Method for building small intestine insulin expression reduction rat model by adenovirus vector

Abstract

The invention discloses a method for building a small intestine insulin expression reduction rat model by an adenovirus vector. The method mainly comprises the steps of adenovirus vector building andrat small intestine transfection by the adenovirus vector. The characteristic that the adenovirus vector carries rat antisense mRNA insulin gene is utilized; through rat gavage, the rat small intestine insulin expression reduction can be built within three months; the reduction of rat insulin tolerance and oral glucose tolerance can be caused. The animal model has wide application prospects for further developing medicine for treating diabetes.

Description

technical field [0001] The invention belongs to a method for establishing an animal model, relates to the treatment and prevention of chronic diabetes, and more precisely relates to a method for using an adenovirus vector to construct a small intestine down-expression insulin rat model. Background technique [0002] As a chronic progressive disease, diabetes poses a huge threat to human health. In 2015, the International Diabetes Federation announced that there are about 415 million adult patients with diabetes worldwide, and about 318 million people with impaired glucose tolerance. Among them, China has the largest number of diabetic patients , has reached 110 million. At present, the whole world is facing the unprecedented problem of diabetes epidemic. If no corresponding active measures are taken, it is estimated that by 2040, there will be more than 642 million people with diabetes. However, traditional treatment methods such as diet control, insulin injection and oral ...

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Problems solved by technology

However, traditional treatment methods such as diet control, insulin injection and oral hypoglycemic drugs cannot effectively treat

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