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Anti-C5 full-human derived single-chain antibody C5B3 and application thereof

A single-chain antibody, fully human technology, applied in the field of bioengineering, can solve the problems of complex humanization process, life-threatening, allergic reactions, etc., and achieve the effect of inhibiting the formation of membrane attack complexes and reducing damage

Active Publication Date: 2018-11-06
西安天泓生物科技有限公司
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Problems solved by technology

[0005] At present, monoclonal antibodies that block the C5 complement effect are mainly obtained from mouse sources through humanization. The humanization process is also relatively complicated, and there is still a HAMA reaction after humanization. This reaction affects the therapeutic effect of the antibody and may be harmful to The patient causes secondary injuries, such as allergic reactions, kidney damage, etc., which may be life-threatening in severe cases

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  • Anti-C5 full-human derived single-chain antibody C5B3 and application thereof
  • Anti-C5 full-human derived single-chain antibody C5B3 and application thereof
  • Anti-C5 full-human derived single-chain antibody C5B3 and application thereof

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Embodiment Construction

[0031] Specific embodiments of the present disclosure will be described in detail below in conjunction with the accompanying drawings. It should be understood that the specific embodiments described here are only used to illustrate and explain the present disclosure, and are not intended to limit the present disclosure.

[0032] The present invention is based on a fully human scFv single-chain antibody library, and obtains a fully human anti-C5 single-chain antibody by screening a phage display method, avoiding the HAMA reaction, and has carried out functional verification; it is different from the previous anti-C5 single-chain antibody Compared with the CDR region of the chain antibody, the difference is quite large, and it is a brand new monoclonal antibody drug. The invention is a single-chain antibody with a size of about 30KD and a small protein molecular weight, which is easier to enter the lesion and take effect, especially for brain diseases, which can pass through the...

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Abstract

The invention discloses an anti-C5 full-human derived single-chain antibody C5B3 and application thereof. The anti-C5 full-human derived single-chain antibody C5B3 has a DNA (Deoxyribonucleic Acid) sequence as shown in SEQ ID NO:1. By adopting the antibody, formation of a membrane attacking composition in an inflammation reaction process can be remarkably inhibited, so that damage caused in the inflammation reaction process can be reduced. The invention further discloses application of the anti-C5 full-human derived single-chain antibody C5B3 in preparing medicines for treating autoimmune diseases such as ophthalmoneuromyelitis, multiple sclerosis, systemic lupus erythematosus and myasthenia gravis and / or inflammation and diseases with related symptoms caused by paroxysmal nocturnal hemoglobinuria (PNH), atypical haemolytic uraemic syndrome (AHU) and trauma.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to an anti-C5 fully human single-chain antibody C5B3 and its application. Background technique [0002] C5 is a very important component of complement. The complement system includes more than 30 components, which are widely present on the surface of serum, tissue fluid and cell membrane. Complement is not only an important part of the body's innate immune defense system, but also one of the important mechanisms for antibodies to exert immune effects. And participate in the adaptive immune response and its regulation in different links. During the inflammatory reaction, C5 convertase decomposes C5 into C5a and C5b. C5a is free in the liquid phase and is an important inflammatory mediator. C5b forms a membrane attack complex (MAC) through a series of complement reactions, leading to cell lysis and death. [0003] Complement activation is a highly ordered cascade that exerts a wide range ...

Claims

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Application Information

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IPC IPC(8): C07K16/18A61K39/395A61P37/02A61P29/00A61P7/06A61P13/12A61P7/00A61P7/02
CPCA61K2039/505A61P7/00A61P7/02A61P7/06A61P13/12A61P29/00A61P37/02C07K16/18C07K2317/21C07K2317/56C07K2317/622
Inventor 闫亚平朱文莉
Owner 西安天泓生物科技有限公司
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