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Method for building animal modeling for chorioretinopathy in diabetes mellitus

A technology for diabetic retinopathy and choroidal lesions, applied in teaching models, educational tools, instruments, etc., can solve the problems of little research on the mechanism of diabetic choroidal neovascularization and its influencing factors, and achieve low cost, convenient and simple production, and good repeatability Effect

Inactive Publication Date: 2006-07-19
周希瑗
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, little research has been done on the mechanism and influencing factors of diabetic choroidal neovascularization.

Method used

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  • Method for building animal modeling for chorioretinopathy in diabetes mellitus
  • Method for building animal modeling for chorioretinopathy in diabetes mellitus
  • Method for building animal modeling for chorioretinopathy in diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Streptozotocin (Streptoztocin, STZ) was dissolved in 0.05mol / L citric acid buffer (pH4.5), configured into a STZ solution with a concentration of 1.25wt%, and injected into the closed group male wistar at an injection amount of 50mg / kg body weight. Rats were intraperitoneally injected with STZ solution at one time, and the qualitative urine sugar and blood sugar were detected 25 hours after the injection. The blood sugar concentration reached 16.65mmol / L and the urine sugar reached 3+--4+, which established a diabetic rat model; then fed the model The retinal choroidal lesion model of diabetic rats was obtained within 60 days.

[0017] The preparation method of the electron microscope specimen prepared from the eyeball of this animal model: Immediately take the retina of the sacrificed rat and put it in 3% glutaraldehyde fixative solution, fix, dehydrate, soak and embed with epoxy resin EPoN812, prepare ultra-thin sections, and routinely use dioxyacetic acid Oil and lea...

Embodiment 2

[0023] Dissolve streptozotocin (Streptoztocin, STZ) in 0.05mol / L citric acid buffer solution (pH4.5), configure the STZ solution with a concentration of 1.25wt%, inject 40mg / kg body weight to the closed group male wistar Rats were intraperitoneally injected with STZ solution at one time, and the qualitative urine sugar and blood sugar were detected 36 hours after the injection. The blood sugar concentration reached 16.85mmol / L and the urine sugar reached 3+--4+, which established a diabetic rat model; then fed the model The retinal choroidal lesion model of diabetic rats was obtained within 60 days.

[0024] The method for preparing paraffin sections from the eyeballs of this animal model: Immediately take the eyeballs of the sacrificed rats, fix them in Verhoeff's solution, and routinely embed them in paraffin after 48 hours. Serial 6 μm sections were used for HE staining and immunohistochemical staining.

[0025] Such as Figure 5 As shown, the model rat retina choroid imm...

Embodiment 3

[0028] Streptozotocin (Streptoztocin, STZ) was dissolved in 0.05mol / L citric acid buffer (pH4.5), configured into a STZ solution with a concentration of 1.25wt%, and injected into the closed group male wistar at an injection amount of 60mg / kg body weight. Rats were injected intraperitoneally with STZ solution at one time, and the qualitative urine sugar and blood sugar were detected 48 hours after the injection. The blood sugar concentration reached 17.05mmol / L and the urine sugar reached 3+--4+, that is, the diabetic rat model was established; then the model was fed The retinal choroidal lesion model of diabetic rats was obtained within 60 days.

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Abstract

The invention discloses a method for establishing diabetes omentum choroids lesion animal model. It first prepares for the streptozocin solution and then injects the solution into the big mouse abdominal cavity to establish the diabetes mouse model; at last it feeds the model for 60 days.

Description

technical field [0001] The invention relates to a method for establishing an animal model, in particular, a method for establishing an animal model for diabetic retinopathy. technical background [0002] At present, Diabetic retinopathy (DR) is one of the most serious lesions in the microvascular complications of diabetes, which can lead to irreversible blindness in both eyes, and is also one of the four major blindness diseases. With the development of indocyanine green choroid angiography (ICGA) and fundus fluorescein angiography (FFA), it was found that diabetic retinopathy occurred at the same time as the choroid also had corresponding changes, thus suggesting that diabetic retinopathy may exist at the same time. In the early 1980s, Saracco JB and others proposed the concept of diabetic choroidopathy (Diabetic choroidopathy, DC). But so far, there are few studies on the mechanism and influencing factors of diabetic choroidal neovascularization. Contents of the inventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G09B23/36
Inventor 周希瑗李琳乔刚
Owner 周希瑗
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