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A solution system for promoting recovery of cryopreserved tissues and organs and cell activity

A technology for tissue, organ and cell activity, applied in artificial cell constructs, tissue culture, animal cells, etc., can solve the problems that have not been reported on the cryopreservation solution system of tissues, organs and cells, achieve convenient use and increase the success rate Effect

Active Publication Date: 2022-02-15
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There is no report on the tissue organ and cell cryopreservation solution system that meets all the above requirements

Method used

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  • A solution system for promoting recovery of cryopreserved tissues and organs and cell activity
  • A solution system for promoting recovery of cryopreserved tissues and organs and cell activity
  • A solution system for promoting recovery of cryopreserved tissues and organs and cell activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation of Solution System for Promoting Recovery of Frozen Tissues and Organs and Cell Activity

[0021] According to the component ratios in Table 1, prepare a solution system that promotes the recovery of frozen tissues and organs and cell activity. Each experimental group is configured according to the components and ratios within the protection scope of the claims of this application, and each control group is a certain Components are missing or the mass percentage of components is beyond the protection scope of the claims of this application.

[0022] Table 1 The composition and ultrasonic irradiation treatment parameters of the solution system to promote the recovery of cryopreserved tissues and organs and cell activity

[0023]

[0024]

[0025] Note: " / " means that the item does not exist, and "*" means that the index is beyond the protection scope of the claims of this application.

Embodiment 2

[0026] Activity evaluation of embodiment 2 rat liver

[0027] In this example, the solution system prepared in Example 1 for promoting recovery of cryopreserved tissues and organs and cell activity was used to evaluate the effect of rat liver preservation.

[0028] Several SD rats were isolated to obtain rat livers, which were respectively placed in the solutions of different experimental groups and control groups in Table 1, and cryopreserved under different conditions of -80°C, -40°C, -20°C, and -5°C for 6 After taking it out, it was immediately treated with ultrasonic irradiation for resuscitation (see Table 1 for operating parameters). The activity of the rat liver was evaluated by trypan blue staining, and the results are shown in Table 2.

[0029] Table 2 Evaluation results of rat liver

[0030]

[0031]

[0032] Note: more than 30% activity is considered to have better liver protection ability in rats.

[0033] From the results in Table 2, it can be seen that th...

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Abstract

A solution system for promoting recovery of cryopreserved tissues and organs and cell activity of the present invention uses one or more of water and polyols as the base solution, and polyphenols, citric acid, and poloxamer are added to the base solution and vitamin E, put tissues, organs and cells in this solution system for cryopreservation, and apply the wavelength within the range of 20KHz-1MHz and the sound intensity is not greater than 3W / cm after cryopreservation 2 Cells are irradiated by ultrasonic waves. By adjusting the ratio of different components in the solution, and controlling the wavelength, sound intensity and irradiation time of ultrasonic waves, the biological activity of frozen tissues, organs and cells after recovery can be improved.

Description

technical field [0001] The invention relates to a method for recovering tissue, organ and cell activity, in particular to an in vitro preservation solution of tissue and organ cells in a low temperature environment and a method for using the same. Background technique [0002] At present, the most important way to maintain the activity of animal organs, tissues and cells is to use extremely low temperature for long-term cryopreservation, and then undergo thawing and resuscitation treatment to obtain living cells, but this cryopreservation method tends to reduce cell activity, such as The survival rate of islets and liver cells is only 10-30%. [0003] With the development of surgery and immunosuppressants in recent years, the cases of organ transplantation are increasing day by day. For the treatment of organ transplantation and cell transplantation, the most ideal way is to directly transplant the removed organs or isolated and purified cells from the donor to the recipien...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12N13/00A01N1/02
CPCA01N1/0221A01N1/0226C12N5/0018C12N13/00C12N2500/34C12N2500/30
Inventor 赵应征鲁翠涛陈翩翩徐荷林金冰慧林蒙婷童梦琪江雪郑雅文
Owner WENZHOU MEDICAL UNIV
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