Laser optical tweezer microscope

Abstract

The invention relates to a laser optical tweezer microscope. The microscope comprises: a lens cone, an image sensor, a microscopic objective lens, an objective table which can move up and down, a carrying substrate, a support and a digital optical phase conjugate apparatus which is used to generate laser optical tweezer and focus the optical tweezer on an object. The lens cone is fixed on the support. The objective table which can move up and down is connected with the support through a rack. The carrying substrate is placed on the objective table which can move up and down. The microscopic objective lens, which is used to carry out amplification imaging to the object placed on the carrying substrate, is installed on a bottom of the lens cone. The image sensor, which is used to record and amplify the microscopic image, is arranged in the lens cone and is located above the microscopic objective lens. By using the microscope of the invention, focusing accuracy and positioning accuracy of the optical tweezer can be increased. A precision machinery motor is not needed to increase a moving speed of the optical tweezer. An optical tweezer number can be increased. Any one optical tweezer can be independently operated. Through cooperation of a plurality of the optical tweezers, a high-precision complex microscopic operation can be realized.

Description

technical field [0001] The invention belongs to the field of micro-optics and nanotechnology, and more specifically relates to a laser optical tweezers microscope, which is suitable for manipulation, transportation, testing, assembly, processing, and microscopic three-dimensional measurement of cells, DNA, nanoparticles, and nanostructures. Background technique [0002] In the fields of life science, chemistry, nanotechnology, etc., it is necessary to perform various manipulations and tests on tiny objects, such as cell nuclei, DNA chains, molecular groups, and micro-mechanical structures, such as handling, cutting, peeling, grafting, injection, assembly, and curing. , activation, fluorescence detection, electrophoresis detection, etc. Common microscopic manipulation methods include mechanical probes, molecular tweezers, optical tweezers, etc., in which mechanical probes can puncture cells; molecular tweezers can adsorb cells on the front of capillaries to quantitatively meas...

AI Technical Summary

Problems solved by technology

However, the use of holographic dry plates generally can only produce static optical tweezers. Although the number of optical tweezers can be increased a lot, it is very difficult to dynamically move optical tweezers, especially to move each optical tweezers independently according to any path, because the holographic dry plate The interference fringes on the fixed

Although the use of spatial light modulators can dynamically generate and move optical tweezers through optical wavefront control, the accuracy is still limited to a certain extent, because the size of each pixel of the spatial light modulator is generally much larger than the optical wavelength.

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