Preparation method of pseudosciaena crocea egg isolated protein-beta-carotene emulsion

A technology of carotene and protein isolation, which is applied in food ingredients, food shaping, food science, etc., can solve the problems of color degradation, biological activity reduction, bioavailability reduction, etc., and achieves a simple and easy preparation method with good stability Effect

Inactive Publication Date: 2018-11-20
DALIAN POLYTECHNIC UNIVERSITY
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Problems solved by technology

[0006] β-carotene usually accelerates its degradation under the action of light, heat, enzymes, metal ions, metal-binding proteins and microorganisms, causing a ser

Method used

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  • Preparation method of pseudosciaena crocea egg isolated protein-beta-carotene emulsion
  • Preparation method of pseudosciaena crocea egg isolated protein-beta-carotene emulsion
  • Preparation method of pseudosciaena crocea egg isolated protein-beta-carotene emulsion

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preparation example Construction

[0036] The present invention provides a preparation method of large yellow croaker roe protein isolate-β-carotene emulsion, taking large yellow croaker roe protein isolate and β-carotene, and the preparation steps are as follows:

[0037] S1. Use a sodium chloride solution with a concentration of 0.1-1mol / L to extract protein from large yellow croaker roe, the ratio of solid to liquid is (1:10)-(1:20) g / mL, centrifuge at 5000-10000rpm / min for 10-30min, Take the supernatant (i.e. protein extract), freeze-dry in vacuum at -25-50°C for 60-72 hours to obtain protein freeze-dried powder for later use;

[0038] S2. Ultrasonic heating of β-carotene at 10-30 kHz, 50-55° C. for 10-20 minutes, dissolving it in corn oil, and preparing an oil phase with a mass concentration of β-carotene of 0.05%-0.1% for use;

[0039] S3. Reconstitute the freeze-dried protein powder obtained in step S1 with PBS of pH 7-9, 5-15 mmol / L, and prepare an aqueous phase with a protein concentration of 2-5 mg / mL...

Embodiment 1

[0043]A preparation method of large yellow croaker roe protein isolate-β-carotene emulsion, taking large yellow croaker roe protein isolate and β-carotene, and its preparation steps are as follows:

[0044] S1, the large yellow croaker roe is carried out protein extraction with the sodium chloride solution of concentration 0.6mol / L, the solid-liquid ratio 1:20g / mL of large yellow croaker roe and sodium chloride solution, 5000rpm / min centrifugal 30min, get supernatant ( That is, protein extract), vacuum freeze-drying at -25°C for 60 hours to obtain protein freeze-dried powder for future use;

[0045] S2. Ultrasonic heating of β-carotene at 10 kHz and 50° C. for 20 minutes to dissolve it in corn oil, and prepare an oil phase with a mass concentration of β-carotene of 0.1% for use;

[0046] S3. Reconstitute the freeze-dried protein powder obtained in step S1 with pH 8, 10 mmol / L PBS, and prepare an aqueous phase with a protein concentration of 2 mg / mL for use;

[0047] S4. Mix t...

Embodiment 2

[0050] S1. Use a sodium chloride solution with a concentration of 0.6mol / L to extract protein from large yellow croaker roe, the ratio of solid to liquid is 1:20g / mL, centrifuge at 5000rpm / min for 30min, take the supernatant (i.e. protein extract), and store at -50°C Vacuum freeze-drying under conditions for 72 hours to obtain protein freeze-dried powder for future use;

[0051] S2. Ultrasonic heating of β-carotene at 10 kHz and 50° C. for 20 minutes to dissolve it in corn oil, and prepare an oil phase with a mass concentration of β-carotene of 0.1% for use;

[0052] S3. Reconstitute the freeze-dried protein powder obtained in step S1 with pH 8, 10 mmol / L PBS, and prepare an aqueous phase with a protein concentration of 2 mg / mL for use;

[0053] S4. Mix the oil phase prepared in step S2 with the water phase prepared in step S3 in a ratio of 3:100 by weight, and disperse at a high speed of 10000r / min for 2min;

[0054] S5, 12000 psi high pressure homogenization 5 times to obta...

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Abstract

The invention discloses a preparation method of a stable pseudosciaena crocea egg isolated protein-beta-carotene emulsion. The preparation method comprises the following steps of S1, preparing preotein freeze-dried powder: performing salt-dissolving protein extraction on pseudosciaena crocea eggs with a sodium chloride solution, performing centrifuging so as to obtain a supernatant, and performingfreeze-drying for standby application; S2, preparing an oil phase: performing ultrasonic heating on beta-carotene so that the beta-carotene dissolves in corn oil, and performing compounding so as toobtain the oil phase for standby application; S3, preparing an aqueous phase: preparing a protein solution of certain concentration with a buffer solution, and compounding the aqueous phase for standby application; S4, performing mixing: mixing the oil phase with the aqueous phase in a certain proportion, and performing high-speed dispersion; and S5, performing homogenizing: and performing high-pressure homogenizing so as to obtain the stable pseudosciaena crocea egg isolated protein-beta-carotene emulsion. According to the preparation method disclosed by the invention, pseudosciaena crocea egg isolated protein is used as an emulsifying agent for embedding beta-carotene, and the obtained pseudosciaena crocea egg isolated protein-beta-carotene product namely the emulsion is high in nutrientvalue and high in stability.

Description

technical field [0001] The invention relates to the technical field of emulsion preparation, in particular to a method for preparing large yellow croaker egg isolate protein-β-carotene emulsion. Background technique [0002] Fat-soluble nutrients refer to a class of substances that can be dissolved in fat and then ingested, digested, and absorbed by the human body. Common fat-soluble nutrients include vitamins, carotenoids, flavonoids, polyphenols, polyunsaturated fatty acids, etc. In order to improve the bioavailability of fat-soluble nutrients in the human body, a common method is to embed them in an oil-in-water emulsion system. [0003] When fat-soluble nutrients are delivered as core materials, there are many ways to protect them from degradation, and protein is often used as an emulsifier to stabilize them. Proteins play a stabilizing role at the oil-water interface by reducing interfacial tension and increasing steric hindrance. At present, plant protein is mostly ...

Claims

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Application Information

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IPC IPC(8): A23L2/38A23L2/52A23L2/70A23L33/105A23L33/17A23P10/35
CPCA23L2/38A23L2/52A23L2/70A23L33/105A23L33/17A23P10/35A23V2002/00A23V2250/211
Inventor 唐越姜卉王笑涵吴海涛林松毅宋亮张安珏韩佳润
Owner DALIAN POLYTECHNIC UNIVERSITY
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