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Passiflora foetida tissue culture method

A tissue culture and dragon fruit technology, applied in the field of plant tissue culture, can solve the problems of no induced somatic embryos and adventitious buds, and achieve the effects of increasing the possibility of material utilization, large quantity and low pollution rate.

Inactive Publication Date: 2018-11-13
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are studies related to tissue culture of dragon fruit in foreign countries, the explants used are mostly found in the seeds, stems and other parts of dragon fruit. There are no related reports on the use of dragon fruit leaves as explants to induce somatic embryos and adventitious buds.

Method used

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  • Passiflora foetida tissue culture method
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  • Passiflora foetida tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: Tissue culture method for inducing somatic embryos (1)

[0049] (1) Preparation of explants

[0050] The test material Longzhu fruit is a seedling with a height of about 12 cm after three months of growth, which is cultivated by seed sowing. Select young, robust, and basically consistent growth dragon fruit leaves as explants, rinse them under tap water, and then wash them with sterile water for 3 times. Put the cleaned leaves on the ultra-clean workbench, first wipe the front and back of the leaves with a cotton ball dipped in a 75% ethanol aqueous solution, and then wash them with sterile water for 3 times, and then use the final concentration as the mass Fraction 0.02% Tween-20 mass fraction 0.1% HgCl 2 The solution was soaked for 3 minutes, and finally washed 5 times with sterile water. The sterilized leaves were cut into small cubes of about 1 cm×1 cm as explants.

[0051] (2) Induction of somatic embryos

[0052] Insert the explants into the som...

Embodiment 2

[0059] Embodiment 2: Tissue culture method for inducing somatic embryos (2)

[0060] (1) Preparation of explants

[0061] The test material Longzhu fruit is a seedling with a height of about 8 cm after three months of growth, which is cultivated by seed sowing. Select young, robust, and basically consistent dragon fruit leaves as explants, rinse them under tap water, and then wash them twice with sterile water. Put the cleaned leaves on the ultra-clean workbench, first wipe the front and back of the leaves with a cotton ball dipped in 75% ethanol water solution by volume fraction, then wash them twice with sterile water, and then use the final concentration as the mass Fraction 0.02% Tween-20 mass fraction 0.1% HgCl 2 The solution was soaked for 5 minutes, and finally washed 4 times with sterile water. The sterilized leaves were cut into small cubes of about 1 cm×1 cm as explants.

[0062] (2) Induction of somatic embryos

[0063] Put the explants into the somatic embryo ...

Embodiment 3

[0070] Embodiment 3: Induce the tissue culture method of somatic embryo and adventitious bud (1)

[0071] (1) Preparation of explants

[0072] The test material Longzhu fruit is a seedling with a height of about 12 cm after three months of growth, which is cultivated by seed sowing. Select young, robust, and basically consistent growth dragon fruit leaves as explants, rinse them under tap water, and then wash them with sterile water for 3 times. Put the cleaned leaves on the ultra-clean workbench, first wipe the front and back of the leaves with a cotton ball dipped in a 75% ethanol aqueous solution, and then wash them with sterile water for 3 times, and then use the final concentration as the mass Fraction 0.02% Tween-20 mass fraction 0.1% HgCl 2 The solution was soaked for 3 minutes, and finally washed 5 times with sterile water. The sterilized leaves were cut into small cubes of about 1 cm×1 cm as explants.

[0073] (2) Induction of somatic embryos and adventitious buds...

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Abstract

The invention discloses a passiflora foetida tissue culture method. The method comprises the following steps: with leaves of passiflora foetida as explants, disinfecting to obtain explants with a lowpollution rate and less damage, inducing the successfully disinfected explants in induction media of different formulations to obtain somatic embryos, adventitious buds and other different products, transferring the somatic embryos into somatic embryo germination media for continuously culturing to obtain germinated somatic embryos, and carrying out plant regeneration culture, transplantation andthe like to obtain passiflora foetida plants growing well. The method has the advantages of convenient material selection, high material utilization rate, large quantity and simple operation, is suitable for factory production, establishes a tissue-culture rapid propagation system with the passiflora foetida leaves as the explants, and provides a seedling technology for rapid propagation of passiflora foetida.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a dragon fruit tissue culture method. Background technique [0002] Dragon fruit (Passiflorafoetida L.), also known as Xianghuaguo, Tianxian fruit, wild fairy peach, etc., is a herbaceous vine of the Passiflora family Passiflora. It is native to the West Indies, and is mainly distributed in Guangdong, Hainan, Fujian, and Taiwan in my country, where it grows on barren hills, grassy slopes, shrubs, or coastal beaches (Wang Dong et al., 2014). [0003] The dragon fruit plant is several meters high, and the whole plant, especially the damaged leaves, often emits a pungent taste; the stem is striped and has flat pilose; the leaves are membranous, pilose on the surface, broadly ovate or oblong-ovate, with a center at the base Shaped with 3-5 lobes, irregular margins, and pinnate veins; petiole with flat pilose; stipules semi-ambulatory, deeply lobed; cymes, flowers whit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 段俊刘艳艳何春梅
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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