Cell mating connection method and cell mating connection device

Abstract

The invention discloses a cell mating connection method and a cell mating connection device. The cell mating connection device comprises a cell mating device; the cell mating device comprises an electron microscope, and an optical tweezers transmitter and a cell chamber which are arranged on the electron microscope; the cell chamber comprises a cell mating chamber and cell storage chambers respectively arranged at the two sides of the cell mating chamber; a spacer plate is arranged between each cell storage chamber and the cell mating chamber; a file used for breaking cell walls is arranged oneach spacer plate; the files are positioned in the cell storage chambers. The method and the device can be used for distinguishing cells in different states, and can be widely applied in the technical field of medicine, thus having a great production practice significance; the method and the device realize precise manipulation of the cells and large-scale cell capture, is high in precision and strong in adjustability, and realizes the operations such as accurate capture, handling, screening and mating of cells.

Description

technical field [0001] The invention relates to the field of cell technology, in particular to a cell mating connection method and device. Background technique [0002] Cells are the simplest unit of life, and the answers to all life problems can be found in cells. In the life science system, one of the reasons why cell biology was born and developed slowly is that the cell itself is a complex life system, and more importantly, so far there is no very satisfactory technical means to use it. Study such basic units of life. The development of modern optics, advanced laser technology and image display technology provides high-tech means for cell biology research. In the mid-1980s, Arthur Ashkin of Bell Laboratories in the United States discovered that a focused Gaussian laser beam could capture a single cell or protozoa, which was later developed into a widely used tool for manipulating cells and organelles—laser tweezers. [0003] At present, laser optical tweezers mostly u...

AI Technical Summary

Problems solved by technology

There are many factors causing this "bottleneck": first, the various biological effects caused by irradiance are not only related to the pulse width, energy and wavelength of the laser, but also affected by the nature of the target itself and its physical environment; second, At present, the laser used for laser micromanipulation has a wide pulse width (nanosecond level), a single wavelength and poor stability, while the volume of a single cell is only on the order of a femtoliter (10-15 liters), Therefore, it is difficult to control the working parameters; thirdly, in the past, the research hotspots of laser cell surgery were mostly focused on finding new objects of action

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