Compound enzyme preparation for reducing TSNAs (tobacco-specific nitrosamines) as well as preparation method and application of compound enzyme preparation
A compound enzyme preparation and nitrosamine technology, which is applied in the field of tobacco leaf processing, can solve the problems of high preparation cost of microbial inoculants, restricting large-scale popularization and application, and large influence of environmental conditions, so as to reduce the content of TSNAs and facilitate large-scale popularization. Apply and improve the effect of quality and safety
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[0026] Example 1
[0027] The composite enzyme preparation for reducing tobacco specific nitrosamines in this embodiment is composed of the following components in mass percentage: neutral protease 0.03%, pectinase 0.02%, xylanase 0.01%, glucoamylase 0.02%, β- Glucanase 0.01%, sterile distilled water 99.91%.
[0028] The preparation method of the above-mentioned composite enzyme preparation involved in this embodiment includes the following steps:
[0029] (1) Prepare raw materials according to the above components;
[0030] (2) Stir and mix the components of step (1) and sterile distilled water according to the stated mass percentage, at a stirring speed of 75 rpm, a mixing temperature of 40°C, and a pH of 5.5, until a composite enzyme preparation suspension is uniformly obtained, namely Compound enzyme preparation.
[0031] The application of the above-mentioned composite enzyme preparation in the preparation of sun-cured tobacco in this embodiment includes the following steps:
[00...
Example Embodiment
[0038] Example 2
[0039] The composite enzyme preparation for reducing the specific nitrosamines of tobacco leaves of this embodiment is composed of the following components in mass percentage: neutral protease 0.02%, pectinase 0.01%, xylanase 0.005%, glucoamylase 0.01%, β- Glucanase 0.005%, α-amylase 0.01%, medium temperature amylase 0.02%, cellulase 0.02%, sterile distilled water 99.90%.
[0040] The preparation method of the above-mentioned composite enzyme preparation involved in this embodiment includes the following steps:
[0041] (1) Prepare raw materials according to the above components;
[0042] (2) Stir and mix the components of step (1) and sterile distilled water according to the stated mass percentage, at a stirring speed of 50 rpm, a mixing temperature of 35°C, and a pH of 5.2, until a composite enzyme preparation suspension is uniformly obtained, namely Compound enzyme preparation.
[0043] The application of the above-mentioned composite enzyme preparation in the pr...
Example Embodiment
[0048] Example 3
[0049] The composite enzyme preparation for reducing the peculiar nitrosamines of tobacco leaves of this embodiment is composed of the following components by mass percentage:
[0050] Tobacco complex enzyme 0.5%, neutral protease 0.01%, pectinase 0.005%, xylanase 0.0025%, glucoamylase 0.005%, β-glucanase 0.0025%, α-amylase 0.005%, medium temperature amylase 0.005 %, cellulase 0.005%, neutral lipase 0.01% and sterile distilled water 99.45%.
[0051] The preparation method of the above-mentioned composite enzyme preparation involved in this embodiment includes the following steps:
[0052] (1) Prepare raw materials according to the above components;
[0053] (2) Stir and mix the components of step (1) and sterile distilled water in accordance with the stated mass percentage, at a stirring speed of 100 rpm, a mixing temperature of 45°C, and a pH of 5.8, until a composite enzyme preparation suspension is obtained uniformly, namely Compound enzyme preparation.
[0054] T...
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