Reagent for fast identifying Wen county iron stick yam and identification method thereof

A technology of iron rod yam and reagents, which is applied in the field of crop detection, can solve the problems of inaccurate discrimination and inaccurate discrimination of medicine farmers, and achieve the effect of simple operation

Active Publication Date: 2018-11-23
HIGH & NEW TECH RES CENT OF HENAN ACAD OF SCI +1
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned detection methods are highly subjective. Some counterfeit iron stick yams, such as iron stick varieties grown in other places, and some similar to iron sticks such as Xi Shi, chicken skin rough, and small white mouth, etc., even experienced drug farmers cannot accurately identify them. At present, there is no method for accurate identification through instruments or rapid detection reagents.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent for fast identifying Wen county iron stick yam and identification method thereof
  • Reagent for fast identifying Wen county iron stick yam and identification method thereof
  • Reagent for fast identifying Wen county iron stick yam and identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The invention discloses a reagent for quickly identifying Wenxian Tiegon yam, the reagent is a glucose identification reagent, and the identification reagent and glucose undergo a color reaction.

[0035] The identification reagent is an alkaline buffer solution containing a water-soluble colorless tetrazolium salt.

[0036] The water-soluble colorless tetrazolium salt is 2,3,5-triphenyl tetrazolium chloride (red tetrazolium), 2,2'-diphenyl 5,5'-(3,4-dimethyl Oxyphenyl)-3,3'-dimethoxy-4,4'-bistetrazolium salt (tetrazolium blue), 2,5-diphenyl-3-(1-naphthyl)-2H -tetrazole chloride (tetrazole violet), 3,3'-(4,4'-biphenyl)bis(2,5-diphenyl-2H-tetrazole chloride) (neotetrazole chloride) One of them, or other structures. Different water-soluble colorless tetrazolium salts show different colors when identifying glucose.

[0037] Since glucose is a reducing sugar, the water-soluble colorless tetrazolium salt will be reduced to an insoluble colored formazan when it encounters ...

Embodiment 2

[0047] The invention discloses a reagent for quickly identifying Wenxian Tiegon yam, the reagent is a glucose identification reagent, and the identification reagent and glucose undergo a color reaction.

[0048] The identification reagent is an alkaline buffer solution containing a water-soluble colorless tetrazolium salt.

[0049] The water-soluble colorless tetrazolium salt is 2,3,5-triphenyl tetrazolium chloride (red tetrazolium).

[0050] The pH of the alkaline buffer solution is 10.

[0051] The pH of the alkaline buffer solution is adjusted by an aqueous sodium carbonate solution.

[0052]The preparation method of the alkaline buffer solution containing the water-soluble colorless tetrazolium salt is as follows: first prepare an aqueous solution of red tetrazolium with a mass concentration of 0.5%, add an alkaline buffer solution 3 times the volume of the aqueous solution, and then add the aqueous solution 10 times the volume of water, shake well. Wherein, the red tet...

Embodiment 3

[0056] The invention discloses a reagent for quickly identifying Wenxian Tiegon yam, the reagent is a glucose identification reagent, and the identification reagent and glucose undergo a color reaction.

[0057] The identification reagent is an alkaline buffer solution containing a water-soluble colorless tetrazolium salt.

[0058] The water-soluble colorless tetrazolium salt is 2,2'-diphenyl 5,5'-(3,4-dimethoxyphenyl)-3,3'-dimethoxy-4,4' - bistetrazolium salt (tetrazolium blue).

[0059] The pH of the alkaline buffer solution is 12.

[0060] The pH of the alkaline buffer solution is adjusted by an aqueous calcium hydroxide solution.

[0061] The preparation method of the alkaline buffer solution containing water-soluble colorless tetrazolium salt is as follows: first prepare an aqueous solution of tetrazolium blue with a mass concentration of 1%, add an alkaline buffer solution with 5 times the volume of this aqueous solution, and then add this aqueous solution for 5 Doubl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a reagent for fast identifying Wen county iron stick yam and an identification method thereof. The reagent is a glucose identification reagent; the identification reagent and glucose take chromogenic reaction. The identification reagent is alkaline buffer liquid containing water-soluble colorless tetrazolium salt. The water-soluble colorless tetrazolium salt is one of materials from red tetrazolium, blue tetrazolium, tetrazolium violet and neotetrazolium chloride. According to the characteristic that the content of glucose and fructose in the Wen county iron stick yam is small, the identification reagent capable of taking chromogenic reaction with glucose is used for identification; if the color development occurs, the result shows that the iron stick yam to be tested is not the Wen county iron stick yam; if the color development does not occur, the result shows that the iron stick yam to be tested is the Wen county iron stick yam. Compared with the prior art, the reagent and the identification method have the advantages that whether the yam is the Wen county iron stick yam or not can be fast identified; the operation is simple.

Description

technical field [0001] The invention relates to the technical field of crop detection, in particular to a reagent for rapidly identifying Wenxian Tiebang yam and an identification method thereof. Background technique [0002] Tiegu yam is a variety of yam, and Tiegu yam is the "extreme product" in Huai yam. According to the national standard "Geographical Indication Products Huai Yam (GB\T20351-2006)", it is a product of origin protected by the state. It is stipulated as The geographical range between 34.48 to 35.30 north latitude and 112.02 to 113.38 east longitude, that is, within the administrative jurisdiction of Jiaozuo, Henan Province, is recorded in "Wen County Chronicles" and "Agronomy Series" in the Qing Dynasty. It is valuable in food and medicine. . The iron stick yam has been applied by Jiaozuo as a national protected product of origin, and the place of origin is Wen County, Jiaozuo. [0003] The price of authentic Wenxian Tiegun yam is about 26 yuan per kilogr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 王强董春红李中贤郝佳李玉江王晓慧苏春燕刘陆平余学军李向力
Owner HIGH & NEW TECH RES CENT OF HENAN ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap