mentioned marine fungus is fermented and cultivated in the fermentation medium, and the fermentation product is extracted with ethyl acetate for 2 to 6 times, and the extract is decompressed The crude extract was obtained by distillation, and after chromatographic separation, two colorless amorphous powder compounds were obtained, which were compounds of formulas (I) and (II), and the structural confirmation data were consistent with the corresponding data in Example 1. Wherein the strain culture medium contains glucose, yeast extract, peptone, agar, coarse sea salt, water, and the fermentation medium contains rice, coarse sea salt, water; the chromatographic separation is followed by normal phase silica gel column chromatography Separation, gel column chromatography and high performance liquid chromatography.

[0033] In order to explore a wider range of methods suitable for preparing the ...

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PUM

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Abstract

The invention belongs to marine medicines, and in particular relates to a marine fungus derived from Cordica gorgonianus and its application in the preparation of antibacterial medicines. The strain preservation information of the marine fungus Phoma sp. of the present invention: the name of the preservation unit: China Microbial Strain Preservation Management Committee General Microbiology Center; the preservation unit address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Chinese Academy of Sciences Microbiology Institute; date of deposit: April 1, 2017; deposit number: CGMCC No.13881; classification name: Phoma sp.

Description

technical field [0001] The invention belongs to marine medicines, and in particular relates to a marine fungus derived from Cordica gorgonianus and its application in the preparation of antibacterial medicines. Background technique [0002] The ocean, due to its special environment such as high pressure, high salinity, low oxygen, and low light, enables it to produce some compounds with novel structures. Diphenyl ether glycoside compounds are still structurally rare compounds so far, only 8 such compounds have been reported, and only one of them is derived from sponge fungi in the ocean, and no joints derived from corals and coral fungi have been seen. Phenyl ether glycosides were reported. Contents of the invention [0003] The present invention provides a marine fungus Phoma sp., the fungus is isolated from Gorgoniana difidis, wherein the inventor collected Gorgoniana difidis from Xisha Islands in the South China Sea in September 2008. The strain preservation informati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/44C07H15/203C07H1/00A61K31/7034A61P31/04C12R1/645
CPCC12P19/44C07H1/00C07H15/203C12R2001/645C12N1/145
Inventor 王长云邵长伦史婷齐君
Owner OCEAN UNIV OF CHINA
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