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Method for regulating exosome release, and medical application of method

A technology of exosomes and rapamycin, which is applied in the medical application of regulating the release of exosomes, in the field of regulating the release of exosomes, and can solve problems such as limiting the application of exosomes

Active Publication Date: 2018-11-23
SOUTHERN MEDICAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Methods to regulate exosome formation and secretion are not yet fully understood, limiting exosome-related applications

Method used

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  • Method for regulating exosome release, and medical application of method
  • Method for regulating exosome release, and medical application of method
  • Method for regulating exosome release, and medical application of method

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Embodiment Construction

[0028] The present invention will be further described in conjunction with specific examples, but the content in the examples does not constitute a limitation to the present invention.

[0029] Materials and Methods

[0030] cell culture.

[0031] TSC2+ / + and TSC2- / - mouse embryonic fibroblasts (MEFs), Hela and HEK293 cells were cultured in DMEM medium (Corning) containing 10% FBS (Gibco). pCMV6-GFP-CD63 was purchased from Obio Technologies (Shanghai), and infected Hela cells until they stably express GFP-CD63. The primary antibodies used in the study were anti-CD63 (#ab193349), TSG101 (#ab83) and ALIX (#ab117600) from Abcam, anti-P-S6 (#4858), LC3II (#3868) and mTOR from Cell Signaling ( #2972), anti-S6 (sc-74459) from Santa Cruz Biotech, anti-Rab27A (#17817-1-AP) and Rab27B (#13412-1-AP) from ProteinTech. DAPI for nuclear staining was from Fisher Scientific (#D1306).

[0032] animal.

[0033] Animal experiments were approved by the Animal Research Ethics Committee of So...

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Abstract

The invention provides a method for regulating exosome release, and medical application of the method in exosome release regulation. The exosome release is promoted by inhibiting the activity of rapamycin target protein mTORC1. The activation of the mTORC1 is inhibited by means of rapamycin, so that the exosome release is increased. By depriving nutrients, growth factors and dietary restrictions,the activity of the mTORC1 is inhibited, and the exosome release is promoted. The mTORC1 regulates the exosome release by means of a Rab27A-dependent mechanism. mTOR is associated with Rab27A, and theinteraction between the Rab27A and the mTOR is enhanced by means of rapamycin treatment. The exosome release, accompanied by autophagy, is affected by changes in nutrients and growth factor conditions. According to the exosome release, the cellular contents are released out of cells by means of fusion with the plasma membrane, so that the loss of the cellular contents is caused. The method can regulate the exosome release and provides a feasible scheme for medical application in the exosome release regulation.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for regulating the release of exosomes and its medical application for regulating the release of exosomes. Background technique [0002] Exosomes are tiny membrane-bound vesicles (40–200 nm in diameter) that are released extracellularly by many different types of cells under physiological and pathological conditions. Although exosomes were originally considered as cellular waste products, these extracellular vesicles have received increasing attention as mediators of intercellular communication. Exosomes contain signaling proteins, miRNA, mRNA, DNA and lipids, which can regulate various life activities of recipient cells during transport. Exosomes can be found in many types of bodily fluids, including plasma and urine. The secretion level of exosomes is closely related to many physiological and pathological activities, often indicating health, stress and disease conditions. Rec...

Claims

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Application Information

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IPC IPC(8): C12N5/00
CPCC12N5/00C12N2501/405
Inventor 白晓春崔忠凯邹文翀赖明强张月
Owner SOUTHERN MEDICAL UNIVERSITY
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