Reagent for fast identifying Wen county iron stick yam and identification method thereof
A technology of iron rod yam and reagents, which is applied in the field of crop detection, can solve the problems of inaccurate discrimination and inaccurate discrimination of medicine farmers, and achieve the effect of simple operation
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Embodiment 1
[0034] The invention discloses a reagent for quickly identifying Wenxian Tiegon yam, the reagent is a glucose identification reagent, and the identification reagent and glucose undergo a color reaction.
[0035] The identification reagent is an alkaline buffer solution containing a water-soluble colorless tetrazolium salt.
[0036] The water-soluble colorless tetrazolium salt is 2,3,5-triphenyl tetrazolium chloride (red tetrazolium), 2,2'-diphenyl 5,5'-(3,4-dimethyl Oxyphenyl)-3,3'-dimethoxy-4,4'-bistetrazolium salt (tetrazolium blue), 2,5-diphenyl-3-(1-naphthyl)-2H -tetrazole chloride (tetrazole violet), 3,3'-(4,4'-biphenyl)bis(2,5-diphenyl-2H-tetrazole chloride) (neotetrazole chloride) One of them, or other structures. Different water-soluble colorless tetrazolium salts show different colors when identifying glucose.
[0037] Since glucose is a reducing sugar, the water-soluble colorless tetrazolium salt will be reduced to an insoluble colored formazan when it encounters ...
Embodiment 2
[0047] The invention discloses a reagent for quickly identifying Wenxian Tiegon yam, the reagent is a glucose identification reagent, and the identification reagent and glucose undergo a color reaction.
[0048] The identification reagent is an alkaline buffer solution containing a water-soluble colorless tetrazolium salt.
[0049] The water-soluble colorless tetrazolium salt is 2,3,5-triphenyl tetrazolium chloride (red tetrazolium).
[0050] The pH of the alkaline buffer solution is 10.
[0051] The pH of the alkaline buffer solution is adjusted by an aqueous sodium carbonate solution.
[0052]The preparation method of the alkaline buffer solution containing the water-soluble colorless tetrazolium salt is as follows: first prepare an aqueous solution of red tetrazolium with a mass concentration of 0.5%, add an alkaline buffer solution 3 times the volume of the aqueous solution, and then add the aqueous solution 10 times the volume of water, shake well. Wherein, the red tet...
Embodiment 3
[0056] The invention discloses a reagent for quickly identifying Wenxian Tiegon yam, the reagent is a glucose identification reagent, and the identification reagent and glucose undergo a color reaction.
[0057] The identification reagent is an alkaline buffer solution containing a water-soluble colorless tetrazolium salt.
[0058] The water-soluble colorless tetrazolium salt is 2,2'-diphenyl 5,5'-(3,4-dimethoxyphenyl)-3,3'-dimethoxy-4,4' - bistetrazolium salt (tetrazolium blue).
[0059] The pH of the alkaline buffer solution is 12.
[0060] The pH of the alkaline buffer solution is adjusted by an aqueous calcium hydroxide solution.
[0061] The preparation method of the alkaline buffer solution containing water-soluble colorless tetrazolium salt is as follows: first prepare an aqueous solution of tetrazolium blue with a mass concentration of 1%, add an alkaline buffer solution with 5 times the volume of this aqueous solution, and then add this aqueous solution for 5 Doubl...
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