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Method for building small intestine insulin expression reduction rat model by adenovirus vector

A rat model, adenovirus technology, applied in the direction of viruses/phages, using vectors to introduce foreign genetic material, viruses, etc., can solve problems such as ineffective treatment, achieve low cost, wide application prospects, and good repeatability

Inactive Publication Date: 2018-11-13
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional treatment methods such as diet control, insulin injection and oral hypoglycemic drugs cannot effectively treat

Method used

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  • Method for building small intestine insulin expression reduction rat model by adenovirus vector
  • Method for building small intestine insulin expression reduction rat model by adenovirus vector
  • Method for building small intestine insulin expression reduction rat model by adenovirus vector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Design of adenoviral vectors for down-expression of insulin in the small intestine of rats. Two antisense mRNA vectors containing rat insulin were designed;

[0030] Ins1 Primer:

[0031] Forward: cgGGATCCatggccctgttggtgcact SEQ ID NO:1

[0032] Reverse: cCGGAATTCTTAGTTGCAGTAGTTTCCAGctg SEQ ID NO:2

[0033] BamHI GGATCC

[0034] EcoRI GAATTC

[0035] Ins2 Primer:

[0036] Forward: CGGGATCCatggccctgtggatgcg SEQ ID NO: 3

[0037] Reverse: CCGGAATTCctagttgcagtagttctccagc gaattccgg SEQ ID NO:4

[0038] BamHI GGATCC

[0039] EcoRI GAATTC

[0040] 2. Detection of reduced expression level of adenoviral vector with reduced expression in cells

[0041] After transfection of adenovirus vector in 293A cells, it can be found that 1 week after transfection, cells appear plaques and release adenovirus, such as figure 1 shown.

[0042] 3. Establishment of T2DM rat model

[0043] Using the random number table method, among 55 SPF male SD rats, 40 were randomly selected as...

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Abstract

The invention discloses a method for building a small intestine insulin expression reduction rat model by an adenovirus vector. The method mainly comprises the steps of adenovirus vector building andrat small intestine transfection by the adenovirus vector. The characteristic that the adenovirus vector carries rat antisense mRNA insulin gene is utilized; through rat gavage, the rat small intestine insulin expression reduction can be built within three months; the reduction of rat insulin tolerance and oral glucose tolerance can be caused. The animal model has wide application prospects for further developing medicine for treating diabetes.

Description

technical field [0001] The invention belongs to a method for establishing an animal model, relates to the treatment and prevention of chronic diabetes, and more precisely relates to a method for using an adenovirus vector to construct a small intestine down-expression insulin rat model. Background technique [0002] As a chronic progressive disease, diabetes poses a huge threat to human health. In 2015, the International Diabetes Federation announced that there are about 415 million adult patients with diabetes worldwide, and about 318 million people with impaired glucose tolerance. Among them, China has the largest number of diabetic patients , has reached 110 million. At present, the whole world is facing the unprecedented problem of diabetes epidemic. If no corresponding active measures are taken, it is estimated that by 2040, there will be more than 642 million people with diabetes. However, traditional treatment methods such as diet control, insulin injection and oral ...

Claims

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Application Information

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IPC IPC(8): C12N15/861C12N15/113C12N15/85A01K67/027
CPCA01K67/0275A01K2217/058A01K2227/105A01K2267/0362C12N15/113C12N15/85C12N15/86C12N2710/10043
Inventor 郭刚张瑞孙蓓陈皓
Owner TIANJIN MEDICAL UNIV
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