Adipose tissue preserving fluid in vitro and preparation method thereof

A technology of in vitro preservation and adipose tissue, applied in the field of cell engineering, can solve the problem of insufficient contact of preservation solution, and achieve the effect of preventing damage

Active Publication Date: 2018-11-23
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides an in vitro preservation solution for adipose tissue, which can effectively solve the problem that the adipose tissue is suspended on the preservation solution and cannot be fully contacted with the preservation solution when the adipose tissue is preserved in vitro

Method used

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  • Adipose tissue preserving fluid in vitro and preparation method thereof
  • Adipose tissue preserving fluid in vitro and preparation method thereof
  • Adipose tissue preserving fluid in vitro and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] An adipose tissue in vitro preservation solution, which contains 1 mL of autologous platelet lysate, 2 g of sodium hyaluronate, 3 mL of human albumin, 1 g of Vc, 0.2 g of trehalose, 12 mL of temperature-sensitive hydrogel, and 0.5 g of penicillin per 100 mL. , Streptomycin 0.3g, Amphotericin B 0.02g, and the rest of the solute medium is a phosphate buffer with a pH of 7.4.

[0039] Among them, the preparation method of the temperature-sensitive hydrogel is: mixing polyacrylamide, methacrylic acid, acrylic acid and chitosan, then adding ammonium persulfate, stirring uniformly, after standing for 15 hours for reaction, washing the reaction product three times to obtain a temperature-sensitive hydrogel Glue; wherein the weight ratio of polyacrylamide, methacrylic acid, acrylic acid, chitosan and ammonium persulfate is 8:1:0.6:0.15:1.

[0040] The preparation method of the preservation solution is:

[0041] Under aseptic conditions, the above-mentioned components are evenly mixed...

Embodiment 2

[0045] An adipose tissue in vitro preservation solution, which contains 0.5 mL of autologous platelet lysate, 1 mL of human albumin, 0.5 g of Vc, 0.3 g of trehalose, 20 mL of temperature-sensitive hydrogel, 0.6 g of penicillin, and streptomycin per 100 mL. 0.5g, 0.05g amphotericin B, and the rest of the solute medium is a phosphate buffer with a pH of 7.6.

[0046] Among them, the preparation method of the temperature-sensitive hydrogel is: mixing polyacrylamide, methacrylic acid, acrylic acid and chitosan, then adding sodium persulfate, stirring uniformly, after standing for 15 hours, and washing the reaction product 3 times to obtain a temperature-sensitive hydrogel Glue; wherein the weight ratio of polyacrylamide, methacrylic acid, acrylic acid, chitosan and sodium persulfate is 10:0.5:0.8:0.2:0.6.

[0047] The preparation method of the preservation solution is:

[0048] Under aseptic conditions, the above-mentioned components are evenly mixed to prepare a fat tissue in vitro pre...

Embodiment 3

[0052] An adipose tissue in vitro preservation solution, which contains 1.5 mL of autologous platelet lysate, 5 g of sodium hyaluronate, 5 mL of human albumin, 0.1 g of trehalose, 10 mL of temperature-sensitive hydrogel, 0.2 g of penicillin, and chain 0.1g amphotericin, 0.01g amphotericin B, and the rest of the solute medium is a phosphate buffer with a pH of 7.2.

[0053] Among them, the preparation method of the temperature-sensitive hydrogel is: mixing polyacrylamide, methacrylic acid, acrylic acid and chitosan, then adding lauroyl peroxide, stirring evenly, after standing for 15 hours, washing the reaction product 3 times to obtain temperature-sensitive water Gel; wherein the weight ratio of polyacrylamide, methacrylic acid, acrylic acid, chitosan and lauroyl peroxide is 6:0.8:1:0.3:1.

[0054] The preparation method of the preservation solution is:

[0055] Under aseptic conditions, the above-mentioned components are evenly mixed to prepare a fat tissue in vitro preservation so...

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Abstract

The invention discloses an adipose tissue preserving fluid in vitro and preparation method thereof. The adipose tissue preserving fluid in vitro is prepared by following components as per following parts by weight: 0.5-1.5 parts of autologous platelet lysate, 0-5 parts of sodium hyaluronate, 1-5 parts of human albumin, 0-1 part of Vitamin C, 0.1-0.3 parts of trehalose, 10-20 parts of thermo-sensitive hydrogel, 0.3-1.5 parts of antibacterial agents and 80-90 parts of phosphate buffer; the preparation method comprises mixing of above components as corresponding parts by weight under aseptic conditions to prepare the adipose tissue preserving fluid in vitro; the adipose tissue preserving fluid in vitro prepared by the method allows the adipose tissue to be evenly dispersed into the preservingfluid and allows the adipose tissue to fully contact with the preserving fluid, which is beneficial to long-term storage of the adipose tissue.

Description

Technical field [0001] The invention belongs to the technical field of cell engineering, and specifically relates to an adipose tissue in vitro preservation solution and a preparation method thereof. Background technique [0002] Stem cells are a type of self-renewing multipotent cells. With the deepening of research on stem cells, more and more stem cells from different sources have become research hotspots in various research institutions, such as bone marrow origin. Mesenchymal stem cells, placental-derived mesenchymal stem cells, dental pulp-derived mesenchymal stem cells, adipose-derived mesenchymal stem cells, etc. [0003] With the development of adipose tissue engineering, adipose-derived stem cells (in turn, adipose-derived vascular stromal cells) have received more and more attention, and they are mainly used in medical beauty and other fields. Since Zuk et al. discovered fibroblast stem cells with multiple differentiation potentials in adipose tissue in 2001, the multi-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 高雪华孙雯海泉
Owner CHENGDU QINGKE BIOTECH
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